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. 2022 Feb 26;25(4):103993. doi: 10.1016/j.isci.2022.103993

Figure 2.

Figure 2

AST1 alters the timing of appearance of proliferating cells in the niche

(A) Comparison of the % chance of finding BrdU-labeled cells in the niche after a single BrdU injection on day 0 (purple, peaks A and B; graph adapted from Benton et al., 2014), and after simultaneous injection of BrdU plus r-AST1 on day 0 (red dashed graph, peaks C–F). For both experiments, animals were euthanized and brains dissected on days 1–8, 10, 14, and 21. Peaks in labeling are denoted by letters and are found on days 2 and 10 for the BrdU alone injection (Peaks A and B), and on days 2, 4, 7, and 10 for the BrdU plus r-AST1 injection (peaks C–F, indicated above each peak).

(B) The average number of BrdU-labeled cells in the niche (right Y axis) was calculated for each time point (bar graphs; mean ± SD) for the BrdU plus r-AST1 injection, and plotted with the % chance of finding a BrdU-labeled cell in the niche (left Y axis).