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. 2022 Mar 2;11(1):18. doi: 10.3390/antib11010018

Ramifications of the HLA-I Allelic Reactivity of Anti-HLA-E*01:01 and Anti-HLA-E*01:03 Heavy Chain Monoclonal Antibodies in Comparison with Anti-HLA-I IgG Reactivity in Non-Alloimmunized Males, Melanoma-Vaccine Recipients, and End-Stage Renal Disease Patients

Mepur H Ravindranath 1,2,*, Narendranath M Ravindranath 3, Fatiha El Hilali 4, Senthamil R Selvan 5, Edward J Filippone 6
Editor: Itai Benhar
PMCID: PMC8944535  PMID: 35323192

Abstract

Serum anti-HLA-I IgG are present in non-alloimmunized males, cancer patients, and transplant recipients. Anti-HLA-I antibodies are also present in intravenous immunoglobulin (IVIg), prepared from the plasma of thousands of healthy donors. However, the HLA-Ia reactivity of IVIg diminishes markedly after passing through HLA-E HC-affinity columns, suggesting that the HLA-I reactivity is due to antibodies formed against HLA-E. Hence, we examined whether anti-HLA-E antibodies can react to HLA-I alleles. Monoclonal IgG antibodies (mAbs) against HCs of two HLA-E alleles were generated in Balb/C mice. The antibodies were analyzed using multiplex bead assays on a Luminex platform for HLA-I reactivity. Beads coated with an array of HLA heterodimers admixed with HCs (LABScreen) were used to examine the binding of IgG to different HLA-Ia (31-HLA-A, 50-HLA-B, and 16-HLA-C) and Ib (2-HLA-E, one each of HLA-F and HLA-G) alleles. A striking diversity in the HLA-Ia and/or HLA-Ib reactivity of mAbs was observed. The number of the mAbs reactive to (1) only HLA-E (n = 25); (2) all HLA-Ib isomers (n = 8); (3) HLA-E and HLA-B (n = 5); (4) HLA-E, HLA-B, and HLA-C (n = 30); (5) HLA-E, HLA-A*1101, HLA-B, and HLA-C (n = 83); (6) HLA-E, HLA-A, HLA-B, and HLA-C (n = 54); and (7) HLA-Ib and HLA-Ia (n = 8), in addition to four other minor groups. Monospecificity and polyreactivity were corroborated by HLA-E monospecific and HLA-I shared sequences. The diverse HLA-I reactivity of the mAbs are compared with the pattern of HLA-I reactivity of serum-IgG in non-alloimmunized males, cancer patients, and ESKD patients. The findings unravel the diagnostic potential of the HLA-E monospecific-mAbs and immunomodulatory potentials of IVIg highly mimicking HLA-I polyreactive-mAbs.

Keywords: HLA-Ia, HLA-Ib, HLA-E, heavy chain, open conformers, closed conformers, α1 and α2 helices, β2microglobulin, monomeric, heterodimers, multiplex bead assays, luminex, mean fluorescent intensity (MFI), monospecific, polyreactive, monoclonal IgG antibodies

1. Introduction

HLA class-I expressed on human cell surfaces are heterodimers formed by the non-covalent association of a polymorphic, glycosylated 42-kDa heavy chain (HC) with β2-microglobulin (β2m), a 12-kDa light chain. The HC consists of three helical structures, α1, α2, and α3. HLA-I molecules are polymorphic consisting of several alleles of classical HLA-Ia (HLA-A 6921 alleles, HLA-B 8181 alleles, and HLA-C 6779 alleles) and non-classical HLA-Ib (HLA-E 271 alleles, HLA-F 45 alleles, and HLA-G 88 alleles).

Primarily, intact HLA-I molecules bind to peptides that are derived from proteolytic degradation of cytoplasmic proteins, viruses, and pathogens. The peptide carried in the groove of α1 and α2 domain of the HC interacts with the T cell receptor, and culminates in activation, proliferation, and differentiation of the CD8+ T cells that kill cells that present the specific peptide antigen [1]. After presenting or losing the peptide, HC dissociates from β2m and is released by membrane matrix metalloproteinases (MMPs) [2] from the cell surface into the microenvironment and subsequently the circulation, where it undergoes proteolysis. The HC shedding determines the half-life of HLA-I, which may vary with cells, depending on glycosylation and sialylation [3]. The intact HLA-I molecules are identified with the monoclonal antibody (mAb) W6/32 that recognizes an epitope on β2m (residues 3 and 89) and on the HC residue 121 of all isoforms of HLA-I molecules [4].

In addition, cell surface monomeric (β2m-free HCs) HLA-I variants have been recognized since the 1980s. The HC monomers are found on cells activated by inflammation, infection, trauma, malignancy, cytokines, and chemokines [5,6,7,8,9,10,11,12,13,14,15]. Various functional interactions of these HLA-I monomers have been periodically reviewed [15,16,17,18,19]. The monomeric HLA-I HC functions as a ligand to killer-cell immunoglobulin-like receptors, KIRs (KIE3DS1) on human NK cells and regulates their function [18,19]. These monomers are recognized by the mouse mAb HC-10 but not by mAb W6/32. The epitope of HC-10 is identified between amino acid positions 57 and 62 of the HLA α1 HC; arginine at position 62 (R62) is crucial for recognition [20]. After the shedding of HCs, the amino acid sequences that were masked by β2m are exposed.

The exposure of such cryptic sequences and their fragments is known to elicit an antibody response [21,22,23]. Therefore, one can expect antibodies directed against the HCs of various HLA-I isoforms in normal and healthy individuals. Indeed, natural HLA-Ia Abs occur in normal and healthy human sera and particularly in non-alloimmunized males [24,25,26,27,28,29,30], and they are anticipated to be restricted to native alleles. However, the results of antibody analysis revealed that the antibodies were reactive against several allo-HLA molecules [29,30]. The antibody reactivity of the sera was carried out with LABScreen single antigen bead (SAB) assays using LABScreen beadsets. Examination of the SABs with the mAb W6/32, specific for β2m associated HCs, and the mAb HC-10, confirmed the admixture of HLA-I monomeric HC variants with the intact HLA-I alleles in these beadsets [30,31]. In this context, the primary objective is to understand and elucidate the origin of the allo-HLA reactive Abs occurring in healthy individuals as well as in cancer and transplant patients.

Previously, we have observed that anti-HLA-E mAbs (MEM-E/02, MEM-E/06, MEM-E/07, MEM-E08, and 3D12) not only bind to HLA-E but also to other HLA-I isoforms [32,33], such as the “natural HLA antibodies” observed in the sera of healthy non-alloimmunized males. An inhibition assay with peptides located in the HC, masked by β2m in intact HLA but exposed in β2m-free HCs, was designed to further ascertain the affinity of the mAbs to HLA-E and other HLA-I isoforms. Strikingly, these peptides not only inhibited the mAb binding to HLA-E but also to other HLA-I isomers, namely HLA-A, HLA-B, and HLA-C. These observations suggest that the antibodies reactive to several alleles in normal healthy individuals as well as in those patients waiting for organ transplantation could be those formed against the monomeric variants of HLA found on activated immune cells. If this is true, why are Abs formed against some, but not all, HLA-I alleles of all isomers, although all of them share same sequences such as AYDGKDY?

To address these issues, we have generated monoclonal antibodies to the two recombinant (deglycosylated) heavy chains of HLA-E (HLA-E*01:01 and HLA-E*01:03), which differ in the amino acid at position 107 (HLA-ER107 and HLA-EG107). Here, we study their reactivity against β2m-free HCs of all HLA-Ia alleles. In doing so, we unravel the striking diversity in the mAbs’ specificity, possibly due to differences in the antigenicity and/or immunogenicity of such epitopes.

2. Material and Methods

2.1. Production of Murine Monoclonal Antibodies against HCs of HLA-ER107 and HLA-EG107

Murine monoclonal antibodies were produced following the guidelines approved by the National Research Council’s Committee on Methods of Producing Monoclonal Antibodies [34]. The recombinant polypeptide HCs of HLA-E*01:01 (HLA-ER107) and HLA-E*01:03 (HLA-EG107) (10 mg/mL in 2-(N-morpholino) ethanesulfonic acid (MES) buffer) were obtained from the Immune Monitoring Laboratory, Fred Hutchinson Cancer Research Center (University of Washington, Seattle, WA, USA). Fifty micrograms of the antigen were diluted in 100 μL of PBS (pH 7.4) and were mixed with 100 μL of the adjuvant TiterMax (Sigma–Aldrich, St Louis, MO, USA) before injection into the footpad and peritoneum of mice. Each antigen was injected to 2 mice. Three immunizations were given at about 12-day intervals, with an additional immunization after 12 days for mice receiving HLA-EG107. Only 1 fusion of specific antibody-producing B cells with myeloma tumor cells was carried out for HLA EG107. The clones were cultured in a medium containing RPMI 1640 w/L-glutamine and sodium bicarbonate (Sigma–Aldrich, St. Louis, MO, USA, Cat. No. R8758), 15% fetal calf serum, 0.29 mg/mL L-glutamine/Penn-Strept (Gemini-Bio, MedSupply Partners, Atlanta, GA, USA; Cat. No. 400-110), and 1mM sodium pyruvate (Sigma; Cat. No. S8636). Several clones were also grown using Hybridoma Fusion and Cloning Supplement (HFCS; Roche Applied Science, Indianapolis, IN, USA; Cat. No. 11363735001). Isotypes of all the mAbs were characterized, and no IgM Abs were detected.

Almost all the clones were cryopreserved in liquid nitrogen at the Terasaki Research Institute. The hybridomas of monospecific mAbs (TFL-033, TFL-034, TFL-073, TFL-074, and TFL-0145) and polyreactive mAbs (TFL-006 and TFL-007) were patented [35,36], and deposited with American Type Culture Collection, Patent Depository, 10801, University Blvd. (Manassas, VA, USA).

2.2. Single Antigen Beads Assay with Single-HLA Antigen-Coated Microbeads

To monitor IgG reactivity to HLA-A, HLA-B, HLA-C, HLA-E, HLA-F, and HLA-G, the culture supernatants as well as human sera, were analyzed with SAB assay using dual-laser flow cytometry on a Luminex platform (Luminex xMAP multiplex technology), as described elsewhere [37]. The beadset consists of a panel of color-coded beads on which individual HLA antigens have been covalently bound (xMap assays) to identify antibody specificities. The LABScreen xMap microbeads contain two reporter fluorophores that are proportionally varied to identify them as one of 100 possible bead identifiers. The array of HLA antigens representing various alleles on the beads are listed on the One Lambda web site (www.onelambda.com (accessed on 4 November 2011)) under Ab-detection products/LABScreen Single Ag Product sheet/HLA-Ia combi-LS1A04-Lot 002 or LS1A04-Lot 005 Worksheet Rev-1. The single recombinant HLA-Ia antigens in LS1A04-lot 007 were used for screening the mAbs. The SAB products in every lot include 31 HLA-A, 50 HLA-B, and 16 HLA-C alleles. The beads supplied by the manufacturer have 2 categories of proteins attached to the beads [32,33,37,38,39]: (1) HLA HC polypeptide only and (2) HC in association with β2m. The mean fluorescent intensity (MFI) values were obtained for IgG antibodies reacting to HLA-coated beads each allele of every HLA isomer. The MFI values were corrected against those obtained with negative control values for each allele.

