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Ultraviolet–visible spectra and a 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) gel of purified ScTYW1. The black trace is purified and reconstituted ScTYW1; the blue dashed trace is after the addition of dithionite, and the purple dotted trace is for the supernatant following TCA treatment. Lanes 1 and 2 of the SDS–PAGE gel contain 5 and 10 μg of protein, respectively.
