Figure 3.

Effect of UVA irradiation on p38MAP kinase and p53 signaling in SZ95 sebocytes

(A) Western blot analysis of phospho-p38 protein expression in SZ95 sebocytes after 1-2-4h post irradiation with UVA 2-5-8 J/cm² β-tubulin was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (control value taken as 1-fold in each case).

(B) Western blot analysis of p53 and p21 protein expression in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 2-5-8 J/cm². GAPDH was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (control value taken as 1-fold in each case).

(C) The mRNA expression levels of POMC, EDN1, SCF, and b-FGF in SZ95 sebocytes after 48h of treatment with PFTα 5 μM and one or 3-UVA 5 J/cm² irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed as the fold change respect to untreated control cells (∗p < 0.05, ∗∗p < 0.01 vs Ctr; ^$p < 0.05 vs 1-UVA irradiated cells; ^§p < 0.05 vs 3-UVA irradiated cells).

(D) Protein quantitation by ELISA of αMSH, EDN1, SCF, and b-FGF in SZ95 sebocytes after 48h of treatment with PFTα 5 μM and one or 3-UVA 5 J/cm² irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed in absolute quantities (∗∗p < 0.01 vs Ctr; ^$p < 0.05 vs 1-UVA irradiated cells; ^§p < 0.05 vs 3-UVA irradiated cells).