The recombinant HLA-E, HLA-F, and HLA-G HCs (10 mg/mL in MES buffer) were obtained from the Immune Monitoring Laboratory, Fred Hutchinson Cancer Research Center (University of Washington, Seattle, WA, USA) and were coated specially onto beads by the manufacturers (One Lamda Inc., Los Angeles, CA, USA.) of LABScreen beadset. The HLA-E HCs used for immunization were utilized for coating the beads. The recombinant HLA-E, HLA-F, and HLA-G HCs were individually attached by a process of simple chemical coupling to 5.6-micron polystyrene microspheres, which were internally dyed with infrared fluorophores. Positive beads were coated with murine IgG and negative beads were coated with serum albumin (HSA/BSA). In addition, beads treated with PBS were also used as another negative control. For HLA-E, HLA-F, and HLA-G, the control beads (both positive and negative) were added separately. The culture supernatants were diluted 1:50 in PBS at pH 7.2 and 20-μL were added to the 2-μL of antigen-coated microbeads (at 10 to 1 ratio). Secondary fluorescence-labeled anti-mouse polyclonal Abs were diluted 1/100 (anti-mouse IgG (H + L), Cat. No. 115-116-146, protein concentration at 0.5 mg/mL; Jackson ImmunoResearch Laboratories, West Grove, PA, USA). For identifying the isotypes, anti-Fc secondary mouse anti-isotype Abs (human-absorbed anti-mouse IgG1 (Cat. No. 1070-095) obtained from Southern Biotech, Birmingham, AL, USA) were used at concentration 0.5 mg/mL, after 1:100 dilution.

The reporter fluorophore intensity was measured by a Luminex flow cytometer together with the microbead identifiers, and the fluorescence measurement was classified by bead identifier. The trimmed mean was obtained by trimming a percentage off the high and low ends of a distribution and finding the mean of the remaining distribution. The HLA-Ia microbeads have built-in control beads: positive beads that were coated with murine IgG and negative beads that were coated with serum albumin (HSA/BSA). For HLA-E, HLA-F, and HLA-G, the control beads (both positive and negative) were added separately.

Normalized MFI of mAbs were obtained as follows: ((Trimmed MFI—MFI of mAbs treated with PBS alone)—(negative control beads)). This is an essential step because when antibodies were tested on beads with PBS alone, differential MFI values were observed with different HLA molecules [37,38,39]. Each analysis included data obtained from >100 beads and was performed in duplicate. The MFI cut off used for positive HLA reactivity was 500.

2.3. Anti-HLA IgG Purified from the Sera of Non-Alloimmunized Males

The sera used were from non-alloimmunized male volunteers from the Terasaki Foundation Laboratory (TFL). Five milliliters of blood was collected from each volunteer after obtaining informed consent. Fresh sera were used for purification. However, the sera and purified IgG were also frozen and stored at the TFL. The sera were not treated with EDTA. First the sera were tested directly on the SAB assay. To further ascertain the presence of IgG reacting to HLA antigens, IgG was purified from sera using protein-G columns obtained from ThermoFisher Scientific (Rockford, IL, USA). The recombinant protein-G contains two Fc-binding domains and is devoid of albumin and cell surface-binding sites. This was coupled to beaded agarose and ultra-link affinity resins. Protein-G agarose resin binds to all human IgG subclasses but not to IgM, IgD, IgA, or serum albumin. The serum (132 μL) was applied to the protein-G agarose resin after washing the resin twice with PBS, pH 7.2. The serum was mixed well, incubated for 10 min and was passed through the resin. The resin was washed three times with wash buffer (PBS, pH 7.2). The agarose-bound IgG was eluted in three vials using acidic buffer (pH 2.8; 400 μL) and immediately recovered in alkaline buffer (pH 8.5) under centrifugation for a minute, to neutralize the eluates. Either soluble antigen or any other serum factor, such as polypeptides or peptides bound to IgG, would have dissociated at the acid pH used for elution. Three eluates (each 400 μL) were collected and the concentrations of the fractions were determined using the calculations provided in the manual of Eppendorf BioPhotometer (Copyright by 1998 by Eppendorf Netheler—Hinz GmbH, Hamburg, page 87). The calculation of the protein-G fractions was carried out using the calculated factor: C = absorbance at A280—absorbance at A260 to obtain the concentration of the protein (mg/mL). The protein-G eluates (E1, E2, and E3) were tested neat; however, during protein-G elution, the IgGs were diluted 1/10. For this report, we have restricted our observations to data obtained from eluate-2, because the MFIs of E2 were consistently higher than the MFIs of E1 and E3. To detect IgG reactivity to HLA-I alleles in protein G column purified E2 of non-immunized male sera, a multiplex Luminex-based SAB assay was used. The recombinant HLA-Ia antigens in LABScreen beads (1A04-Lot 007) were used for screening. Normalized MFIs of human sera were obtained as follows: ((Trimmed mean MFI—MFI of mAbs treated with PBS alone)—(Negative control bead MFI)—(Negative sera MFI)).

2.4. Sera Anti-HLA IgG Antibodies in Melanoma Patients before and after Autologous Vaccine

In this study, we have used sera collected from patients who participated in the clinical trial, NCI-V01-1646, at the Hoag Cancer Center, Newport Beach, CA, USA. The sera and tumor cells were obtained after institutional review board approval and patient consent [40]. The preparation of the autologous vaccine was described in detail elsewhere [40]. Essentially, tumor cell lines were established from the respective biopsies, expanded (to 150 × 106 cells), and treated with IFNγ for 3 days with (1000 U/mL; ACTIMMUNE, InterMune, Brisbane, CA, USA). The treated cells were harvested, irradiated (at 100 Gray) to arrest growth, and cryopreserved until pulsing with autologous dendritic cells (DCs). For final preparation of the vaccine, tumor cells (TCs) obtained from each patient were incubated (overnight at 37 °C) with autologous DCs at a ratio of 1:1 and cryopreserved into aliquots. Just prior to each vaccination, aliquots of DCs loaded with tumor cells were thawed at 37 °C, washed (2× with AIM-V), mixed with GM-CSF (500 μg/mL) with an average TCs-DCs dose of 1.6 × 107 [±0.8 × 107] cells with 78% viability, and were administered to patients subcutaneously. TCs pulsed with DCs, were given weekly for 3 weeks, then monthly for 5 months. Sera were collected on weeks 0 (before immunization), 4, and 24 (after immunization). This study was restricted to six vaccine recipients whose cell lines showed positivity for anti-HLA-E mAb MEM-E/02 (1/1000). The patient characteristics and HLA reactivity of the sera (diluted 1/10) were described elsewhere [41]. To detect the presence of Abs in the sera that react to HLA-E and HLA-Ia alleles, multiplex Luminex®-based immunoassay (One Lambda, Canoga Park, CA, USA) was used, as described above.

2.5. Sera Anti-HLA IgG Antibodies in HLA-Sensitized End-Stage Kidney Disease Patients Waiting for Transplantation

HLA-I reactivity of the sera of 8 HLA-sensitized end-stage kidney disease (ESKD) patients waiting for donor organs was examined (7 females and 1 male). The causes of ESKD among these patients included diabetes mellitus (DM), hypertensive nephrosclerosis (HN), and lupus erythematosus (LE). Three patients (10DMF73, 12DMF49, and 20HNF64) were possibly sensitized by pregnancy, three (1DMM64, 11DMFDM, and 19HNF45) by transfusion, one (17LEF24) due to prior transplantation, and one (5DM) for unknown reasons. 1DMM64 was transplanted within a year after sample collection for this study. Sera were randomly selected from the Downstate pre-transplant clinic at Sunny Downstate Health Sciences University, University Hospital of Brooklyn (UHB), New York by Prof. Dr. Allen Norin and Dr. Ballabh Das. SAB/DSA study protocols were reviewed and approved by the Downstate Institutional Review Board (IRB), (IRB No. 1232938-3 and No. 341403-1). Sera were examined with LABScreen SABs (Cat. No. 1A04, Lot No. 10). All assays were carried out simultaneously on a single day by one HLA technologist at Terasaki Foundation Laboratory (TFL). For these assays, serum (20 µL) was diluted (1/10) and then incubated with 2 μL of beads for 30 min at room temperature on a shaker. The beads were then washed (3×) with wash buffer. The HLA antigen-IgG binding was assessed with a PE-conjugated secondary-Ab, goat anti—IgH PolyFab (One Lambda Inc., Canoga Park, CA, USA, Cat. No.A82) by incubating the detection-Ab (50 μL at 5 μg/mL) for 30 min at room temperature on a shaker. After washing, the beads were suspended in 1× PBS before acquisition on the Luminex® platform. At least 100 beads were counted for each antigen. The assay includes positive control (coated with human IgG) and negative control (no antigen) beads. We recorded MFI after normalizing the Trimmed mean MFI.

3. Results

Heavy chains (HCs) of two different alleles of HLA-E, which differ in their amino acid residue at position 107, were used for immunization. In one allele (HLA-E*01:01, HLA-ER107), arginine (R) was found in position 107 and in another (HLA-E*01:03, HLA-EG107), arginine was replaced by glycine (G). The monoclonal antibodies generated from these two different alleles were separated as group-R and group-G. Although HCs of each allele were immunized into two mice, only one fusion was carried out for HLA-EG. Therefore, the number of mAbs were higher in HLA-ER than in HLA-EG.

3.1. Classification of Monoclonal Antibodies Formed against HLA-E*01:01 (HLA-ER) and HLA-E*01:03 (HLA-EG)

Table 1 shows the diverse groups (R-I to R-X and GI to GX) and the number of mAbs developed by immunizing HCs of HLA-ER and HLA-EG, and it demonstrates the reactions to different HLA-Ia and HLA-Ib alleles. All mAbs listed in the tables were positive to HLA-E but showed differential reactivity to other alleles, namely 31 HLA-A, 50 HLA-B, 16 HLA-C, and 1 each of HLA-F and HLA-G alleles.

Table 1.

Diverse HLA class-I isoform reactivity of the monoclonal antibodies generated against two alleles of recombinant HLA-E heavy chains.

Immunogen Groups Reactivity of Monoclonal Antibodies with HLA-I Alleles Number of mAbs
HLA-Ia HLA-Ib
HLA-A HLA-B HLA-C HLA-E HLA-F HLA-G
HLA-ER107 R-I Negative Negative Negative Positive Negative Negative 20
R-II Negative Negative Negative Positive Positive Positive 1
R-III Negative B*4006+ Negative Positive Negative Negative 1
R-IV Negative Positive Positive Positive Negative Negative 30
R-V A*1101+ Positive Positive Positive Negative Negative 68
R-VI Positive Positive Positive Positive Negative Negative 29
R-VII A*1101+ Positive Positive Positive Positive Negative 1
R-VIII Positive Positive Positive Positive Negative Positive 2
R-IX A*1101+ Positive Positive Positive Positive Positive 1
R-X Positive Positive Positive Positive Positive Positive 2
HLA-EG107 G-I Negative Negative Negative Positive Negative Negative 5
G-II Negative Negative Negative Positive Positive Positive 7
G-III Negative Negative Negative Positive Positive Negative 1
G-IV Negative Negative Negative Positive Negative Positive 1
G-V Negative Positive Negative Positive Negative Negative 4
G-VI A*1101+ Positive Positive Positive Negative Negative 15
G-VII Positive Positive Positive Positive Negative Negative 25
G-VIII Positive Positive Positive Positive Positive Negative 10
G-IX Positive Positive Positive Positive Negative Positive 16
G-X Positive Positive Positive Positive Positive Positive 4
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Groups R-I (n = 20) and G-I (n = 5) are unique in that they are HLA-E monospecific mAbs. They do not recognize (all MFIs <400) any alleles of other HLA-Ia or HLA-Ib alleles.

Groups R-II (n = 1) and G-II (n = 7) are mAbs specific for HLA-Ib, in that they recognize alleles of HLA-E, HLA-F, and HLA-G but not any of the HLA-Ia alleles.

Group R-III (n = 1) is another unique category of HLA-E mAb that recognizes only B*4006 but not any other HLA-Ib or HLA-Ia alleles.

