Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (AAV) | AAV2/1-EF1α-GCaMP6f-WPRE | FMI vector core | 6.0 × 1011–8.0 × 1012 GC/ml | |
| Strain, strain background (AAV) | AAV2/1-EF1α-Cre-t2a-mcherry-WPRE | FMI vector core | 3.2 × 1011–1.2 × 1013 GC/ml | |
| Strain, strain background (AAV) | AAV2/1-EF1α-Cre-WPRE | FMI vector core | 2.8 × 1010 GC/ml | |
| Strain, strain background (AAV) | AAV2/1-EF1α-NES-jRGECO1a-WPRE | FMI vector core | 4.8 × 1013 GC/ml | |
| Strain, strain background (AAV) | AAV2/1-CaMKIIα(1.3 kb)-mEGFP-P2A-paAIP2 | FMI vector core | 1.80 × 1013 GC/ml | |
| Strain, strain background (AAV) | AAV2/1-EF1α-DIO-mEGFP-P2A-paAIP2-WPRE | FMI vector core | 1.2 × 1013 GC/ml | |
| Strain, strain background (AAV) | AAV2/1-EF1α-mCherry- IRES-Flpo |
FMI vector core | 1.3 × 1013 GC/ml | |
| Strain, strain background (Mus musculus) | C57BL6/J | Charles River | ||
| Strain, strain background (M. musculus) | Grin1 tm2Stl/J | Jackson Laboratories | Cat# 005246 | |
| Strain, strain background (M. musculus) | Pvalb tm1(cre)Arbr | Jackson Laboratories | Cat# 008069 | |
| Strain, strain background (M. musculus) | Sst tm2.1(cre)Zjh | Jackson Laboratories | Cat# 018973 | |
| Chemical compound, drug | Fentanyl citrate | Actavis | CAS 990-73-8 | |
| Chemical compound, drug | Midazolam (Dormicum) | Roche | CAS 59467-96-8 | |
| Chemical compound, drug | Medetomidine (Domitor) | Orion Pharma | CAS 86347-14-0 | |
| Chemical compound, drug | Ropivacaine | Presenius Kabi | CAS 132112-35-7 | |
| Chemical compound, drug | Lidocaine | Bichsel | CAS 137-58-6 | |
| Chemical compound, drug | Buprenorphine | Reckitt Benckiser Healthcare | CAS 52485-79-7 | |
| Chemical compound, drug | Ophthalmic gel (Humigel) | Virbac | ||
| Chemical compound, drug | Flumazenil (Anexate) | Roche | CAS 78755-81-4 | |
| Chemical compound, drug | Atipamezole (Antisedan) | Orion Pharma | CAS 104054-27-5 | |
| Chemical compound, drug | N-Butyl-2-cyanoacrylate (Histoacryl) | Braun | CAS 6606-65-1 | |
| Chemical compound, drug | Dental cement (Paladur) | Heraeus Kulzer | CAS 9066-86-8 | |
| Chemical compound, drug | MK-801 | Sigma | CAS 77086-22-7 | |
| Software, algorithm | MATLAB (2020b) | MathWorks | RRID:SCR_001622 | |
| Software, algorithm | LabVIEW | National Instruments | RRID:SCR_014325 | |
| Software, algorithm | Two-photon acquisition software | Keller laboratory | https://sourceforge.net/p/iris-scanning/ | |
| Software, algorithm | Image data processing software | Keller laboratory | https://sourceforge.net/p/iris-scanning/calliope | |
| Software, algorithm | Python | https://python.org | RRID:SCR_008394 | |
| Software, algorithm | Panda3D | https://panda3d.org | ||
| Software, algorithm | R (v 4.0) | https://r-project.org | RRID:SCR_001905 | |
| Software, algorithm | LIGER (v 0.5) | (Welch et al., 2019) https://github.com/welch-lab/liger | RRID:SCR_018100 | |
| Software, algorithm | DropletUtils (v 1.8) | https://bioconductor.org | ||
| Software, algorithm | Seurat (v 3.1.5.9008) | https://satijalab.org | RRID:SCR_016341 | |
| Software, algorithm | Scater (v 1.16.0) | https://bioconductor.org | RRID:SCR_015954 | |
| Other | mRNA probe Mm-Grin1-O1 (probe region 2892–4127) | ACD bio | Cat# 473079 | |
| Other | Optogenetic-stimulation laser (OBIS 473 nm LX) | Coherent | Cat# 1187194 |