Reference intervals for coagulation tests in adults with different ABO blood types

Zeliang Chen; Xiaoqing Dai; Jing Cao; Xuerui Tan; Shuying Chen; Min Yu

doi:10.1002/jcla.24269

. 2022 Feb 4;36(3):e24269. doi: 10.1002/jcla.24269

Reference intervals for coagulation tests in adults with different ABO blood types

Zeliang Chen ¹, Xiaoqing Dai ¹, Jing Cao ¹, Xuerui Tan ¹, Shuying Chen ^1,^✉, Min Yu ^1,^✉

PMCID: PMC8906010 PMID: 35119133

Abstract

Introduction

Coagulation tests are affected by many factors, such as age, race, and gestation. Although coagulation test results vary by ABO blood type, reference intervals of different ABO blood groups remain to be determined. This study aims to investigate the reference ranges of coagulation tests for different ABO blood groups in the Han population in South China.

Methods

A retrospective study was conducted in the First Affiliated Hospital of Shantou University Medical College. In all, 9600 individuals aged between 20 and 79 years were included. Coagulation tests, including prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), thrombin time, and fibrinogen, were performed.

Results

There was a significant difference in PT, INR, and aPTT among ABO blood groups. PT and INR varied slightly between ABO blood groups. There was a higher aPTT value in individuals in the O blood group than in those in non‐O blood groups, in both males and females across the included age range. No differences were found in thrombin time and fibrinogen between the ABO blood groups.

Conclusion

The study provides reference data on coagulation tests from ABO blood groups in South China. The established reference intervals specific to ABO blood type, sex, and age may improve clinical decisions based on coagulation tests.

Keywords: ABO blood group, activated partial thromboplastin time, coagulation tests, international normalized ratio, prothrombin time, reference intervals

The present study established age‐, sex‐, and ABO blood group‐specific reference intervals for coagulation tests. The individuals with blood type O had prolonged aPTT than those with type non‐O. APTT varied with age and sex. No differences were found between thrombin time and fibrinogen in the ABO blood groups.

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1. INTRODUCTION

Coagulation tests, mainly including prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), thrombin time (TT), and fibrinogen (FIB), are widely used in the clinic for anticoagulatant monitoring, evaluation of hemorrhage, and routine preoperative screening. ¹ , ² It is important to establish reference intervals to evaluate coagulation test results. Recently, associations between ABO blood type and coagulation test values have been shown. It was reported that there is a higher aPTT value in healthy volunteers with type O blood than in those with the non‐O blood types, ³ , ⁴ , ⁵ while PT does not differ among ABO blood groups. ⁵ Although coagulation tests are widely used and determination of whether the results are normal or abnormal has been interpreted using reference intervals, ⁶ , ⁷ reference intervals for coagulation tests did not consider the influence of ABO blood type on test results. Therefore, it is essential to establish ABO blood type‐specific reference intervals for coagulation tests.

Although there are 56 ethnic groups in China, the Han ethnic group accounted for 91.11% of the total population in 2020 during the seventh national census. ⁸ In Chaoshan District, 99% of the population was of the Han ethnic group. This study aimed to investigate the reference ranges of coagulation tests for ABO blood groups among the Han population in Chaoshan District of Guangdong Province in South China. Furthermore, the influences of age and sex on the results of coagulation tests were also investigated.

2. METHODS

A retrospective descriptive study was conducted in the First Affiliated Hospital of Shantou University Medical College. Between January 2015 and September 2019, coagulation testing was continuously reviewed, and results were collected from inpatients aged between 20 and 79 years who had not been diagnosed with diseases, including acute infections, severe hepatic or renal dysfunction, and coagulation abnormalities. The individuals were divided into groups according to their ABO blood type, age, and sex. A total of 9600 individuals were included. Coagulation tests included PT, INR, aPTT, TT, and FIB. The study was approved by the ethics committee of the First Affiliated Hospital of Shantou University Medical College (No. B‐2021‐203).

Data are expressed as the mean ± SD. Differences between group means were assessed by an unpaired Student's t test for single comparisons or by ANOVA for multiple comparisons using SPSS 25.0. A p value < 0.05 was considered to indicate statistical significance.

3. RESULTS

Among 9600 individuals with available ABO blood types, there were 2,400 people in each ABO blood group. As shown in Table 1, differences in age, TT, and FIB between ABO blood groups were not significant. Individuals with type O blood had prolonged PT and INR compared with individuals with non‐O blood types. There were no differences in PT and INR between the A, B, and AB blood groups. The differences in PT and INR between females and males were significant in the type A blood group but not in other blood groups. Although there were higher values of PT and INR in individuals with type O blood than in those with non‐O blood types, the differences were approximately 0.08 s and 0.008, respectively, which may have no clinical significance.

