Fig. 1.

Cell death analysis and colony forming of ALK/ROS1-wt BEAS-2B and A549 lung cells. Cells were treated with (a) alectinib or (b) crizotinib, 2 Gy irradiation or a combination of KI + IR. Cells died dose-dependent and showed higher cell death rates after crizotinib, unaffected by irradiation (p < 0.050). Additionally, relative number of colonies was measured after (c) alectinib and (d) crizotinib (non-linear curve fit). Cells died irradiation- and kinase inhibitor-dependent (p < 0.050; one-tailed Mann-Whitney-U). (e) Normalization of colony forming data showed additive effects of the combinatory treatment. (f) Homologous recombination status: staining of γH2AX and RAD51 foci of untreated controls and CC-115 treated (DNA-PK inhibitor) cells. Each value represents mean ± SD (n = 4). Significance was determined by two-tailed Mann Whitney U test * p ≤ 0.050.