Table 2.

The information of primers in the CNV and mRNA expression test

Primersa	Sequence	Locationb	Amplified fragment (bp)
DMBT1-CNV	F: 5′- TGAGCCATAACACAGGCATCTGA-3′ R: 5′- TGGTAATATAAACACCTGGACTCGG-3′	Chr26: 42789218–42789415	198
BTF3	F: 5′- AACCAGGAGAAACTCGCCAA -3′ R: 5′- TTCGGTGAAATGCCCTCTCG -3	Chr20: 8044271–8044436	166
DMBT1-qPCR	F: 5′-ACGAGCTACCTCCAATCCCT -3′ R: 5′- TGGTGTCGTTATCCACCTGC -3	XM_024985978.1	160
MYOG-qPCR	F: 5′- CGAGTGCCCCTTGAAGACAA -3′ R: 5′- TACACACCTTACACGCCCAC -3	NM_001111325.1	82
β-actin-qPCR	F: 5′- GTCATCACCATCGGCAATGAG -3′ R: 5′- AATGCCGCAGGATTCCATG -3	NM_173979.3	84

^aPrimers of DMBT1-CNV and BTF3 were used to test the CNV of DMBT1 gene; primers of DMBT1-qPCR, MYOG-qPCR, and β-actin-qPCR were used to test the mRNA expression level

^bThe locations were according to the reference genome and mRNA sequence from NCBI database (Assembly Bos_taurus_UMD_3.1.1.)