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. Author manuscript; available in PMC: 2022 Nov 19.
Published in final edited form as: Science. 2021 Nov 18;374(6570):1005–1009. doi: 10.1126/science.abj6749

Fig. 2. FlcB catalyzes S-succinylation of cysteine, and FlcE performs oxidative decarboxylation.

Fig. 2.

(A) Feeding studies in P. aeruginosa PAO1 using [15N,13C5] l-methionine or [15N,13C3] l-cysteine. Mass spectra of fluopsin C with a single ligand are shown. Positions of isotopic labeling are inferred. (B) EICs of 2 (m/z 238.0380 [M+H]+) from the FlcB reaction and controls. A small amount of racemic 2 forms nonenzymatically. (C) FlcE converts 2 to 3 in an Fe(II)-dependent manner. EICs of 3 (m/z 230.0094 [M+Na]+) are shown. (D) NMR spectrum of 3 in deuterated dimethylsulfoxide supports an oxime structure that exists in two diastereomers. HMBC, heteronuclear multiple bond correlation; ppm, parts per million.