TABLE 1.

Coagulation assays of healthy adults with ABO blood types

ABO blood types	A	B	AB	O	p Value
Total number	2400	2400	2400	2400
Age (years)	49.438 ± 17.223	49.260 ± 17.133	49.327 ± 16.989	49.433 ± 17.034	0.980
PT (s)
Total	11.143 ± 0.607	11.160 ± 0.606	11.125 ± 0.599	11.208 ± 0.634	0.000
95% reference range	10.000~12.400	10.000~12.500	10.000~12.400	10.100~12.500
Male	11.195 ± 0.631	11.163 ± 0.607	11.134 ± 0.592	11.205 ± 0.631	0.020
Female	11.091 ± 0.578	11.157 ± 0.604	11.116 ± 0.606	11.211 ± 0.636	0.000
INR
Total	0.969 ± 0.053	0.971 ± 0.054	0.967 ± 0.053	0.975 ± 0.056	0.000
95% reference range	0.870~1.089	0.870~1.100	0.870~1.080	0.880~1.100
Male	0.974 ± 0.055	0.971 ± 0.054	0.968 ± 0.052	0.975 ± 0.055	0.011
Female	0.964 ± 0.051	0.970 ± 0.054	0.967 ± 0.054	0.975 ± 0.056	0.000
aPTT (s)
Total	28.289 ± 2.569	28.251 ± 2.529	28.116 ± 2.547	29.428 ± 2.553 ^a	0.000
95% reference range	24.400~33.700	24.400~33.500	24.300~33.700	25.300~34.300
Male	28.272 ± 2.558	28.211 ± 2.532	28.242 ± 2.601	29.468 ± 2.508 ^a	0.000
Female	28.306 ± 2.581	28.291 ± 2.528	27.991 ± 2.488 ^b	29.388 ± 2.598 ^a	0.000
TT (s)
Total	17.796 ± 1.437	17.718 ± 1.427	17.779 ± 1.512	17.777 ± 1.453	0.268
95% reference range	15.102~20.700	14.900~20.600	14.900~20.800	14.900~20.700
Male	17.775 ± 1.438	17.643 ± 1.431	17.739 ± 1.508	17.750 ± 1.482	0.132
Female	17.816 ± 1.435	17.792 ± 1.420	17.819 ± 1.515	17.805 ± 1.424	0.969
FIB (g/L)
Total	2.973 ± 0.547	2.973 ± 0.561	2.971 ± 0.551	2.980 ± 0.558	0.947
95% reference range	2.080~3.960	2.060~3.989	2.040~3.960	2.070~3.960
Male	3.003 ± 0.556	2.996 ± 0.560	3.003 ± 0.561	2.975 ± 0.564	0.590
Female	2.943 ± 0.536	2.951 ± 0.561	2.940 ± 0.539	2.985 ± 0.552	0.158

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Values are shown as the mean ± standard deviation.

Abbreviations: aPTT, activated partial thromboplastin time; FIB, fibrinogen; INR, international normalized ratio; PT, prothrombin time; TT, thrombin time.

^{^a}

O blood group vs. A, B, and AB blood groups, p < 0.05.

^{^b}

AB blood group vs. A and B blood groups, p < 0.05.

It has been demonstrated that individuals with type O blood have a higher aPTT than those with non‐O blood types. ³ , ⁴ , ⁵ Furthermore, aPTT varied with race. In the present study, individuals with type O blood also had significantly higher aPTT values than individuals with types A, B, and AB blood (29.428 ± 2.553 s vs. 28.289 ± 2.569 s, 28.251 ± 2.529 s, and 28.116 ± 2.547 s, respectively, p < 0.05). There was no difference in aPTT among the A, B, and AB blood groups. The 95% reference ranges of aPTT in the blood groups of A, B, AB, and O were 24.400~33.700 s, 24.400~33.500 s, 24.300~33.700 s, and 25.300~34.300 s, respectively. aPTT was affected by sex in blood group AB but not in the other ABO blood groups, with higher values in males than in females (as shown in Table 1).

The concentrations of most coagulation proteins vary significantly with age. ⁹ It was reported that aPTT was prolonged in infants ⁹ and children ¹⁰ compared with adults.

