Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 14;13(1):530–541. doi: 10.1080/21505594.2022.2040190

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Construction of recombinant virus. (a) the multi-basic amino acid motif located at the HA cleavage site were removed and the HA2 specific peptide (⁴⁶³ADSEMDKLYERVKRQLRENA⁴⁸²) were replaced by H3 subtype 12 peptide (⁴⁶³ADSEMNKLFEKTKKQLRENA⁴⁸²). (b) the recombinant HA_Lo-mH3 and the NA plasmid of GD4, combined with high-yield viral backbone from H9N2 subtype TX strain (containing PB2, PB1, PA, NP, M, and NS plasmids), was used to construct a recombinant virus based on our established reverse genetic manipulation.