Group R-VI (n = 29) and G-VII (n = 25) are HLA-E mAbs, that do not recognize any other HLA-Ib loci, such as HLA-F or HLA-G, but recognize all HLA-Ia loci (HLA-A, HLA-B, and HLA-C).

A major category of HLA-E mAbs are those that do not recognize any other HLA-Ib loci but recognize HLA-B, and HLA-C and the A*1101 allele (among all HLA-A alleles) in both R and G groups (R-V: n = 68 and G-VI: n = 15).

The most interesting R and G groups are those anti-HLA-E mAbs that bind to all other HLA-I loci, namely HLA-A, HLA-B, HLA-C, HLA-F, and HLA-G (R-X: n = 2 and G-X: n = 4). These are indeed considered as truly HLA-I polyreactive antibodies.

In addition to these antibodies, there are other groups of HLA-E mAbs that bind to different loci in R and G groups. Furthermore, there was one mAb that tested negative for HLA-E but positive only for A*1101 (unpublished).

3.2. The Monospecific mAbs Formed against HLA-E*01:01 and HLA-E*01:03

Table 2 illustrates 20 HLA-E monospecific mAbs developed after immunizing two different Balb/c mice with recombinant HLA-ER107 and 5 HLA-E monospecific mAbs after immunizing with recombinant HLA-EG107. Isotypes of these mAbs were IgG1 in 20/25 mAbs and IgG2a in 3 mAbs out of 25. Isotypes of the two mAbs could not be tested. The MFIs ranged from 10K to 22K for 13 of 25 mAbs. To determine the possible binding sites of these monospecific mAbs, we compared the amino acid sequences of the two HLA-E allelic sequences with the amino acid sequences of 504 HLA-A, 844 HLA-B, and 283 HLA-C alleles. The amino acid sequences of HLA-E, HLA-F, and HLA-G are compared in Figure 1. The HLA-E specific sequences in the α1 and α2 helical domains are shown within the red boxes. Figure 2 provides additional proof for HLA-E monospecificity obtained by comparing the profiles of the amino acid sequences of 30 alleles of HLA-A, 58 alleles of HLA-B, and 15 alleles of HLA-C coated on the microbeads of LABScreen beadsets.

Table 2.

HLA-E monospecific mAbs identified with their hybridoma and subclass, produced after immunizing mice with recombinant (non-glycosylated); HLA-ER107 and HLA-EG107 heavy chains.

Groups Number of mAbs mAb Nomenclature Hybridoma ID Antigen (Heavy Chain only) Tested on Beads Subclass HLA-E Reactivity in MFI
(Shorter Version of MFI)		 HLA-F Reactivity (MFI) HLA-G Reactivity (MFI) HLA-A Reactivity (MFI) HLA-B Reactivity (MFI) HLA-C Reactivity (MFI)
R-I 1 TFL-147 FA5746-30F1C8 HLA-ER IgG1 22,829 (22+) 0 0 0 0 0
2 TFL-148 FA5746-30F1D10 HLA-ER IgG1 16,644 (16+) 0 0 0 0 0
3 TFL-110 FT5742-14B1G2 HLA-ER NT 16,226 (16) 0 0 0 0 0
4 TFL-034 FT5742-8C6F6 HLA-ER IgG1 13,272 (13) 0 0 0 0 0
5 TFL-125 FA5746-14A5E9 HLA-ER IgG1 13,204 (13) 0 0 0 0 0
6 TFL-033 FT5742-8C6F4 HLA-ER IgG1 13,025 (13) 0 0 0 0 0
7 TFL-126 FA5746-14A5F12 HLA-ER IgG1 12,397 (12) 0 0 0 0 0
8 TFL-074 FT5742-20E2E9 HLA-ER IgG1 10,269 (10) 0 0 0 0 0
9 TFL-073 FT5742-20E2G7 HLA-ER IgG1 10,088 (10) 0 0 0 0 0
10 TFL-144 FA5746-28D10C6 HLA-ER IgG1 9180 (9) 0 0 0 0 0
11 TFL-041 FT5742-11B4A2 HLA-ER IgG1 8914 (8+) 0 0 0 0 0
12 TFL-043 FT5742-11B7F12 HLA-ER IgG1 8448 (8) 0 0 0 0 0
13 TFL-145 FA5746-29B1C10 HLA-ER IgG1 7622 (7+) 0 0 0 0 0
14 TFL-042 FT5742-11B4A6 HLA-ER IgG1 7452 (7) 0 0 0 0 0
15 TFL-182 FA5746-32D10D2 HLA-ER IgG1 7280 (7) 0 0 0 0 0
16 TFL-001 FT5742-1A1E2 HLA-ER IgG2a 4691 (4+) 0 0 0 0 0
17 TFL-081 FT5742-23F8C12 HLA-ER IgG1 4261 (4) 0 0 0 0 0
18 TFL-016 FT5742-4F8F12 HLA-ER IgG2a 1349 (1) 0 0 0 0 0
19 TFL-047 FT5742-12B4E10 HLA-ER NK 1044 (1) 0 0 0 0 0
20 TFL-013 FT5742-4B3D8 HLA-ER IgG2a 943 (0+) 0 0 0 0 0
G-I 1 TFL-185 FA5850-1E1E6 HLA-EG IgG1 19,644 (19+) 0 0 0 0 0
2 TFL-184 FA5850-1E1C2 HLA-EG IgG1 19,227 (19) 0 0 0 0 0
3 TFL-186 FA5850-1E1C3 HLA-EG IgG1 19,075 (19) 0 0 0 0 0
4 TFL-226 FA5850-9C3B4 HLA-EG HLA-ER IgG1 18,865 (18+)
18,979 (18+)		 0 0 0 0 0
5 TFL-254 FA5850-14C7F1 HLA-EG HLA-ER IgG1 1604 (1+) 1972 (1+) 0 0 0 0 0
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For HLA-A, HLA-B, and HLA-C LABScreen beatsets were tested, which carry both intact HLA (closed conformers) β2m-free heavy chain of HLA (open conformers). For HLA-E, HLA-F, and HLA-G fresh beads coated with β2m-free heavy chains (open conformers) were tested.

Figure 1.

Figure 1

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Comparative profile of the amino acid sequences of HLA-E, HLA-G, and HLA-F, and a partial profile of the sequences of mice equivalent of HLA-E, known as Qa1b. The amino acid sequences of HLA-E are compared with the amino acid sequences of HLA-G and HLA-F, and also with 504 HLA-A, 844 HLA-B, and 283 HLA-C alleles, to determine the HLA-E specific sequences. The HLA-E specific sequences in α1 and α2 helical domains are shown within the red boxes. HLA-Ib shared epitopes area are shown in black and blue boxes. Sequences in the blue boxes are shared with all HLA-Ia isoforms, while those in black boxes are shared with most, if not all, HLA-Ia alleles.

Figure 2.

Figure 2

Figure 2

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Comparative profile of the amino acid sequences of HLA-Ia alleles (HLA-A, HLA-B, and HLA-C) and HLA-Ib (HLA-E, HLA-F, and HLA-G) isoforms: HLA-Ia and HLA-Ib shared sequences and HLA-Ib and HLA-Band HLA-C shared sequences coated on the microbeads of LABScreen beadsets. (A) HLA-E,-F,-G compared with 30 alleles HLA-A coated on the LABScreen beadset; (B) 58 alleles HLA-B coated on the LABScreen beadset; (C) 15 alleles HLA-C coated on the LABScreen beadset; and (D) HLA-E amino acid sequences shared with HLA-Ia and HLA-Ib alleles.

To further confirm the monospecificity, we carried out dosimetric inhibition of purified culture supernatants of TFL-033 with two HLA-E-restricted peptides, 65RSARDTA71 and 143SEQKSNDASE152, at concentrations ranging from 4.4 to 0.27 mg/well. Although both peptides showed inhibition, the α2 helical peptide SEQKSNDASE showed better dosimetric inhibition than the other peptide [37]. Figure 3 illustrates the conformational orientation of the HLA-E mAb specific epitopes on the α1 and α2 helices of HLA-E. It is important to note that the mAbs do not recognize linear epitopes but recognize conformationally altered epitopes. The yellow letters in the figures represent the exposed amino acids on the epitope, while the amino acids in the white letters represent the masked ones.

Figure 3.

Figure 3

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(A,B) represent the two different conformational orientations of HLA-E specific amino acid sequences exposed on α1 and α2 helices of HLA-E. Note a brown colored peptide sequence in the groove. The monospecific epitopes are on either side of the grove. These sequences are recognized by TFL-033 as evidenced by the binding inhibition of the mAb to the beadsets coated with HLA-E after incubation with the peptide sequences, synthesized and obtained commercially (GenScript Corporation, Piscataway, NJ, USA). It is important to note that the mAb recognizes the sequences, which are not linear but folded and exposing few amino acids (colored yellow) of the epitope for immune recognition; this is an important characteristics of HLA-E mAbs.

Indeed, this is an important characteristic feature of monoclonal antibodies recognizing quaternary structures. The specific amino acids at the center of an epitope that are recognized by an antibody are designated as eplets, and usually consist of one to several amino acids. Another noteworthy finding of the HLA-E sequences is that there are several HLA-E specific sequences in addition to 65RSARDTA71 and 143SEQKSNDASE152, as shown in red boxes in Figure 1. These sequences may serve as the epitopes for other HLA-E monospecific mAbs.

3.3. The HLA-Ib Specific but HLA-Ia Non-Reactive mAbs

Table 3 documents ten HLA-Ib reactive mAbs that are totally non-reactive to HLA-Ia, of which one (TFL-050) was produced by HLA-ER (Group R-II) and the other nine mAbs were produced by HLA-EG. Seven of the nine mAbs were binding to HLA-E, HLA-F and HLA-G (Group G-II). Interestingly the MFI of mAbs reactive to HLA-G are almost equal to or higher than those produced with HLA-E, although the immunogen is HLA-EG. Two HLA-E reactive mAbs reacted with either HLA-F (Group G-III) or HLA-G (Group G-IV) at low levels. Figure 1 illustrates some of the amino acid sequences that are common for HLA-Ib isoforms, although these sequences are also found in several but not in all HLA-I alleles. Since we could not decipher specific linear sequences shared among HLA-Ib isoforms, it is inferred that the HLA-Ib specific mAbs may recognize the sequences (in the black boxes) folded in certain unique conformations characteristic for HLA-Ib. Furthermore, the only difference in the amino acid sequences between HLA-EG107 and HLA-ER107 is the presence glycine in the former, and arginine, at position 107 in the latter. Of the total mAbs obtained after immunizing a single mouse with HLA-EG107, nine mAbs are HLA-Ib reactive, whereas only one mAb is reacting to HLA-Ib of the total mAbs obtained after immunizing two mice with HLA-ER107, suggesting that the presence of arginine at 107 in HLA-E may impact the conformational orientation of the HLA-E.

Table 3.

HLA-Ib specific mAbs are non-reactive to HLA-Ia molecules.

