Table 1.
Taxonomic affiliation of the six microalgal strains newly isolated from regional freshwater environments.
| Strain | Isolation Source | Sequenced Gene | Accession Number | Length (bp) | Best Blast Hit (Identity %) |
Taxonomic Affiliation |
|---|---|---|---|---|---|---|
| F1 | “Le Morette”, Fucecchio Marshland | 18S rDNA | OM311002 | 2919 | Desmodesmus brasiliensis AB917106 (94.53) | Scenedesmaceae |
| Final portion of 18S, complete ITS1-5.8S-ITS2, initial portion of 28S rDNA | OM310999 | 1153 | Desmodesmus subspicatus MK975481 (96.63) | |||
| F2 | “Le Morette”, Fucecchio Marshland | 18S rDNA | OM311003 | 1703 | Chlamydomonas debaryana MF678003 (99.82) | Chlamydomonas debaryana |
| F3 | “Le Morette”, Fucecchio Marshland | 18S rDNA | OM311004 | 1716 | Chlamydomonas debaryana MF678003 (99.94) | Chlamydomonas debaryana |
| F4 | “Le Morette”, Fucecchio Marshland | 18S rDNA | OM311005 | 1758 | Chlorella sorokiniana MF101221 (100) | Chlorella sorokiniana |
| Final portion of 18S, complete ITS1-5.8S-ITS2, initial portion of 28S rDNA | OM311000 | 738 | Chlorella sorokiniana KM514859 (99.59) | |||
| R1 | Private terrace in Pisa, water sample | 18S rDNA | OM311006 | 1758 | Chlorella sorokiniana MF101221 (99.89) | Chlorella sorokiniana |
| C2 | Heating system of a private house | 18S rDNA | OM311001 | 1704 | Chlorella sp. KP262476 (99.30) | Chlorella-like microalgal strain |
| Final portion of 18S, complete ITS1-5.8S-ITS2, initial portion of 28S rDNA | OM310998 | 1279 | Chlorella vulgaris KT778121 (99.70) |