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. 2022 Feb 10;11:e71928. doi: 10.7554/eLife.71928

Figure 5. mib1 interacts with wnt5b to control Convergence Extension movements.

(A) Analysis of the axis extension angle α in bud stage embryos reveals that a subliminal dose of wnt5b morpholino (MO wnt5b) that has no effect on Convergent Extension (CE) in WT significantly enhances the defects observed in animals injected with mib1 morpholino (MO mib1). (B,C) wnt5b morpholino injection impairs CE in mib1tfi91 WT siblings (B) and enhances CE defects in mib1tfi91 homozygous mutants (C). (A–C) Lateral views of bud stage embryos, anterior up, dorsal to the right. Scalebars 200 µm. Boxes represent mean values ± SD. See Figure 5—source data 1 for complete statistical information.

Figure 5—source data 1. Complete statistical information for the experiments reported in Figure 5.

Figure 5.

Figure 5—figure supplement 1. Mib1 is dispensable for Wnt5b-induced Ryk degradation.

Figure 5—figure supplement 1.

(A,B) 17 out of 18 Wnt5b-injected embryos present severely reduced Ryk-GFP signals compared to WT controls (n = 12). (C,D) A similar Wnt5b-dependent decrease of Ryk-GFP levels is observed in mib1tfi91 homozygous mutants that were additionally injected with dominant-negative Mib1ΔRF123 (n = 14 mutant and n = 16 WT control embryos). All pictures depict dorsal views of 90% epiboly stage embryos, anterior up. The Histone2B-mRFP signal (A’-D’) was used to ascertain that control and Wnt5b-expressing embryos had received comparable amounts of injected material. Scalebars: 10 µm.