Key Resources Table

Reagent or Resource	Source	Identifier
RNAscope in situ Hybridization Probes
Grm5 (target region 2409-3336)	ACDBio	NM_001081414.2
Sst (target region 18-407)	ACDBio	NM_009215.1
Pvalb (target region 2-885)	ACDBio	NM_013645.3
Viruses
AAV5-hSyn-hChR2(H134R)-EYFP (7e12 vg/mL)	unpublished	RRID:Addgene_26973
AAV5-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (1e13 vg/mL)	unpublished	RRID:Addgene_20298
AAV5-Syn-ChrimsonR-tdT (7e12 vg/mL)	(Klapoetke et al., 2014)	RRID:Addgene_59171
AAV9-syn-FLEX-jGCaMP7f-WPRE (1e13 vg/mL)	(Dana et al., 2019)	RRID:Addgene_104492
AAV10-CAG-FLEX-HaloTagDART2.0-2A-dTomato-WPRE (2e12 vg/mL)	MR Tadross	N/A
AAV10-CAG-FLEX-ddHaloTagDART2.0-2A-dTomato-WPRE (2e12 vg/mL)	MR Tadross	N/A
Chemicals
(S)-3,5-DHPG (100 μM)	HelloBio	Cat # HB0045
D-AP5 (50 μM)	Tocris	Cat # 0106
CNQX (20 μM)	Tocris	Cat # 1045
Picrotoxin (1 μM in pipette)	Tocris	Cat # 1128
Muscimol (10 μM)	HelloBio	Cat # HB0887
MTEP (1 μM)	Tocris	Cat # 2921
VU0469650 (10 μM)	Tocris	Cat # 537950
BAPTA (20 mM in pipette)	Sigma	Cat # 14510
NASPM (200 μM)	HelloBio	Cat # HB0441
YM90K-DART_2.0 (0.2 - 1 μM)	MR Tadross	N/A
Alx647-DART_2.0 (0.02 – 0.1 μM)	MR Tadross	N/A
Experimental Models
Mouse: C57BL/6J	The Jackson Laboratory	000664
Mouse: mGluR5-loxP; B6.129-Grm5tm1.1Jixu/J	The Jackson Laboratory	028626
Mouse: SST Cre; Ssttm2.1(cre)Zjh/J	The Jackson Laboratory	013044
Mouse: PV Cre; B6;129P2-Pvalbtm1(cre)Arbr/J	The Jackson Laboratory	017320
Mouse: Ai9; B6.Cg-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J	The Jackson Laboratory	007909
Software and Algorithms
pClamp	Molecular Devices	RRID:SCR_011323
Prism 8.0	GraphPad Software	RRID:SCR_002798
DeepLabCut 2.1.5	DeepLabCut	https://github.com/AlexEMG/DeepLabCut
MATLAB 2019a	Mathworks	RRID:SCR_001622
Fiber photometry analysis code	custom code	10.5281/zenodo.5777173
Synapse Suite	Tucker-Davis Technologies	https://www.tdt.com/component/synapse-software/
RStudio	RStudio, Inc.	RRID:SCR_000432
R	R Foundation	RRID:SCR_001905
Fiji	NIH	RRID:SCR_002285