Groups Subgroups Number of mAbs mAb Nomenclature Hybridoma ID Antigen (Heavy Chain only) Tested on Beads Subclass HLA-E Reactivity in MFI HLA-F Reactivity (MFI) HLA-G Reactivity (MFI) HLA-A Reactivity (MFI) HLA-B Reactivity (MFI) HLA-C Reactivity (MFI)
R-II 1 TFL-050 FT5742-12B6F3 HLA-ER IgG2b 4876 (4+) 3292 (3) 2683 (2+) 0 0 0
G-II 1 TFL-209 FA5850-5C4F3 HLA-EG
HLA-ER 		IgG1 21,532 (21+)
21,452 (21)		 8744 (8+) 20,303 (20) 0 0 0
2 TFL-208 FA5850-5C4E2 HLA-EG
HLA-ER 		IgG1 21,294 (21)
21,030 (21)		 8605 (8+) 20,562 (20+) 0 0 0
3 TFL-223 FA5850-8D3A4 HLA-EG
HLA-ER 		IgG1 18,447 (18)
21,086 (21)		 8587 (8+) 20,587 (20+) 0 0 0
4 TFL-164 FT5765-1C1B6 HLA-EG IgG2b 15,370 (15) 8230 (8) 24,765 (24+) 0 0 0
5 TFL-165 FT5765-1C1G5 HLA-EG IgG2b 15,192 (15) 8254 (8) 25,812 (25+) 0 0 0
6 TFL-162 FT5765-1C1B4 HLA-EG IgG2b 14,667 (14+) 9015 (9) 25,085 (25) 0 0 0
7 TFL-161 FT5765-1C1B1 HLA-EG IgG2b 14,444 (14) 8607 (8+) 24,714 (24+) 0 0 0
G-III 1 TFL-228 FA5850-9C3B3 HLA-EG IgG1 19,581 (19+)
19,919 (19+)		 1134 (1) 0 0 0 0
G-IV 1 TFL-191 FA5850-2C8C10 HLA-EG NK 1000 (1) 0 1016 (1) 0 0 0
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Note: HLA-A, HLA-B, and HLA-C LABScreen beatsets were tested, which carry both intact HLA (closed conformers) and (open conformers). NK: not known; for HLA-E, HLA-F, and HLA-G, fresh beads coated with β2m-free heavy chains (open conformers) were tested.

3.4. Expanding Diversity of the HLA-Ia Reactivities of HLA-E*01:01 (HLA-ER) mAbs

3.4.1. HLA-B*4006 But Not HLA-A, HLA-C, HLA-F, and HLA-G Reactive (Group R-III)

Group R-III represented by mAb TFL-114 in Table 4 illustrates a unique feature of allelic specificity, in that it reacted only with B*4006, in addition to recognizing HLA-E.

Table 4.

HLA-ER mAbs: HLA-F, HLA-G, and HLA-A non-reactive but reactive to HLA-B and HLA-C alleles.

MFI Expressed in Thousands
Groups Subgroups number of mAbs mAb Nomenclature Subclass HLA-E Reactivity (MFI) HLA-F Reactivity (MFI) HLA-G Reactivity (MFI) HLA-A Reactivity (MFI) HLA-B Reactivity HLA-C Reactivity
B*0801 B*1301 B*1302 B*1401 B*1501 B*1502 B*1511 B*1512 B*1513 B*1516 B*3501 B*3701 B*4006 B*4601 B*5701 B*5703 B*5801 C*0202 C.*0401 C*0501 C*0602 C*0702 C*0801 C*1802
R-III 1 TFL-114 IgG2a 14+ 1
R-IV [A] 1 TFL-004 IgG2a 17+ 1 1
2 TFL-005 IgG2a 17 1 1
3 TFL-019 IgG1 13+ 1+ 1+
4 TFL-020 IgG1 12+ 1 1+
5 TFL-099 IgG2a 14 1+ 1+
6 TFL-108 IgG2a 11 1 1
7 TFL-128 IgG3 13 1 1
8 TFL-146 IgG1 13 1 1
9 TFL-084 IgG2a 16 2 1+
10 TFL-028 IgG1 12+ 2 2
[B] 11 TFL-116 IgG1 8+ 1+ 1 2
12 TFL-156 IgG1 12+ 2 1 2+
13 TFL-029 IgG1 13+ 2+ 1 2+
14 TFL-030 IgG2a 15 2+ 1 2+
15 TFL-060 IgG2a 18+ 2+ 1 3
16 TFL-154 IgG2b 19+ 3 1 3
[C] 17 TFL-158 IgG2b 19 1 4 1+ 1 6
18 TFL-059 IgG2a 19+ 1 1 3+ 1+ 4
19 TFL-079 IgG1 11+ 1 3+ 1 1+ 1 4
20 TFL-093 IgG1 13+ 1+ 1 1 1
21 TFL-143 IgG2b 20+ 1 1 4+ 1 1 5
[D] 22 TFL-090 IgG1 12 1 3 1 2 1 4
23 TFL-052 IgG2b 10+ 1+ 1 2+ 1 1+ 1 3+
24 TFL-062 IgG1 15 1 1 3+ 1 2 1 4
25 TFL-076 IgG2b 20+ 1 1 4 1 1+ 1 4+
26 TFL-076 IgG2b 20+ 1 1 4 1 1+ 1 4+
27 TFL-091 IgG1 16+ 1 1 4 1 1+ 1 5
28 TFL-159 IgG2b 19+ 1+ 1 5 1 2 1 1+ 7
[E] 29 TFL-155 IgG2b 19 8+ 7+ 9 9+ 17 4+ 15 16+ 2+ 16+ 6+ 8 3
30 TFL-094 IgG2b 18+ 12+ 8 9 10+ 18 5 16+ 17+ 2 17 6 8 2+
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HLA-EG107 not tested.

3.4.2. HLA-B and HLA-C Reactive But Not HLA-A, HLA-F, or HLA-G Reactive (Group R-IV)

Table 4 also illustrates the evolving diversity of the anti-HLA-ER mAbs represented by the subgroups of Group R-IV. This group is HLA-A, HLA-F, and HLA-G non-reactive. Subgroups A, B, C, D, and E in Table 4 illustrate two important aspects of the mAbs. They are (1) the number of alleles recognized by the mAbs progressively increases from subgroup A to E; and similarly, (2) the MFI of these mAbs for each of the alleles also progressively increases from subgroup A to E. Indeed, 27 out of 28 mAbs (except one) recognize B*4006 positively.

Figure 2 sheds light on why Group R-IV is not reactive to HLA-A. The figure shows several shared amino acid sequences among HLA-E, HLA-B, and HLA-C (green band). Subgroups C, D, and E may be recognizing 137DTAAQI142 as evidenced by the alleles carrying this sequence. The sequence 137DTAAQI142 is found in HLA-E/-G/-B and –C, whereas in HLA-A, it is 137DMAAQI142 (Figure 2). As shown in Figure 4, 137DTAAQI142 is evidently a cryptic epitope masked by β2m. Further careful examination of the sequence will reveal that even in the absence of β2M, DTA and DTM are exposed in addition to QI. Therefore, we infer that the R-IV-subgroups, more importantly subgroup E, recognizes 137DTAAQI142. Peptide inhibition of binding of every one of the R-IV subgroup E mAbs with the beadset may validate the inference.

Figure 4.

Figure 4

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(A) The conformational orientation of HLA-E clarifying the location of TFL-006/TFL-007 binding domain (in yellow) in all HLA class I molecule. (B) Figure illustrates the steric hinderence caused by β2-microglobulin (blue); therefore 117AYDGKDY123, is a cryptic epitope not accessible in intact HLA molecules (closed conformers); (C) Figure illustrates exposure of 17AYDGKDY123 on β2-m-free heavy chains of HLA (open conformers).

Since HLA-E mAbs may recognize non-linear sequences, it is possible that the anti-HLA-E mAbs recognize unique conformations of the shared amino acid sequences of B*4006 (Figure 5). In support of this suggestion, Figure 6 illustrates the proportional variations in the MFI of the mAbs reacting to HLA-E and B*4006. The ten mAbs in subgroup A of Group R-IV reveal binding affinity for B*4006 and C*1802. Similarly, six mAbs in subgroup B of the Group R-IV show affinity for C*0501 in addition to recognizing B*4006 and C*1802. The 14 mAbs in subgroups C, D, and E show expanded affinity for other alleles of HLA-B and HLA-C isoforms in addition to the stronger binding affinity for B*4006 and C*1802.

Figure 5.

Figure 5

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The amino acid sequences of HLA-E compared with the unique B (B*4006, B*1511) or C (C*1802) alleles recognized by a few HLA-ER or HLA-EG mAbs. The asterix in the figure illustrates amino acids, and sequences shared between HLA-E and B*4006 or B*1511 or C*1802.

Figure 6.

Figure 6

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MFI of mAbs reacting to HLA-ER and B*4006, a major HLA-Ia antigen recognized by almost all mAbs.

3.4.3. HLA-B and HLA-C Reactive but HLA-F, HLA-G Non-Reactive While Having Unique Affinity for HLA-A*1101 (Group R-V)

The expanding diversity of Group R-IV continues into Group V mAbs, as evidenced by the subgroups A, B, C, D, E, F, and G (Table 5). Group R-V mAbs in general recognized a greater number of alleles of HLA-B and HLA-C compared to Group IV. Most importantly, there was no noteworthy decrease in MFIs of HLA-E reactivity despite the expansion into additional allelic recognition. Subgroups A, B, C, D, E, F, and G in Table 5 also illustrate the two important aspects of the mAbs, namely (1) the number of alleles recognized by the mAbs progressively increases from subgroup A to E, and (2) similarly the MFI of these mAbs for each of the alleles also progressively increased from subgroup A to E. The most striking feature of these subgroups is that they also recognized exclusively HLA-A*1101 but not any other HLA-A alleles. Indeed, all the 68 mAbs recognized B*4006 positively, with a progressive increase in MFI from subgroup A to G.

Table 5.

HLA-ER mAbs: HLA-F and HLA-G non-reactive but reactive to HLA-A*1101, HLA-B, and HLA-C alleles.

