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. Author manuscript; available in PMC: 2022 Mar 24.
Published in final edited form as: Bone. 2021 Jun 15;151:116058. doi: 10.1016/j.bone.2021.116058

Fig. 3.

Fig. 3.

Functional integrity assessment of purified mitochondria harvested from MSCs. Three independent cultures of MSCs were utilized and mitochondria were purified from cells at day 0 or at day 7 and 14 following osteogenic induction. Mitochondria from each time point were pooled and stained (or not) with TMRE. Histograms from FACS analysis revealed the distribution of unstained (red) and TMRE-stained (blue) mitochondria. Utilizing corresponding FACS analysis software, we calculated that approximately 92%, 90% and 86% of mitochondria stained positive for TMRE in day 0, day 7 or day 14 samples, respectively.