| Gene (Homo sapiens) |
FBXW7
|
NCBI |
Gene ID: 55294 |
Also known as Fbw7 and FBXW7 |
| Gene (H. sapiens) |
JUN
|
NCBI |
Gene ID: 3725 |
Also known as AP1, AP-1, cJUN, and c-Jun |
| Gene (H. sapiens) |
MYC
|
NCBI |
Gene ID: 4609 |
Also known as MYC, c-Myc, cMyc |
| Cell line (H. sapiens) |
Hct116 |
From Dr. Julian Simon at Fred Hutch |
HCT116 CCL-247 |
Adult male colon cancer |
| Cell line (H. sapiens) |
U5 human neural stem cells (NSCs) |
Jackson Laboratory |
B6.129P2Gpr37tm1Dgen/J |
Primary cells |
| Antibody |
Anti-H3K27ac Rabbit Monoclonal |
Abcam |
Cat#: ab45173 |
C&R (1:100) |
| Antibody |
Anti-H3K27me3 Rabbit Monoclonal(C36B11) |
Cell Signaling Technology |
Cat#: 9733A |
C&R (1:100) |
| Antibody |
Anti-cJun Rabbit Polyclonal (H-79) |
Santa Cruz Biotechnology |
Cat#: sc-1694 |
C&R (1:25) |
| Antibody |
Anti-Myc Rabbit Monoclonal(D3N8F) |
Cell Signaling Technology |
Cat#: 13987S |
C&R (1:25) |
| Antibody |
Anti-H3K4me1Rabbit Polyclonal |
Abcam |
Cat#: ab8895 |
C&R (1:100) |
| Antibody |
Anti-H3K4me2 Rabbit Monoclonal(C64G9) |
Cell Signaling Technology |
Cat#: 9725 |
C&R (1:100) |
| Antibody |
Normal IgG (Rabbit) |
Santa Cruz Biotechnology |
sc-2027 |
C&R (1:50) |
| Antibody |
Anti-Fbw7 Rabbit Polyclonal |
Bethyl |
A301-720A |
WB (1:1000) |
| Antibody |
Anti-HLA DR Mouse MonoclonalTAL 1B5 |
Abcam |
ab20181 |
WB (1:500) |
| Antibody |
Anti-HLA DR+ DP + DQ Mouse Monoclonal CR3/43 |
Abcam |
ab7856 |
WB (1:500) |
| Antibody |
Anti-Mouse IgG peroxidase-linked secondary |
Cytiva NA9311ML |
Cat#: 45-000-679 |
WB (1:10,000) |
| Antibody |
Anti-Rabbit IgG peroxidase-linked secondary |
Cytiva NA9341ML |
Cat#: 45-000-682 |
WB (1:10,000) |
| Antibody |
Anti-γ Tubulin Mouse Monoclonal |
Santa Cruz Biotechnology |
sc-17787 |
WB (1:1000) |
| Antibody |
Anti-H3 Mouse Monoclonal (96C10) |
Cell Signaling Technology |
Cat#: 3638S |
WB (1:1000) |
| Antibody |
PE Anti-HLA DR+ DP + DQ Mouse Monoclonal WR18 |
Abcam |
ab23901 |
FC (1:50) |
| Antibody |
PE Anti-HLA DR Mouse Monoclonal |
BioLegend |
Cat#: 307605 |
FC (1:50) |
| Commercial assay or kit |
QIAGEN RNeasy Mini Kit |
QIAGEN |
Cat#: 74104 |
|
| Commercial assay or kit |
iScript Reverse Transcription Supermix |
Bio-Rad |
Cat#: 1708841 |
|
| Commercial assay or kit |
Platinum SYBR Green qPCR SuperMix-UDG with Rox |
Invitrogen |
Cat#: 11744100 |
|
| Software, algorithm |
Beckman Biomek FX liquid handling robot |
Genomics and Bioinformatics Center at Fred Hutch |
|
Automated CUT&RUN |
| Software, algorithm |
ChemiDoc Touch Imaging System OS 2.3.0.07 |
Bio-Rad |
|
|