MFI expressed in Thousands
Groups Subgroups number of mAbs mAb Nomenclature Subclass HLA-E Reactivity (MFI) HLA-F Reactivity (MFI) HLA-G Reactivity (MFI) HLA-A*1101 Reactivity (MFI) HLA-B Reactivity HLA-C Reactivity
B*1301 B*1302 B*1401 B*1501 B*1502 B*1511 B*1512 B*1513 B*1516 B*1801 B*3501 B*3701 B*3901 B*4001 B*4002 B*4006 B*4101 B*4403 B*4501 B*4601 B*4701 B*5301 B*5401 B*5701 B*5703 B*5801 B*7801 B*8201 C*0202 C*0303 C*0304 C*0501 C*0602 C*0801 C*1402 C*1502 C*1601 C*1701 C*1802
R-V [A] 1 TFL-035 IgG1 11 1 1 1 3+ 1 2 1+ 4+
2 TFL-064 IgG2b 21 1 1 1 4+ 1 1 4
3 TFL-134 IgG1 15+ 1 1+ 1 4 1 1+ 1 5
4 TFL-075 IgG1 16 1 1+ 1 4+ 1 2 1 5
5 TFL-011 IgG2a 17 1 1 1+ 4 1 2 1 1
6 TFL-061 IgG1 15 1 1+ 1+ 4+ 1+ 2+ 1+ 5
7 TFL-077 IgG2b 21+ 1 1+ 1+ 6 1+ 2+ 1 6
[B] 8 TFL-036 IgG1 11+ 1 1 1 4 1 1 2 1+ 4+
9 TFL-078 IgG1 12+ 1 1 1 4 1 1 2+ 1+ 5
10 TFL-096 IgG1 12+ 1 1+ 1 3+ 1 1 2 1+ 4
11 TFL-012 IgG2a 17+ 1 1 1+ 4+ 1 1+ 2 1+ 4+
12 TFL-045 IgG2a 16+ 1+ 1 1 5 1 1+ 2+ 1+ 5
13 TFL-160 IgG3 15+ 1+ 1+ 1+ 5+ 1 1+ 2 1 5+
14 TFL-105 IgG2a 19 1 2 1+ 5 1 1+ 2+ 1+ 5+
15 TFL-120 IgG1 16 1+ 2 2 5+ 1 1+ 2+ 1+ 6
16 TFL-087 IgG2b 21+ 1+ 2 2 6 1 1+ 3+ 2 7+
[C] 17 TFL-106 IgG2a 19 1+ 2 2 5+ 1 1 1 2 3 2 6
18 TFL-032 IgG2b 21+ 1+ 2+ 2 6 1 1 1 2 3+ 2 7
19 TFL-068 IgG2a 20+ 1+ 2+ 2 6+ 1 1 1 2 3+ 2 7+
20 TFL-088 IgG2b 21+ 1+ 2+ 2 7 1 1 1+ 2 4 2+ 8+
21 TFL-065 IgG1 18 2 3 2+ 7 1 1 1+ 2 3+ 2 8
22 TFL-149 IgG2b 22 2+ 3 2+ 1 8 1 1 1+ 2+ 3+ 2+ 4
23 TFL-150 IgG2b 22+ 2 2+ 2+ 1 7+ 1 1 1+ 2 3 2 8
24 TFL-066 IgG1 16+ 2 3 2+ 1 7+ 1 1+ 1+ 2+ 4 2+ 8+
25 TFL-067 IgG2a 20 1+ 2+ 2 6+ 7 1 1 1 2 3+ 2 7+
[D] 26 TFL-130 IgG1 16 1+ 2+ 1 1 2 5+ 1 1 2 2+ 1+ 6+
27 TFL-117 IgG2b 22 2 3 1 1 2+ 1 8 1 1 1+ 2+ 3+ 2 8+
28 TFL-151 IgG1 16+ 1+ 2+ 2 5+ 1 1 1+ 2+ 1+ 2 6+
29 TFL-136 IgG2b 20 1 2 1+ 1 6 1 1 3 1+ 2 8+
30 TFL-135 IgG1 16 2 3 2 1 6 1 1+ 1+ 2 3 1+ 2+ 1 1 7
31 TFL-152 IgG2b 20 1 2 1+ 1 6 1 1 3 2 2 1 1 8+
32 TFL-100 IgG1 16+ 2+ 2+ 1 3 1 1 7+ 1 1+ 2 2+ 3+ 2+ 7
33 TFL-140 IgG2b 22+ 2+ 3+ 1 3 1 8+ 1 1+ 1+ 3 4+ 2+ 9+
34 TFL-139 IgG2b 22+ 3 3+ 1 3+ 1 1 9 1 1+ 2 3 4+ 3 10
35 TFL-124 IgG1 16 2+ 3 1 2+ 1 6+ 1 1 1+ 1+ 2+ 3+ 2 2+ 1 1 7+
36 TFL-009 IgG2a 17+ 2 2 1 1 2+ 1 1 6 1 1+ 1+ 1+ 2+ 1 4 2+ 7+
37 TFL-053 IgG2a 20 2 3 1 2+ 1 1 7+ 1 1+ 1+ 1+ 2+ 4 2+ 8
38 TFL-054 IgG2a 20+ 2 3+ 1 3 1 1 7+ 1 1+ 1+ 1+ 2+ 4+ 3 8
39 TFL-089 IgG2a 20+ 2 3 1 2+ 1 1 7+ 1 1+ 1+ 2 2+ 4+ 3 8+
40 TFL-115 IgG2a 19 2 3 1 2+ 1 1 7 1 1+ 1+ 1+ 2+ 4 2+ 8
[E] 41 TFL-121 IgG1 20+ 2+ 3 1 1 1 1 3 1 8 1 1+ 1+ 2+ 4 2+ 8+
42 TFL-002 IgG2a 19+ 2+ 2+ 1 1 1 3 1 1 7 1 1+ 2 2 3 1 4 2+ 7+
43 TFL-003 IgG2a 19 2+ 2+ 1 1 3 1 1 7+ 1 1+ 2 2 3 1 4 3 7+
44 TFL-021 IgG1 18 3 4 1 1 1 3+ 1 1+ 8+ 1 1+ 2 2+ 1 3 1 5 3+ 9
45 TFL-022 IgG1 18 3 4 1 1 1 3 1 1 8 1 1+ 2 2 3 1 4+ 3 8+
46 TFL-023 IgG2a 21 2+ 3+ 1 1 1 3 1 1 8+ 1 1+ 2 2 1 3 1 5 3+ 9
47 TFL-024 IgG2a 20+ 2+ 4 1 1 1 3 1 1 8 1 1+ 2 2 3 1 5 3 0
48 TFL-031 IgG1 18+ 3 3 1 1 1 4 1 1 9 1 1+ 2 2+ 3+ 1 5 3 9+
49 TFL-123 IgG1 16+ 2+ 3 1 1 3 1 1 7 1 1+ 1+ 2 3 1 4 3 8
50 TFL-122 IgG1 17 3 3+ 1 1 3 1 1 7+ 1 1+ 3 2 3 1 4 3 8+
51 TFL-131 IgG2a 20+ 3 4 1 1 1 1 3+ 1 1 1+ 7+ 1+ 2 2 2+ 1 3+ 1 1 5 3 8
52 TFL-132 IgG2a 20 2+ 3+ 1 1 1 3 1 1 6+ 1 1+ 1+ 2 1 3 1 4+ 3 7+
[F] 53 TFL-138 IgG2a 22 3 1 4 1+ 2+ 2 1+ 1 4 1 1+ 9+ 1 1+ 2 1 2+ 3+ 1 5 3+ 10+
54 TFL-083 IgG1 17+ 2+ 1+ 4 2 4+ 3+ 3 1 3+ 1 1 8 1 1+ 2 2+ 2 3 1 4+ 3 9
55 TFL-157 IgG1 16 2+ 1 3 1+ 3+ 2+ 2 1 3 1 1 7 1 1 1+ 1+ 2 2+ 3+ 2+ 7+
56 TFL-092 IgG1 17+ 2+ 1+ 3+ 2 4+ 3+ 2+ 1 3 1 1 1 7+ 1 1 1+ 2 2 2+ 4 2+ 8
57 TFL-127 IgG1 16+ 3 1+ 3+ 2 4+ 3+ 3 1 3+ 1 1+ 7+ 1 1+ 2 2+ 2 1 3 1 4+ 3 8+
58 TFL-137 IgG2b 22 3+ 1 4+ 1+ 3 2+ 2 1 4 1 1+ 9+ 1 1+ 2 1+ 2+ 1 3+ 1 5 3+ 10+
59 TFL-040 IgG2a 19 3+ 1 3+ 1 1 1+ 4 1+ 1+ 8+ 1+ 2 2+ 1 3 1 4 1 1 1 5 3+ 9
60 TFL-080 IgG1 14 2+ 5 1 1+ 1 2 3+ 1 1+ 1 1+ 8 1+ 2 2+ 1 1 2+ 1 3 1 1 1 1 1 6 4+ 1 10
61 TFL-026 IgG1 17+ 3 4 1 1+ 1 1+ 3 1+ 1 1+ 7+ 1+ 2 2+ 1 1 2+ 1 3 1 1 1 5 3+ 1 1 8
[G] 62 TFL-119 IgG2a 18+ 1+ 5+ 5 2+ 7 6+ 12+ 3+ 11+ 9+ 2 5+ 1 1 9+ 1 3+ 5+ 2 3 2 7
63 TFL-142 IgG2b 22+ 3 9+ 8+ 4+ 10+ 11 17+ 6 15+ 15 1 3+ 1 1 9 1 1+ 2 13+ 2 7 9 3+ 1 5 3+ 10
64 TFL-153 IgG2b 22 2+ 10 8+ 4 10+ 11 18 6 16 14+ 1 3+ 1 1 8 1 1+ 1+ 14 2 7 9 3 1 5 3 10
65 TFL-118 IgG2a 20+ 3+ 10+ 10 5 12+ 12+ 20 8 17+ 17+ 1+ 4 1+ 1+ 9 1 2 2 16 2+ 1 8+ 10+ 3+ 1 1 5+ 3+ 10+
66 TFL-133 IgG2a 22 3+ 11+ 10+ 5 12+ 13 20+ 8 17+ 17+ 1+ 4+ 1+ 1 1+ 10 1+ 2 2+ 16 3 1 9 10+ 4 1 1 6 4 11
67 TFL-141 IgG2b 22+ 3+ 11+ 11 5+ 13 13+ 21 8 18+ 18 1+ 4+ 1+ 1 1+ 10+ 1+ 2 2+ 17 3 1 9 11+ 4 1 1 6+ 4 12
68 TFL-095 IgG2b 22+ 4+ 13 13+ 6+ 17 16+ 24 12 21+ 20+ 2 5 1 2 1 2 11+ 1+ 2+ 3 21 3+ 1 12 13+ 5 1+ 1 1 7+ 5 13
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3.4.4. HLA-Ia Reactive but HLA-F, and HLA-G Non-Reactive (Group R-VI)

The diversity of HLA-ER binding mAbs further expands in Group R-VI, to recognize all of HLA-Ia isoforms (HLA-A, HLA-B, and HLA-C), while not recognizing HLA-F and HLA-G (Table 6). We could identify five subgroups of Group VI. Subgroup A recognized A*3303 in addition to A*1101. In subgroup B, not only the MFIs of mAbs recognizing A*1101 increased, but also there was recognition of A*3601 and A*3401, and much more of A-alleles in other subgroups under Group VI. Group VI in general recognized a greater number of alleles of HLA-B and HLA-C.

Table 6.

Evolving HLA-ER mAbs: HLA-F, HLA-G non-reactive but reactive to HLA-A, HLA-B, and HLA-C alleles.