To investigate the effect of age on coagulation tests, coagulation assays were analyzed based on ages from 20 to 79 (as shown in Table 2). There were significant age‐related differences in aPTT in all ABO blood groups in females and in the A and O blood groups in males. Age‐, sex‐, and ABO‐specific reference intervals for aPTT, PT, INR, TT, and FIB in the A, B, AB, and O blood groups are shown in Figures 1, 2, 3, 4.

TABLE 2.

Coagulation assays of different ABO blood types

Gender	Male					Female
Blood types	A	B	AB	O	p Value	A	B	AB	O	p Value
Total number	1200	1200	1200	1200	p Value	1200	1200	1200	1200	p Value
PT (s)
20–29	11.229 ± 0.625	11.186 ± 0.588	11.142 ± 0.595	11.212 ± 0.628	0.509	11.141 ± 0.560	11.312 ± 0.584	11.153 ± 0.580	11.252 ± 0.645	0.010
30–39	11.155 ± 0.613	11.074 ± 0.589	11.116 ± 0.584	11.236 ± 0.616	0.049	11.086 ± 0.543	11.154 ± 0.589	11.083 ± 0.593	11.199 ± 0.590	0.132
40–49	11.181 ± 0.676	11.133 ± 0.576	11.189 ± 0.617	11.186 ± 0.655	0.795	11.118 ± 0.514	11.077 ± 0.536	11.113 ± 0.661	11.154 ± 0.587	0.614
50–59	11.068 ± 0.624	11.092 ± 0.627	11.139 ± 0.601	11.134 ± 0.593	0.599	10.988 ± 0.591	11.063 ± 0.587	11.113 ± 0.629	11.245 ± 0.704	0.001
60–69	11.221 ± 0.624	11.230 ± 0.621	11.137 ± 0.599	11.183 ± 0.631	0.422	11.025 ± 0.588	11.108 ± 0.646	11.156 ± 0.579	11.219 ± 0.607	0.012
70–79	11.315 ± 0.604	11.262 ± 0.624	11.082 ± 0.554	11.280 ± 0.661	0.001	11.187 ± 0.646	11.227 ± 0.647	11.082 ± 0.597	11.198 ± 0.676	0.120
INR
20–29	0.976 ± 0.054	0.972 ± 0.052	0.969 ± 0.052	0.975 ± 0.055	0.542	0.969 ± 0.049	0.984 ± 0.052	0.969 ± 0.051	0.979 ± 0.057	0.010
30–39	0.970 ± 0.054	0.963 ± 0.052	0.966 ± 0.051	0.978 ± 0.055	0.038	0.964 ± 0.049	0.970 ± 0.052	0.965 ± 0.052	0.974 ± 0.052	0.188
40–49	0.973 ± 0.060	0.968 ± 0.051	0.973 ± 0.054	0.973 ± 0.057	0.718	0.967 ± 0.044	0.963 ± 0.049	0.966 ± 0.059	0.969 ± 0.052	0.638
50–59	0.963 ± 0.055	0.964 ± 0.055	0.969 ± 0.052	0.967 ± 0.053	0.741	0.956 ± 0.049	0.962 ± 0.053	0.965 ± 0.056	0.979 ± 0.061	0.000
60–69	0.975 ± 0.055	0.978 ± 0.054	0.968 ± 0.053	0.973 ± 0.055	0.355	0.958 ± 0.053	0.967 ± 0.058	0.971 ± 0.052	0.976 ± 0.053	0.010
70–79	0.984 ± 0.053	0.979 ± 0.057	0.964 ± 0.049	0.982 ± 0.057	0.001	0.973 ± 0.056	0.977 ± 0.057	0.964 ± 0.053	0.973 ± 0.060	0.096
aPTT (s)
20–29	28.932 ± 2.620	28.507 ± 2.589	28.518 ± 2.664	30.110 ± 2.603 ^a	0.000	29.455 ± 2.572	29.484 ± 2.622	28.727 ± 2.657 ^b	30.150 ± 2.547 ^a	0.000
