| Transfected construct (Gallus gallus) |
shRNA: Npnt |
This paper |
|
See Materials and methods, section ‘Production of RCAS virus’ |
| Transfected construct (G. gallus) |
shRNA: Itga8 |
This paper |
|
See Materials and methods, ‘Production of RCAS virus’ |
| Biological sample (G. gallus) |
Primary periocular neural crest |
This paper |
|
See Materials and methods, section ‘In vitro explant culture’ |
| Biological sample (G. gallus) |
DF-1 cells |
ATCC |
Cat# CRL12203
|
Lot# 58217603, no mycoplasma contamination detected |
| Antibody |
Anti-Npnt (rabbit polyclonal) |
Biorbyt |
Cat# orb221700; RRID:AB_2905548
|
IF (1:100) |
| Antibody |
Anti-GFP (mouse monoclonal) |
Invitrogen |
Cat# A-6455; RRID:AB_221570
|
IF (1:500) |
| Antibody |
Anti-fibronectin (mouse monoclonal) |
DHSB |
Cat# B3/D6; RRID:AB_2105970
|
IF (1:30) |
| Antibody |
Anti-phosphorylated paxillin (rabbit polyclonal) |
Invitrogen |
Cat# 44-722G; RRID:AB_2533733
|
IF (1:200) |
| Antibody |
Anti-BrdU (mouse monoclonal) |
DHSB |
Cat# G3G4; AB_2618097 |
IF (1:30) |
| Chemical compound, drug |
Bromodeoxyuridine |
Sigma |
Cat# B5002 |
|
| Chemical compound, drug |
Poly-d-lysine |
Sigma |
Cat# P6407 |
|
| Recombinant DNA reagent |
pSLAX13 |
Addgene |
Cat# CT#232 |
|
| Recombinant DNA reagent |
RCAS plasmid |
Hughes et al., 1987;doi.org/10.1128/jvi.61.10.3004–3012.1987
|
|
|
| Recombinant RNA reagent |
Integrin α8 RNA probe |
This paper |
PCR primers |
Supplementary file 1
|
| Recombinant RNA reagent |
Nephronectin RNA probe |
This paper |
PCR primers |
Supplementary file 1
|
| Peptide, recombinant protein |
α8β1 inhibitor |
Sato et al., 2009;doi.org/10.1074/jbc.M900200200
|
GenScript |
|
| Peptide, recombinant protein |
Nephronectin (human) |
R&D Systems |
9560NP-050 |
1.5 µg/cm2
|
| Peptide, Recombinant protein |
Nephronectin (mouse) |
R&D Systems |
AF4298-NP-50 |
1.5 µg/cm2
|
| Commercial assay or kit |
CloneEZ PCR cloning kit |
GenScript |
Cat# L00339
|
|
| Commercial assay or kit |
DIG RNA Labelling Kit (SP6/T7) |
Roche |
Cat# 11175025910 |
|
| Chemical compound, drug |
PF-573228 |
Sigma-Aldrich |
Cat# PZ0117-5MG |
10 µM |
| Chemical compound, drug |
Y27632 |
Sigma-Aldrich |
Cat# Y0503 |
10 µM |
| Other |
DAPI stain |
Roche |
D8417 |
(1 µg/mL) |
| Other |
Phalloidin |
Invitrogen |
A-12380 |
(1:200) |