MFI Expressed in Thousands
Groups R-VI
Subgroups [A] [B] [C] [E] [F]
Number of mAbs 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29
mAb Nomenclature TFL-015 TFL-027 TFL-039 TFL-111 TFL-010 TFL-014 TFL-082 TFL-102 TFL-101 TFL-072 TFL-086 TFL-113 TFL-085 TFL-008 TFL-038 TFL-057 TFL-098 TFL-025 TFL-112 TFL-037 TFL-071 TFL-097 TFL-048 TFL-058 TFL-017 TFL-018 TFL-070 TFL-051 TFL-069
Subclass IgG2a IgG1 IgG2a IgG2a IgG1 IgG2a IgG2a IgG2b IgG2b NT IgG3 IgG2b IgG3 IgG1 IgG2b IgG1 IgG1 IgG1 IgG2b IgG2b IgG2b IgG2a IgG1 IgG1 IgG1 IgG1 IgG2b IgG2b IgG2b
HLA-E Reactivity 19+ 17+ 20 18+ 18+ 19+ 18+ 22 21+ 15+ 17 16 16 19 24 17 17+ 17 23 24 23+ 21 17+ 17+ 17+ 17+ 23 23+ 22+
HLA-F Reactivity
HLA-G Reactivity
HLA-A Reactivity A*1101 3+ 3+ 4 3+ 4 4 4 5 5 3 4 3 4+ 5+ 6+ 3+ 4+ 5+ 7 7 8 7 4 4+ 4+ 4+ 8+ 8+ 9
A*2402 1 1 1+ 2 1+ 1 1+ 2 1 1 1 2 2 2
A*2403 1 1+ 1 1 1 1+ 1 1 1 1+ 1+ 1+
A*2601 1 1 1
A*2901 1 1 1 1 1 1 1+ 1 1+ 2 1 1+ 1+ 1+ 2 2 2
A*3001 1
A*3002 1 1 1 1 1+ 1+ 1+
A*3201 1 1 1 1 1 1 1+ 1+ 1+
A*3301 1 1 1 1 1 1 1 1+ 1+
A*3303 1 1 1 1 1 1 1 1 1 1 1 1+ 1+ 1+ 1+ 1+ 1 1+ 2 2 2+ 3 2 2 2+ 2 3+ 3+ 3+
A*3401 1 1 1 1 1 1 1 1 1+ 1 1+ 1+ 1+ 2 2 2 2 2 2+ 2+ 3
A*3601 1 1 1 1 1 1 1 1 1 1 1 1 1+ 1+ 2 2+ 1 1+ 1+ 1+ 2+ 2+ 3
A*4301 1 1 1 1 1 1+ 1+
A*6601 1
A*6802 1 1 1
A*8001 1 1 1
HLA-C Reactivity C*0102 2+ 1 1 1 2 1+ 1+ 1+ 1 1+ 1+
C*0202 1+ 1+ 1 1 1 1+ 1 1 1+ 2 2+ 2 1+ 2+ 3 1 2 2+ 2+ 3 3 4+ 3+ 3+ 3+ 4 4 4+
C*0302 2 1 1+ 1 1 1 2 1 1 1 1+ 1+ 3 2 2+ 2 2 2 2+
C*0303 1 1 1 1 1 1+ 1+ 2 1+ 1+ 1+ 2+ 1 1+ 2+ 2 2+ 3 3+ 3 3 3 3+ 3+ 4
C*0304 1 1 1+ 1+ 1+ 1 1+ 2+ 1 1 1+ 1+ 2 2+ 3 3 3 2+ 3 3 3+
C*0401 1 1+ 1 1 1 1
C*0501 5+ 5+ 5+ 5 5+ 5+ 6 7 7 6+ 6 6+ 6+ 7+ 9+ 7 6 7+ 10 10 10+ 9 9+ 8 7+ 7+ 11 11 11+
C*0602 1 1+ 1 1 1+ 1 1 1 1+ 5 2 2+ 2 2 2 2
C*0702 1 1 1 1 3+ 1+ 1+ 1+ 1 1 1+
C*0801 3+ 4 4 3+ 4 4 4 5 4+ 5 5 4+ 5 5+ 7 6 4+ 6 7+ 7+ 8 7+ 7 7 6 6 9 9+ 9+
C*1203 2 1 1 2 1 1+ 1+ 1 1 1
C*1402 1 1 1 1 1 1 1 1 1 1+ 2 1+ 1+ 1+ 2+ 1 1+ 2 2 2 3 4 3 3 3 3+ 3+ 4
C*1502 1 1 1+ 1 1 1 1+ 1 1+ 1+ 1+ 2 2+ 2 2+ 2 2+ 2+ 2+
C*1601 1 1 1+ 1 1 1 1+ 1 1 1+ 1 1 1 1 1+ 3 2 2 2 2 2 2
C*1701 1 1 1+ 1 1+ 1 1 1 2 1 1 1 1+ 2 3+ 2+ 2+ 2+ 2+ 2+ 2+
C*1802 8+ 8+ 9+ 8+ 10 9 9 11+ 11+ 9+ 9+ 9+ 9+ 11+ 15 10+ 10 11+ 14+ 15 15+ 13 12 12 11 10+ 15+ 16 15+
HLA-B Reactivity B*0801 1+ 1
B*1301 1 1 1 1 1 1 1 1 1 1 1+ 2 1+ 1+ 3 1+ 1+ 1+ 2+ 3+ 2+ 3+ 2 2+ 2+ 2+ 3+ 3+ 4
B*1302 1 1 1+ 1 1 1 1+ 1 1 1+ 1+
B*1401 3+ 4+ 4 3+ 5 3+ 4 4+ 5 5+ 5+ 5+ 6 6 8+ 6 5 6+ 8+ 9 9 8+ 8 8 7 7 10+ 10+ 11
B*1402 1 1 1 1 1 1+ 1 1 1 1+ 2 2+ 2 2+ 2 2+ 2+ 2+
B*1502 1 1+ 1 1 1+ 1+ 1+ 1+ 1+ 2 2 2 2 2 4+ 2+ 2 2+ 3 5 3+ 4 3 3 3 3 4+ 4+ 5
B*1503 1 1 1 1 1 1+ 1+ 1+ 1+ 1+ 2 2 2
B*1510 1 1 1 1 1 1 1 1
B*1511 1+ 1+ 1+ 1+ 1+ 1+ 1+ 1+ 1+ 2 2 2+ 2 2+ 5 2+ 2 2+ 3+ 5+ 3+ 4 3+ 3+ 3+ 3+ 4+ 5 5
B*1512 1 1 1 1 1 1 1
B*1513 1 1 1 1 1 1 1 1 1 1+ 1+ 2 1+ 1+ 4 2 1 1+ 2 4+ 2+ 2+ 2+ 2+ 2+ 2+ 3+ 3+ 3+
B*1516 1 1 1 1 1 1 1+ 1+ 1 3 1+ 1 1+ 2 3+ 2 2+ 2 2 2+ 2 3 3 3
B*1801 1+ 1+ 1+ 1+ 1+ 2 2 2 2 2+ 2+ 3 3 2+ 3+ 3 2 3 4 4 4 4+ 4 3+ 4 3+ 5 5+ 5+
B*2705 1 1 1 1 1 1 1 1
B*2708 1 1 1 1 1 1 1+ 1 1 1 1+ 2 2 1+ 2 2 2 2 2+
B*3501 4 4 4+ 4 4+ 4 4+ 5+ 5+ 3+ 4+ 4 5 5+ 7+ 4+ 5 6 8 8 8+ 7+ 5+ 5+ 5+ 5 9 9 9+
B*3701 1 1 2 2 2+ 2 1+ 0 2+ 1 2 2+ 3 2+ 3+ 3 4 3+ 4 4 4+
B*3801 1 1 1 1
B*3901 1+ 1+ 2 1+ 2 1+ 2 2 2 2 2+ 2+ 2+ 2+ 3+ 2+ 2 3 4 4 4+ 4+ 3+ 3+ 3+ 3+ 5+ 4+ 6
B*4001 1 1 ‘ 1 1 1 1 1 1 1+ 1+ 2 2 1+ 2 2 1+ 2 2+ 2+ 2+ 3 2+ 3 3 3 3+ 3+ 4
B*4002 1+ 1+ 2 2 2 2 2 2 2+ 2 2+ 2 2+ 2+ 3+ 2+ 2 3 4 4 4 4 3 3+ 3+ 3 5 5 5+
B*4006 8 8 9+ 8+ 9 8+ 9 11+ 11+ 8 9 8 9+ 11 14 9 9 11 14 14 14+ 12+ 10 10+ 9+ 9+ 14+ 14+ 15+
B*4101 1+ 1+ 2 1+ 2 2 2 2 2 2+ 2+ 2+ 2+ 2+ 3 3 2 2+ 4 3+ 4 4+ 3+ 3+ 3+ 3+ 5 5 5+
B*4402 1 1 1 1 1 1 1 1+ 1 1+ 1 1+ 2 2 2 2+ 2 2+ 2+ 2+
B*4403 2+ 2+ 2+ 2 2+ 2+ 2+ 2+ 2+ 2 3 2+ 3 3+ 4 3+ 2+ 3+ 4+ 4 5 5 4 4 4 4 6 6 6
B*4501 2+ 3 3 2+ 3 3 3 3+ 3+ 3 4 3+ 4 4+ 5 4 3+ 4+ 6+ 5+ 6+ 6 5 5 5 5 7+ 7+ 8
B*4601 1 1 1 1 1 1 1 1 1 1+ 1+ 2 1+ 1+ 3+ 2 1 1+ 2+ 3+ 2+ 3 2+ 2+ 2+ 2+ 3 3+ 3+
B*4701 1 1 1 1 1 1 1 1+ 1+ 2 1+ 1+ 1+ 2+ 1 1+ 2 2 2+ 3 3 3 3 3 3+ 3+ 3+
B*4801 1 1 1+ 1 1 1+ 1 1 1 1+ 2 2 2 2 2 2 2
B*5101 1 1 1 1 1 1+ 1+ 1 1+ 1+ 1+ 2 2 2 2 2 2+ 2+ 2+
B*5102 1 1 1 1 1 1 1 1+ 1+ 1 1+ 2 2 2 2+ 2 2 2 2 3 3 3+
B*5201 1 1 1+ 1 1 1 1+ 1 1+ 1+ 1+ 2 2+ 2+ 2+ 2 2+ 2+ 2+
B*5301 2 2+ 3 3 3 2 3 4 4 3 3+ 3+ 4 4+ 5+ 3+ 3+ 4+ 6+ 6 6+ 6+ 4+ 4+ 5 4+ 7+ 7+ 8
B*5401 1+ 1+ 1+ 1+ 1+ 1+ 1+ 1+ 1+ 1+ 2 2 2 2 2+ 2 2 2+ 3+ 3 3+ 4 3 3 3 3 4+ 4+ 5
B*5501 1 1 1 1 1 1 1 1 1+ 1+ 1+ 2 1+ 2 2 2+
B*5801 3+ 3+ 4 3+ 4 4 4 5 5 4 3+ 4+ 5+ 7 4+ 4+ 5+ 7 7+ 7+ 7+ 4+ 5 5 5 8+ 9 9+
B*5901 1 1 1 1 1+ 1 1 1 1 1+ 2 2 2 2 2 2 2+
B*7301 3 1 1 1 1 1 1 1 1+ 1+ 1+ 1+ 1+ 2 2 2
B*7801 1+ 1+ 1+ 1+ 1+ 1+ 1+ 2 2 1 2 2 2 2+ 3 2 2 2+ 3+ 3 3+ 4 3 3 3 3 4+ 5 4
B*8101 1+ 1
B*8201 1 1 1 1 1 1 1 1 1 1+ 1+ 1+ 1+ 1+ 2 2 1 1+ 2+ 2 2+ 3 3 2+ 3 2+ 3+ 3+ 3+
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Note: For HLA-E only HLA-EE107 was tested for MFI.

Despite such high recognition of HLA-Ia alleles, it is interesting to note the failure to recognize HLA-Ib alleles other than that of HLA-E. Only one allele from each HLA-F and HLA-G was tested, and probably more alleles need to be tested to confirm their lack of affinity of HLA-F and HLA-G. Subgroups A, B, C, D. E, and F in Table 6 also illustrate the two important aspects of the mAbs, namely (1) progressive increase in the number of alleles recognized by the mAbs from subgroup A to E, and similarly, (2) the progressive increase of MFI of these mAbs for each of the alleles also from subgroup A to E. Indeed, all the 29 mAbs recognized B*4006 positively, with a progressive increase in MFI from subgroup A to F.

3.5. Expanding Diversity HLA-Ia Reactivity of HLA-EG mAbs

3.5.1. HLA-B Reactive but HLA-A, HLA-C, HLA-F, and HLA-G Non-Reactive (Group G-V)

Four mAbs (TFL-173/174/175/219) represented by Group G-V in Table 7 illustrate a unique feature of allelic specificity not observed earlier among HLA-ER mAbs, that is they reacted only with a few B alleles in addition to HLA-E. Strikingly, TFL-175 recognized B*1511 and B*5201, while the other three mAbs recognized B*5701 in addition. It is important to note that a distinguishing feature of anti-HLA-EG mAbs is that they do not recognize HLA-B*4006, in striking contrast to anti-HLA-ER. Figure 5 shows several shared amino acid sequences between HLA-E and B*1511. Since HLA-E mAbs recognize non-linear sequences, it is possible that the anti-HLA-E mAbs recognize unique conformation of the shared amino acid sequences of B*1511, B*5201 and B*5701.

Table 7.

HLA-EG mAbs: HLA-F, HLA-G, and HLA-A non-reactive (G-II) or reactive only to HLA-A*1101, and to both HLA-B and HLA-C alleles.