30–39	27.959 ± 2.602	28.089 ± 2.533	28.017 ± 2.581	29.357 ± 2.441 ^a	0.000	28.788 ± 2.586	28.743 ± 2.475	28.336 ± 2.479	30.079 ± 2.484 ^a	0.000
40–49	28.192 ± 2.445	27.881 ± 2.492	28.220 ± 2.762	29.368 ± 2.354 ^a	0.000	27.889 ± 2.155	28.230 ± 2.544	28.023 ± 2.501	29.358 ± 2.554 ^a	0.000
50–59	27.816 ± 2.347	28.021 ± 2.364	28.298 ± 2.641	29.126 ± 2.674 ^a	0.000	28.006 ± 2.640	27.897 ± 2.302	27.631 ± 2.390	28.906 ± 2.520 ^a	0.000
60–69	28.036 ± 2.584	28.291 ± 2.583	28.033 ± 2.433	29.189 ± 2.418 ^a	0.000	27.849 ± 2.585	27.601 ± 2.344	27.474 ± 2.304	28.779 ± 2.551 ^a	0.000
70–79	28.695 ± 2.574	28.476 ± 2.589	28.365 ± 2.507	29.662 ± 2.443 ^a	0.000	27.848 ± 2.507	27.790 ± 2.388	27.754 ± 2.386	29.058 ± 2.614 ^a	0.000
TT (s)
20–29	17.996 ± 1.547	17.798 ± 1.372	17.813 ± 1.510	17.897 ± 1.559	0.527	17.590 ± 1.361	17.581 ± 1.442	17.371 ± 1.632	17.720 ± 1.386	0.118
30–39	17.560 ± 1.361	15.584 ± 1.431	17.770 ± 1.546	17.704 ± 1.563	0.439	17.734 ± 1.465	17.698 ± 1.485	17.693 ± 1.461	17.450 ± 1.416	0.186
40–49	17.785 ± 1.492	17.555 ± 1.431	17.765 ± 1.455	17.847 ± 1.438	0.208	17.950 ± 1.379	17.868 ± 1.359	17.790 ± 1.396	17.888 ± 1.330	0.706
50–59	17.810 ± 1.359	17.759 ± 1.490	17.804 ± 1.483	17.770 ± 1.419	0.980	18.031 ± 1.419	17.912 ± 1.396	18.074 ± 1.431	18.009 ± 1.422	0.703
60–69	17.815 ± 1.383	17.637 ± 1.388	17.696 ± 1.481	17.745 ± 1.478	0.646	17.893 ± 1.414	17.896 ± 1.289	18.071 ± 1.517	17.969 ± 1.384	0.547
70–79	17.687 ± 1.459	17.528 ± 1.467	17.590 ± 1.578	17.540 ± 1.418	0.700	17.699 ± 1.533	17.801 ± 1.521	17.917 ± 1.541	17.794 ± 1.541	0.566
FIB (g/L)
20–29	2.882 ± 0.523	2.848 ± 0.555	2.854 ± 0.545	2.847 ± 0.545	0.908	2.824 ± 0.499	2.841 ± 0.529	2.851 ± 0.544	2.800 ± 0.535	0.784
30–39	2.966 ± 0.551	2.908 ± 0.573	2.929 ± 0.545	2.828 ± 0.564	0.087	2.780 ± 0.510	2.803 ± 0.541	2.794 ± 0.489	2.906 ± 0.534	0.063
40–49	2.934 ± 0.544	2.962 ± 0.524	2.936 ± 0.563	2.904 ± 0.559	0.776	2.834 ± 0.504	2.783 ± 0.500	2.846 ± 0.499	2.887 ± 0.520	0.231
50–59	3.023 ± 0.568	3.009 ± 0.552	3.110 ± 0.531	2.975 ± 0.560	0.093	2.998 ± 0.522	2.950 ± 0.540	2.937 ± 0.535	2.970 ± 0.534	0.681
60–69	3.051 ± 0.570	3.103 ± 0.547	3.047 ± 0.559	3.121 ± 0.539	0.434	3.018 ± 0.525	3.143 ± 0.553	3.044 ± 0.506	3.085 ± 0.525	0.096
70–79	3.160 ± 0.542	3.146 ± 0.559	3.141 ± 0.569	3.177 ± 0.527	0.912	3.201 ± 0.538	3.186 ± 0.564	3.165 ± 0.571	3.261 ± 0.543	0.350

