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. 2022 Jan 29;41(11):1622–1633. doi: 10.1038/s41388-022-02185-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Analysis of H3K4me3 modification in the METTL3 locus in HuCC-T1 cell (datasets from the Cistrome Data Browser). B SETD1B expression in ICC tumors (n = 36) and normal tissues (n = 9) in the GEPIA2 database. C Correlation analysis of METTL3 expression with SETD1B expression in our ICC cohort. D The mRNA levels of SETD1B in SETD1B-KD and control HCCC-9810 cells were confirmed by RT-qPCR. E The mRNA levels of METTL3 in SETD1B-KD and control HCCC-9810 cells were confirmed by RT-qPCR. F The protein levels of METTL3 and H3K4me3 modification after SETD1B silencing in HCCC-9810 cells were confirmed by western blotting. G The mRNA level of METTL3 after CPI-455 treatment for 72 h in HuCC-T1 and HCCC-9810 cells was confirmed by RT-qPCR. H Chromatin immunoprecipitation-qPCR analysis of H3K4me3 enrichment in the METTL3 locus or a flank region with no signal in SETD1B-KD or control HCCC-9810 cells. NT = A flank region with no signal was used as a negative control. The results are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, according to Student’s t test.