FIG 6.

YBX1 is required for the assembly of intracellular infectious virions. Huh7 WT and YBX1 KO cells were infected with DENV at MOI 5. At 24 hpi, cell pellets were harvested and subjected to 5 cycles of freeze and thaw. Equal amounts of protein lysates were left untreated or treated with furin for 16 h at 30°C. (A) Representative Western blot analysis of prM and M expression upon furin treatment. (B) Titration of protein lysates was determined by plaque assay. Titers are expressed as PFU per milligrams of protein. Data are presented as mean ± SEM from three independent experiments.