Protein	Imidazole pH 8.0 (mM)	Spin speeds (rpm)
α4 EC1–5	100	9000, 11,000, 13,000, 15,000
α7 EC1–5 L301R	100	9000, 11,000, 13,000, 15,000
α12 EC1–5 (poorly behaved)	200	11,000, 14,000, 17,000, 20,000
γB4 EC1–5	200	11,000, 14,000, 17,000, 20,000
γB5 EC1–4-AVI	200	11,000, 14,000, 17,000, 20,000
γC5 EC1–5 S116R	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4	100	9000, 11,000, 13,000, 15,000
β6 EC1–4-AVI tag	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 R41N	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 S117I	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 L125P	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 E369K	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 Y371F	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 R41N/S117I (precipitates)	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 R41N/E369K	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 S117I/L125P	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 R41N/S117I/L125P	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 R41N/S117I/E369K	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 R41N/E369K/Y371F	200	11,000, 14,000, 17,000, 20,000
β6 EC1–4 R41N/S117I/L125P/ E369K/Y371F	200	11,000, 14,000, 17,000, 20,000
β1 EC3–6	200	12,000, 16,000, 20,000, 24,000
β6 EC1–6	250	9000, 11,000, 13,000, 15,000
β9 EC3–6	200	11,000, 14,000, 17,000, 20,000
γA3 EC3–6	200	11,000, 14,000, 17,000, 20,000
γA9 EC3–6	200	11,000, 14,000, 17,000, 20,000
γB7 EC3–6 A570R	200	13,000, 17,000, 21,000, 25,000
αC2 EC3–6-AVI tag	200	11,000, 14,000, 17,000, 20,000
γC5 EC2–6	250	9000, 11,000, 13,000, 15,000
γC4 EC1–4	250	11,000, 14,000, 17,000, 20,000
γC4 EC1–4 D290A	250	11,000, 14,000, 17,000, 20,000
γC4 EC1–4 D290N	250	11,000, 14,000, 17,000, 20,000
γC4 EC1–4 E78A	250	11,000, 14,000, 17,000, 20,000
γC4 EC1–4 E78Q	250	11,000, 14,000, 17,000, 20,000
γC4 EC1–4 S344R	250	11,000, 14,000, 17,000, 20,000