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Analysis of four differentially-expressed miRNAs in whole cell extracts by RT-qPCR. RNA from the same three whole cell extracts analyzed via microarray were also analyzed by RT-qPCR. Expression levels of miR-4485 (A), miR-181a-5p (B), miR-483-5p (C), miR-138-5p (D) and miR-20a-5p (E) were normalized to RNU44 and plotted as fold change expression compared to day 0. Data are expressed ± SD; n = 3. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
