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. 2022 Feb 9;41(12):1821–1834. doi: 10.1038/s41388-022-02212-1

Fig. 7. PSD4 plays a prometastatic role in HCC progress in vitro and in vivo.

Fig. 7

A Immunohistochemical staining (IHC) and expression scoring of PSD4 was performed in 169 HCC tissues. Representative pictures were shown (scale bar: 200 μm). B Spearman correlation analysis between PSD4 and DDR1 expression. N = 169 (right panel). C, D Kaplan–Meier analysis was used to illustrate the correlation between PSD4 expression and overall survival or disease-free survival of HCC patients. The cutoff for determining low or high PSD4 expression was the median value. E Analysis of the activation level of ARF6 in indicated SK-Hep1 and HLF cells. F, H Lung metastasis from nude mice injected with SK-Hep1 and HLF cells by tail veins from both groups killed at 8 weeks, was measured by bioluminescent imaging (BLI), representative images of lung tissue sections, and number of lung metastatic foci in both groups. G, I Representative hematoxylin and eosin staining of lung tissue sections, and incidence of lung metastasis in both groups of BALB/c (nu/nu) mice. J Analysis of the activation level of ARF6 in indicated Hep3B cells with collagen I. K Proposed oncogenic DDR1/PSD4/ARF6 signaling pathway in HCC metastasis. *P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001: the scramble group compared with the shPSD4#3 group.