Open in a new tab

Values are shown as the mean ± standard deviation.

Abbreviations: aPTT, activated partial thromboplastin time; FIB, fibrinogen; INR, international normalized ratio; PT, prothrombin time; TT, thrombin time.

^{^a}

O blood group vs. A, B and AB blood groups, p < 0.05.

^{^b}

AB blood group vs. A and B blood groups, p < 0.05.

Age‐ and sex‐specific 95% reference intervals for prothrombin time, international normalized ratio (INR), activated partial thromboplastin time (aPTT), thrombin time, and fibrinogen in the A blood group. 95% reference intervals for men (white boxes) and women (black boxes) for specific age groups

4. DISCUSSION

Coagulation tests are widely used in the clinic to detect hemostatic abnormalities and in preoperative evaluations. PT, aPTT, TT, INR, and FIB are commonly performed coagulation tests. Reference intervals are used to decide whether coagulation tests are normal or abnormal. Many factors, such as biological factors and methodological factors, affect reference intervals ¹¹ ; therefore, it is necessary to standardize these factors. However, it is impossible to eliminate the influence of sex, age, race, and similar factors on reference intervals, ¹¹ and dividing reference intervals into appropriate groups is important. Coagulation tests are also affected by sex, age, race, ³ , ⁴ , ⁵ and physiological differences such as gestation. ¹² , ¹³ Reference intervals of coagulation tests have been reported for pediatrics, adults, and gestation among different ethnic groups. ⁹ , ¹² , ¹³ , ¹⁴ , ¹⁵ , ¹⁶ , ¹⁷

Recently, associations between ABO blood groups and coagulation tests have been shown. It was reported that there were significantly prolonged values of aPTT in adults with type O blood than in those with non‐O blood types, ³ , ⁴ , ⁵ while the difference in PT among ABO blood groups was not significant. ⁵ However, the sample sizes in these studies were small, and reference intervals for different ABO blood groups were not known. Establishing reference intervals with large sample sizes is needed.

In the present study, we evaluated coagulation tests in 9600 Chinese individuals. We found that the individuals with blood type O had prolonged aPTT compared with those with non‐O blood types. aPTT in individuals aged between 20 and 79 with type O blood was higher than in those with non‐O blood types, in both males and females. aPTT was significantly higher in males than in females in the AB blood group. The study showed that ABO blood type‐specific reference values of aPTT are essential for the accurate interpretation of coagulation test results with individuals of different ABO blood groups.

There were higher PT and INR in the individuals with blood type O than in those with non‐O blood types in the present study, which is discordant with the previous study by Choi Q et al. in 2015, showing that PT was not affected by ABO blood group. ⁵ The mean PT and INR differed by approximately 0.08 s and 0.008, respectively, in those with type O blood versus those with type non‐O blood. It seems that significant differences in PT and INR among ABO blood groups were associated with the large sample size in the present study, which allows for the detection of minor variations. This difference may have no clinical significance.

The mechanisms of higher aPTT in individuals with blood type O are not well known. ABO blood type is associated with von Willebrand factor (vWF) and factor (F) VIII, with lower levels of vWF and FVIII in people with blood type O than in those with non‐O blood types. ¹⁸ , ¹⁹ It was reported that increased FVIII can shorten aPTT, ²⁰ which suggested that variations in aPTT can be partly explained by the ABO type. ⁵

There were some limitations in the present study. First, it was a retrospective study, and coagulation tests were collected from inpatients, which may differ from t a healthy population. We cannot exclude unknown factors affecting coagulation test results. Second, the individuals in the present study were aged from 20 to 79 years old. It was reported that the median time of aPTT and TT was significantly prolonged in all pediatric age groups compared with the adult group in a previous study ¹⁰ ; therefore, reference values of the individuals aged below 20 years remained to be determined. Finally, due to race‐based differences in aPTT, ²¹ the reference range of coagulation assays in a healthy population in the Chaoshan region may be different from that in other populations in China.

5. CONCLUSION

In summary, the present study established ABO blood type‐specific reference intervals for coagulation tests and provided age‐ and sex‐related reference intervals. The reference intervals in the present study showed significant variation in aPTT with ABO blood groups, suggesting that ABO blood groups should be evaluated using reference ranges of coagulation tests.

CONFLICT OF INTEREST

There are no conflicts of interest.

AUTHORS CONTRIBUTIONS

ZLC drafted the manuscript. XQD and JC performed the experiments. XRT analyzed the data. SYC and MY conceived the study and edited the manuscript. All authors read and approved the final manuscript.

ACKNOWLEDGEMENTS

This work was supported by the Medical and Health Science and Technology Planning Project of Shantou (Fourth Project in 2021) and the Medical Scientific Research Foundation of Guangdong Province (No A2018125).

Chen Z, Dai X, Cao J, Tan X, Chen S, Yu M. Reference intervals for coagulation tests in adults with different ABO blood types. J Clin Lab Anal. 2022;36:e24269. doi: 10.1002/jcla.24269

Contributor Information

Shuying Chen, Email: csy677553@163.com.

Min Yu, Email: 717146@sina.com.

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available on request from the corresponding author.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The data that support the findings of this study are available on request from the corresponding author.

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PERMALINK

Reference intervals for coagulation tests in adults with different ABO blood types

Zeliang Chen

Xiaoqing Dai

Jing Cao

Xuerui Tan

Shuying Chen

Min Yu