MFI Expressed in Thousands
Groups Subgroups number of mAbs mAb Nomenclature IgG Subclass HLA-ER Reactivity (MFI) HLA-EG Reactivity (MFI) HLA-F Reactivity (MFI) HLA-G Reactivity (MFI) HLA-A*1101 Reactivity (MFI) HLA-B Reactivity HLA-C Reactivity
B*0702 B*0801 B*1301 B*1302 B*1402 B*1501 B*1510 B*1511 B*1512 B*1513 B*1516 B*2708 B*3501 B*3901 B*4001 B*4002 B*4201 B*4402 B*4501 B*5001 B*5201 B*5301 B*5401 B*5601 B*5701 B*5703 B*5801 B*8101 C*0401 C*0702 C*1402 C*1701 C*1802
G-V 1 TFL-175 IgG1 12 1+ 1+
2 TFL-174 IgG1 12 1+ 1+ 1
3 TFL-173 IgG1 12 1+ 1+ 1
4 TFL-219 IgG1 21 20+ 2 2 1
G-VI [A] 1 TFL-222 IgG1 19 18+ 1 3+ 2 1+ 1
2 TFL-220 IgG1 19 19+ 1 3+ 2 1+ 1
3 TFL-187 IgG2a 25 24+ 1+ 3+ 2 1+ 1
[B] 4 TFL-201 IgG1 11 12 1+ 1 3 1+ 2+ 1+ 3
5 TFL-193 IgG1 14 15+ 2 1 1 5 2+ 3+ 2+ 1 4 1
6 TFL-194 IgG1 21 21 2 1 1+ 5+ 3 3+ 3 1 4+ 1
7 TFL-199 IgG1 15+ 15+ 2 1 1+ 4+ 2+ 3+ 2+ 1 4 1
8 TFL-258 IgG1 20+ 20+ 3+ 0 1 7 1 1 4+ 4 3 1
9 TFL-257 IgG1 20+ 21+ 3+ 1 1 7 1 1 4 4 3 5 1
[C] 10 TFL-253 IgG2b 26+ 26 6 1 2+ 2+ 1 11 1 1 1 2 1+ 1 2 7+ 1 1 7+ 1+ 1 7 5+ 1+ 8+ 2
11 TFL-249 IgG2b 26+ 25 6 1 2+ 2+ 1 11 1 1 1 2 2 1+ 1 2 7+ 1 1 1 1 7+ 1+ 1 7 5+ 1+ 9 2
12 TFL-250 IgG2b 27 26 6 1 2+ 2+ 1 10+ 1 2 1+ 1 2 7+ 1 1 1 7+ 1 1 7 5+ 1+ 8+ 2
13 TFL-241 IgG1 22 21 4 2+ 13+ 2 3 8+ 1+ 7+ 14 12 1+ 1 1 14 1 5 5+ 11 5 4+ 4 1 6 3+
14 TFL-242 IgG1 22 21+ 4+ 2+ 14 2 3+ 9 1+ 7+ 14 12+ 1 1+ 1 1+ 14+ 1 5 1 5+ 11 5 5 4 1 6 4
15 TFL-247 IgG1 21+ 20+ 4 2+ 14 2 3 8+ 1+ 7+ 14+ 12+ 1+ 1 14+ 1 5 1 5+ 11+ 5 1 4+ 1 6 4
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3.5.2. HLA-Ia Reactive but HLA-F, and HLA-G Non-Reactive (Group G-VI)

Group G-VI in Table 7 further illustrates expansion of allelic recognition of HLA-EG mAbs, which included recognition of A*1101 only among alleles of HLA-A isoforms, very similar to Group R-IV of HLA-ER mAbs. It is important to note a progressive increase in the MFIs of Group VI subgroups concomitant with the increase in MFIs of alleles of B and C loci. Such increase in MFI does not affect the MFIs of HLA-E, suggesting the expansion of allelic recognition together with the increase in the mAbs reactivity. No striking increase in MFI was observed for the mAbs that reached saturation for HLA-E recognition, and there was a need to dilute these mAbs further to assess the increase in the mAb strength at the specified concentration. Serial dilution of the mAbs reveal relative affinity of the mAb for different alleles. Group VII in Table 8 illustrates the same, the mAbs in the subgroups are HLA-Ia reactive but HLA-F and HLA-G are non-reactive. Eleven anti-HLA-EG mAbs in Groups B to D recognized B*4006 at low MFI (<2 K).

Table 8.

Evolving HLA-EG mAbs: HLA-F and HLA-G non-reactive but reactive to HLA-A, HLA-B, and HLA-C alleles.

Groups G-VII
MFI Expressed in Thousands
Subgroups [A] [B] [C] [D] [E] [F] [G]
Number of mAbs 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25
mAb Nomenclature TFL-221 TFL-218 TFL-217 TFL-216 TFL-196 TFL-167 TFL-231 TFL-252 TFL-170 TFL-169 TFL-166 TFL-168 TFL-251 TFL-207 TFL-206 TFL-205 TFL-243 TFL-246 TFL-244 TFL-245 TFL-189 TFL-190 TFL-188 TFL-172 TFL-171
IgG Subclass NT IgG1 IgG1 IgG1 IgG2a IgG1 IgG2b IgG2b IgG1 IgG1 IgG1 IgG1 IgG2b IgG1 IgG1 IgG1 IgG2a IgG2a IgG2a IgG2a IgG1 IgG1 IgG1 IgG1 IgG1
HLA-ER Reactivity 25 21 20 20 24 24+ 25 26 18+ 19 26 24+ 25 25 13+ 14+ 14
HLA-EG Reactivity 25 20 19+ 20 24 15 24 25 17 17+ 16+ 15 25+ 17+ 19 18+ 25 24+ 24+ 24+ 15+ 16+ 16 14+ 15
HLA-F Reactivity
HLA-G Reactivity
HLA-A Reactivity A*0101 14 15
A*0201 1 1
A*0203 1+ 1+
A*0206 1 1 1 1 1 1 1 1 1+ 1+ 1+
A*0301 12+ 14
A*1101 6 5 5+ 5+ 4 5+ 8+ 9+ 6+ 6+ 6+ 6+ 9+ 8 8 8 12 12 12 12 1+ 1+ 1+ 9+ 10
A*1102 11 10+ 11+ 11 11 11+
A*2402 1 1 1 1 1 1+ 2+ 3 2 2 2 2 3 3 3 3 2+ 2+ 3 3 1 1
A*2403 1 1 1 1 1 1 1 1+ 1+ 1+
A*2601 1 1+ 2 1+ 2 2 2 2 2+ 3 3 3 1 1
A*2901 1 1 1+ 1+ 1+ 1+ 1+ 1+ 2 2 2
A*3001 1 1 1 1 1 1 1+ 1+ 1+
A*3002 1 1 1 1 1 1 1 1+ 2 2 2
A*3201 1 1 1 1 1 1 1 1+ 1+ 1+ 1+
A*3301 1 1 1 1 1 1 1 1+ 1+ 1+
A*3303 1 1 1+ 1+ 1+ 1+ 1+ 1+ 2 2 2 14+ 15
A*3401 1 1 1 1 11 12
A*3402 1 1 1 11+ 12
A*3601 1 1 1 1 1 1+ 1+ 1+ 11 12
A*4301 1 1 1 11+ 12+
A*7401 1+ 1+ 1 1 1 1+ 1+ 1+ 1+ 1+ 2 2+ 13+ 14
A*8001 3 3+
HLA-B Reactivity B*0702 1 1 1
B*0801 15+ 3 3 3 1+ 2+ 23 4 3 3 3 3 4 20+ 19 20 1+ 1+ 1+ 1+ 11 11+
B*1301 16+ 17 17+ 17+ 2 4 24 6 4+ 4+ 4+ 4+ 6 20+ 19+ 20 3 3 3 3 <1 1 1 2 2+
B*1302 3 3 3 3 2 3+ 6 6+ 4+ 4+ 4+ 4+ 7 6 6 6 8 9 8 8+ <1 <1 1 2 2+
B*1401 1 1+ 1 1 1 1 1+ 2 2 2 7+ 7 7+ 8
B*1402 4 7+ 8 8+ 11 12+ 12+ 12+
B*1501 10+ 12+ 12+ 13 1 2 17+ 3+ 2+ 2+ 2+ 3 3+ 16+ 16+ 16+ 6 6 6 6+ 1 1
B*1502 1 1 1 1 1 1 1+ 1+ 1+ 15+ 15 15+ 15+ 12 13
B*1503 1 1 1 1+ 1+ 1+ 10 9+ 10 10+
B*1510 1+ 4 4+ 5 5+ 8 7+ 8 1+ 1+
B*1511 10+ 8+ 9 8+ 6+ 8 13+ 14 9 9 9 9 14 11+ 10+ 11 10+ 10+ 10 10 4 4+ 4+ 11+ 12
B*1512 17+ 17+ 17+ 18 1 2+ 23+ 3+ 3 3 3 3 3+ 20 20 19+ 8+ 7+ 8+ 9 1 1
B*1513 12+ 15+ 16+ 17 1 22 1 1 1 1 1 1 20 18+ 19 7+ 7 8 8+ 14+ 15+
B*1516 1+ 1+ 1+ 1+ 1 2 3 3+ 2+ 2+ 2+ 2+ 3+ 3+ 3+ 3+ 1 1+ 1+ 1+ 1 1
B*1801 1 1
B*2705 1 1 1 1
B*2708 1 1 1+ 1+ 1 2 2+ 3+ 2+ 2+ 2+ 2+ 3+ 3+ 3 3+ 1 1 1 1 1
B*3501 1 1+ 1+ 1+ 1 2 3+ 4 3 2+ 3 3 4 4 4 4 1+ 1 1+ 1+ 13 14+
B*3701 0 0 0 0 1 1 1
B*3901 2+ 2 2+ 2+ 1+ 3 4+ 5+ 3+ 3+ 3+ 3+ 5+ 5 5 5 2+ 2+ 2+ 2+ 11 12+
B*4001 1 1 1 1 1+ 2 2+ 2 2 2 2 2+ 3 3 3 1 1 1 1 7 7+
B*4002 2 1+ 2 2 1+ 2+ 4 5+ 3+ 3 3+ 3+ 5 4+ 4+ 4+ 2+ 2+ 2+ 2+ 9 9+
B*4006 1 1 1+ 1+ 1+ 1+ 1+ 1+ 2 2 2
B*4201 1+ 1 1+ 1+ 1 2 3 4 2+ 2+ 2+ 2+ 4 4 4 4 3 2+ 3 3 12+ 13+
B*4402 2+ 2 2 2 1+ 3 4+ 5+ 3+ 3+ 3+ 3+ 5+ 5 5 4+ 2+ 2+ 2+ 2+ 8+ 9
B*4403 0 0 0 0 1 1 1 1 1 1 1+ 1+ 1+ 6 6
B*4501 16+ 18 18+ 18+ 1+ 23+ 2 2 1+ 2 2 2 20 20 20+ 13+ 15
B*4601 1 1 1+ 1 1 1 1 1+ 2 2 2 10+ 11+
B*4701 1 1 1 1 1 1 1 1+ 1+ 1+ 12 14
B*4801 3+ 2+ 3+ 4 9 9+
B*4901 4+ 4 5 5 8+ 9+
B*5001 1 1+ 1+ 1+ 1 2 3+ 4 2+ 2+ 2+ 2+ 4 4 4 4 6+ 5+ 6+ 7 1 1
B*5101 1 1 1+ 1 1 1 1 1+ 2 2 2 5 4 5 5+
B*5102 1 1 1 1 1 1 1+ 1+ 1+ 2 3+ 3 4 4 11 12
B*5201 7 6 6+ 6+ 4+ 6+ 10+ 11 7+ 7+ 7 7+ 11 9+ 9+ 9+ 6+ 7 6+ 7 2 2+ 2+ 4+ 5
B*5301 1 1 1+ 1+ 1 2 2 3+ 2+ 2+ 2+ 2+ 3+ 3 3 3 1 1 1 1+ 1 1
B*5401 1 1 1 1 1 1+ 2+ 3+ 2 2 2 2 3+ 3 3 3+ 15+ 15+ 15 15 1 1
B*5501 9+ 1 1 1+ 1 1 1 1 1+ 1+ 1+ 1+ 0 0 0 0
B*5601 7 9 9+ 15 1 1+ 12+ 2+ 2+ 2 2+ 2+ 3 13 12+ 12+ <1 1 1 1 2 2
B*5701 13 14+ 14+ 6+ 1 2+ 20 4 3 3 3 3 4 17+ 17+ 18 1 1 1 1 4+ 5
B*5703 7+ 6+ 6+ 2 4+ 6 10+ 10+ 7 7 7 7 10+ 9 8 9 7 7 6+ 7 2+ 3 2+ 7 7+
B*5801 1+ 1+ 2 1 1 2+ 4 4+ 3 3 3 3 4+ 4+ 4+ 1+ 1+ 1+ 1+ 2+ 3 3 1 1+
B*6701 1 1 1 1+ 1 1+ 2 2+ 2 2 2 2 2+ 3 3 3 1
B*7301 1 1+ 2+ 1+ 1+ 1+ 1+ 2+ 2+ 2+ 2+ 12+ 12 12 13
B*7801 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0
B*8101 1 1 1+ 0 1 2 3 4 2+ 2+ 2+ 2+ 4 3+ 3+ 3+ 1+ 1+ 1+ 1+ 1 1
B*8201 1 1 2 1+ 1+ 1+ 1+ 2+ 2 2 2 12 13 13 14 16
HLA-C Reactivity C*0102 1 1 1 1 1 1 1+ 1+ 1+ 1+ 10+ 11+ 11+ 14 15+
C*0202 1+ 1 2+ 1+ 1+ 1+ 1+ 2+ 2+ 2+ 2+ 13+ 11+ 13+ 13+ 13+ 13+ 14+ 13+ 15
C*0302 1 1 1 1 1 1 1+ 1+ 1+ 8 6+ 8+ 8 16+ 16+ 16+ 14 15+
C*0303 1 1 1 6 1 2 2 3+ 2+ 2+ 2+ 2+ 3+ 3+ 3 3+ 9 7+ 9 9 15 15 15 14 15+
C*0304 1 1 2 1+ 1+ 1+ 1+ 2 2 2 2 5+ 6+ 6+ 13+ 15
C*0401 6+ 5+ 6 5+ 4+ 6 9 10+ 7 7 6+ 7 10+ 8+ 8+ 8 6+ 6+ 6+ 6+ 10 11+ 11 13 14
C*0501 1 1 2 1+ 1+ 1+ 1+ 2 2 1+ 2 1 1 1 1 20 20 19 13 14+
C*0602 1 1 1+ 1 1 1 1 1+ 1+ 1+ 1+ 11+ 10 12 12 17+ 17+ 18 12 14
C*0702 5+ 5+ 5+ 1 9+ 6+ 6+ 6+ 6+ 9+ 8 7+ 7+ 5+ 5+ 5+ 5+ 11 12+ 12+ 13+ 15
C*0801 1 1 1 1 1 1 1+ 1 1 20 20+ 20 15+ 17
C*1203 1 1 2 1 1+ 1+ 3 2 2 2 2 3 2+ 2+ 2+ 1 1 1 1 13 13+ 14 13+ 14+
C*1402 1+ 1+ 2 7+ 1+ 2+ 3+ 4+ 3 3 3 3 4+ 4 3+ 4 19+ 19+ 19+ 19+ 18+ 18+ 18 14+ 16
C*1502 1 1 2 1 1 1 1 2 2 1+ 2 24+ 24+ 24+ 24+ 18+ 19 18+ 13+ 15
C*1601 1 1 11 2 1+ 1+ 1+ 1+ 2+ 2+ 2+ 2+ 22 21+ 21+ 21+ 16 16+ 17 13 14+
C*1701 8+ 7 7+ 4+ 5 7 24+ 12 8 8 8 8 12 10 9+ 9+ 8 8 8 8 11+ 12+ 12+ 14+ 16
C*1802 18 4+ 4+ 0 1+ 3+ 5+ 4 4 4 4+ 6 20+ 20 20+ 2+ 2+ 2+ 2+ 1 1 1 13 14+
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3.6. Emergence of Highly Polyreactive mAbs against HLA-E*01:01 and HLA-E*01:03

