Table 2.
Characteristics of the PRP injection used in each trial
| Study | Kit | PRP preparation | Anticoagulant used | Centrifugation technique | Leucocytes concentration (LR- or LP-PRP) | Platelets concentration ×103 | Platelet concentration (compared to baseline) | Activating agent |
|---|---|---|---|---|---|---|---|---|
| Barreto et al. (2019) [14] | Coleman 80-2C Macro Centrifuge | 15 mL venous blood resulted in 3 mL PRP | NR | Double centrifugation; 1,500 RPM for 6 minutes and then 3,500 RPM for 15 minutes | LR-PRP | NR | <5 (3 times) | NR |
| Cai et al. (2019) [15] | NR | 20 mL venous blood resulted in 5–6 mL PRP | Sodium citrate | Double centrifugation; | LP-PRP | 1×1012 L–1 | NR | NR |
| 1,500 RPM for 10 minutes (4℃) followed by 2,500 RPM for 10 minutes (4℃) | ||||||||
| Ilhanli et al. (2015) [16] | NR | 15 mL venous blood resulted in 6 mL PRP | Citrate dextrose | Single centrifugation; 3,500 RPM for 9 minutes | NR | NR | <5 (2.1–2.5 times) | Calcium chloride |
| Kesikburun et al. (2013) [17] | GPS III system (Biomet Biologics) | 54 mL venous blood resulted in 6 mL PRP | Citrate | Single centrifugation; 3,200 RPM for 15 minutes | LR-PRP | (1,014.9± 340.2)×103 µL | <5 (4 times) | Nil |
| Kothari et al. (2017) [18] | Research Centrifuge REMI | 20 mL venous blood resulted in 2 mL PRP | CDPA | Single centrifugation; 200 RPM for 10 minutes | NR | NR | ≥5 (6 times) | Nil |
| Nejati et al. (2017) [19] | Tubex Autotube system (Moohan Enterprise) | 25 venous blood resulted in 5 mL PRP | Citrate dextrose | Double centrifugations; 1,300 RPM for 10 minutes followed by 2,770 RPM for 8 minutes | LR-PRP | 900,000±15,000/mm3 | <5 (3 times) | NR |
| Pasin et al. (2019) [20] | Neotec Biotechnology PRP kit (Elektro-Mag) | 8.5 mL venous blood resulted in 4 mL PRP | Citrate | Double centrifugation; 1,200 g for 5 minutes followed by 1,200 g for 10 minutes | NR | NR | NR | Nil |
| Rha et al. (2013) [21] | Prosys PRP system (Tozaiholdings) | 25 mL venous blood resulted in 3 mL PRP | Citrate dextrose | Double centrifugation; 1,600 G then 2,000g | NR | NR | NR | NR |
| Sari and Eroglu (2020) [22] | NR | 100 mL venous blood resulted in 10 mL PRP | Sodium citrate | Double centrifugation; 1,500 RPM for 6 min followed by 3,500 RPM for 12 minutes | NR | NR | ≥5 (5 times) | calcium chloride |
| Schwitzguebel et al. (2019) [23] | RegenKit BCT (Regen Lab) | 8 mL venous blood resulted in 2 mL PRP | NR | Single centrifugation; 1,500 RPM for 5 minutes | LP-PRP | (343.3±89.4) ×106/mL | <5 (1.6 times) | Nil |
| Shams et al. (2016) [24] | MyCells (ProTech, Kaylight) | 10 mL venous blood resulted in 2–2.5 mL PRP | Citrate dextrose | Single centrifugation; 3,500 RPM for 10 minutes | LR-PRP | NR | NR | NR |
| Šmíd et al. (2018) [27] | Centurion Scientific C2 | 30 mL venous blood resulted in 6 mL PRP | Citrate dextrose | Single centrifugation; | LP-PRP | 459,000/mL | <5 (2–2.5 times) | NR |
| centrifugation force of 150 g for 10 minutes at 20℃ | ||||||||
| Upadhyay et al. (2020) [28] | REMI Centrifuge C-854/6 System | 15 mL venous blood resulted in 2 mL PRP | Ethylenediaminetetraacetic acid | Single centrifugation; | NR | NR | NR | Calcium chloride |
| 1,500 RPM for 15 minutes | ||||||||
| Wesner et al. (2016) [11] | Harvest System | NR | NR | Single centrifugation; | NR | NR | NR | NR |
| centrifugation rate: NR | ||||||||
| Jeyaraman et al. (2018) [29] | Remi 8 centrifuge | NR | NR | Double centrifugation; | NR | NR | ≥5 (5–6 times) | Calcium chloride |
| 3,000 RPM for 10 minutes and 5,000 RPM for 10 minutes | ||||||||
| Ibrahim et al. (2019) [26] | NR | 20 mL venous blood resulted in 2 mL PRP | Sodium citrate | Double centrifugation; | NR | NR | NR | Thrombin |
| 700–1,500 RPM for 15–20 minutes and 2,500-3,500 RPM for 10 minutes | ||||||||
| Wongworawat (2013) [25] | Magellan Autologous Platelet Separator System | 45 mL venous blood resulted in 5 mL PRP | NR | NR | NR | NR | NR | NR |
PRP: platelet-rich plasma, LR: leucocyte rich, LP: leucocytes poor, NR: not reported, RPM: revolutions per minute, CDPA: Citrate Phosphate Dextrose Adenine Solution.