| Mutant protein(γB7 numbering given in parentheses) | Cis interface side targeted | Protein expression in 25 ml test |
|---|---|---|
| γC3 EC3–6 Y540G (Y532G equivalent) | EC6-only | No |
| γC3 EC3–6 V560D (L555D equivalent) | EC6-only | No |
| γC3 EC3–6 V565R (V560R equivalent) | EC6-only | Yes |
| γC3 EC3–6 A575R (A570R equivalent) | EC5–6 | No |
| γC3 EC3–6 R563K (K558R equivalent) | Both | Yes |
| γA4 EC3–6 Y536G (Y532G equivalent) | EC6-only | No |
| γA4 EC3–6 L559D (L555D equivalent) | EC6-only | No |
| γA4 EC3–6 V564R (V560R equivalent) | EC6-only | Yes |
| γA4 EC3–6 A574R (A570R equivalent) | EC5–6 | No |
| γA4 EC3–6 K562R (K558R equivalent) | EC6-only | Yes |
| β1 EC3–6 V563R (V560R equivalent) | EC6-only | No |
| β1 EC3–6 S573R (A570R equivalent) | EC6-only | No |
| β1 EC3–6 K561R (K558R equivalent) | EC5–6 | No |
| β9 EC3–6 V563R (V560R equivalent) | EC6-only | No |
| β9 EC3–6 A573R (A570R equivalent) | EC6-only | No |
| β9 EC3–6 K561R (K558R equivalent) | EC5–6 | No |
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