HLA-Ib Specific mAbs Also Eact with all HLA-Ia Isoform Alleles

HLA-ER as an immunogen has elicited eight HLA-Ib and HLA-Ia reactive antibodies (Table 9). Of these, TFL-069 (R-VII) showed HLA-F reactivity and HLA-A*1101 reactivity in addition to HLA-B and HLA-C reactivities. The MFIs of B*4006 and C*1802 are 7 K and 8 K, respectively. Two mAbs, namely TFL-103 and TFL-104 (R-VIII), failed to react with HLA-F, but reacted well with HLA-G (MFI: 4 K) and with several HLA-A, HLA-B, and HLA-C alleles. Three mAbs showed unique affinity to both HLA-F and HLA-G in addition to HLA-I alleles. Of these mAbs, TFL-049 recognized only HLA-A*1101, four B alleles including B*4006, and three C alleles, including C*1802 (Table 9). The other two mAbs are polyreactive, of which TFL-006 supersedes all anti-HLA mAbs studied so far as being the most highly polyreactive; they recognized 25 A alleles, 45 B alleles, and all the 16 C alleles. Evidently, it recognizes the most common sequences in HLA isoforms located at the α2 helical domain, namely 117AYDGKDY123 and 126LNEDLRSWTA135. Indeed, the synthetic peptide AYDGKDY inhibited the binding of TFL-006 and TFL-007 to HLA-I coated beadsets [36]. Figure 4 illustrates the location of the possible epitopes recognized by TFL-006 and TFL-007.

Table 9.

Evolving HLA-ER and HLA-EG mAbs: HLA-Ib mAbs also react to all HLA-Ia isoform alleles.

Groups R-VII R-VIII R-IX R-X G-VIII G-IX G-X
MFI Expressed in Thousands
Subgroups [A] [B] [C] [A] [B]
Number of mAbs 1 1 2 1 1 2 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 1 2 3 4
mAb Nomenclature TFL-063 TFL-103 TFL-104 TFL-049 TFL-006 TFL-007 TFL-227 TFL-197 TFL-198 TFL-202 TFL-203 TFL-204 TFL-210 TFL-211 TFL-212 TFL-235 TFL-230 TFL-256 TFL-224 TFL-214 TFL-213 TFL-215 TFL-225 TFL-233 TFL-240 TFL-238 TFL-236 TFL-237 TFL-239 TFL-234 TFL-248 TFL-229 TFL-232 TFL-177 TFL-176 TFL-198
IgG Subclass IgG2b IgG1 IgG1 IgG2b IgG2a IgG2a IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG3 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG2b IgG3 IgG1 IgG1 IgG1
HLA-ER Reactivity 22+ 18 17+ 15 22+ 21+ 18 17 16 18 18 17+ 19 18 18 21 0 22+ 22 20+ 21 21 19 19+ 18 18 19 20 19+ 19 21+ 30 22+ 14+ 13+ 15
HLA-EG Reactivity 18+ 16 15 17+ 16+ 16+ 17+ 17+ 18 19+ 18+ 22 20+ 21+ 20 20+ 19+ 18 19+ 19+ 18 21 27+ 21
HLA-F Reactivity 3+ 8+ 12+ 11 5+ 10+ 10 11 10+ 10+ 11 11 11 9 2+ 5 4+ 10
HLA-G Reactivity 4+ 4+ 7+ 7+ 2+ 1 1 1 2 2+ 2+ 1 1+ 11+ 14 13+ 9+ 8 10 2 18+ 21+ 11 11 0+
HLA-A Reactivity A*0101 2 1 5+ 13 1 1 1 4 0 0 0
A*0201 3
A*0203 1 2+ 2 2+ 2+ 2+ 2 2 2 4+ 2
A*0206 1 3+ 2+ 3+ 3+ 3+ 3 3 3 4+ 0 0 2+
A*0301 3 7 5+ 8+ 5 5 3+ 3 3+ 10 5+
A*1101 2 6 6 1 10 8 4 11 10 11+ 11+ 11 11+ 11 11 11 4+ 4+ 4+ 5 5 5+ 5+ 5+ 5+ 5 5+ 7 6+ 7 7+ 9+ 3+ 4 10
A*1102 3 1 1 1+ 1 1 1 1 1 9 1
A*2301 3+ 9+ 8 11 10 10+ 10 10 10 11 8
A*2402 2+ 3 3 2 4+ 14+ 13 15+ 14+ 14+ 14 14 14+ 12+ 1 1+ 1 1 1+ 1+ 1 1 2+ 2+ 3 1+ 2+ 1+ 1+ 13
A*2403 2+ 2+ 3 1+ 4+ 12+ 11 13+ 13+ 13 13+ 12+ 13 12 1 1 1+ 1 1 1 11
A*2501 1 4
A*2601 3 1+ 1 1 1+ 1 1 1 1 1 8 2 2+ 2+ 2+ 2 2+ 1+ 2 2 2 1
A*2901 1+ 1+ 3 2 5 4+ 6+ 5 5 4+ 4 4+ 6+ 1 1 1 1 1 1 1+ 4+
A*2902 2 1 6+ 5 7+ 6 5+ 4+ 4 4+ 6 0 0 5
A*3001 2 1 5 5 4 6+ 4+ 4+ 4 3+ 4 12 1+ 1 1+ 1 1 4
A*3002 1 1 3 2 3 7 6 8 6+ 6+ 6+ 6 6+ 9+ 1 1 1 1 6
A*3101 4 3 5 3 3+ 2+ 2 2 3 3
A*3201 1 1 2 1+ 3+ 2+ 4+ 3 3 2+ 2+ 2+ 5 1 1 1 1 1 2+
A*3301 1 1 2+ 1+ 6+ 5+ 7+ 6+ 6+ 5+ 5 5+ 5+ 1 1 1 1 1 1 5+
A*3303 2 2 4 2+ 1+ 7 6+ 8 7 7+ 7+ 7+ 7+ 8+ 1 1 1 1+ 1 1 1 1+ 6+
A*3401 1+ 1+ 6 3+ 1+ 1 1+ 1+ 1+ 1+ 1+ 1+ 3+ 1
A*3402 1 4 4+ 4 5 4+ 5 5 4+ 5 11+ 1 0 1 4
A*3601 2 2 5+ 3+ 1 1 1 1 1 1 1 1 8+ 1 1 1 1 1 1 1 1
A*4301 1 4 2 5
A*6601 3+ 1+ 6+ 5+ 7+ 6+ 6+ 5+ 5 5 4+ 5+
A*6602 1 3+ 3 4+ 3+ 3+ 3 3 3 2+ 3
A*6801 1 5+ 4+ 6+ 5+ 5+ 5 4 4+ 5 4+
A*6802 1 1 2 1 2 1+ 2 2 2 1+ 1+ 1+ 8 1+ 2 1+
A*6901 1+ 6+ 5 7+ 5+ 5 3+ 3+ 4 4+ 5
A*7401 1 2+ 2 2+ 2+ 2+ 2+ 2 2+ 10 1 1 1+ 1 1 1 2
A*8001 2+ 1 2 13 13 13+ 13 13 13 12+ 12+ 9 13
HLA-B Reactivity B*0702 1 4+ 18 17+ 18+ 18 18 17+ 17 18 13+ 2 1+ 2 17+
B*0801 2 1 7 2+ 2 3 2+ 2+ 2+ 2+ 2+ 15 1 1 1 1 1 1+ 1+ 1+ 2 2 2 19+ 19+ 19+ 2+ 24+ 1+ 2 2 2
B*1301 2 2+ 5+ 3+ 8+ 18+ 19 19_ 19 18+ 18+ 18 18+ 16 2 2 2 2+ 2+ 2+ 2+ 2+ 3+ 3 3 19 20+ 19+ 4+ 24 3+ 3+ 4 18
B*1302 1 2 1 6 13+ 12+ 14+ 13+ 14 13+ 13+ 13+ 13+ 2 2 2 2+ 2+ 2+ 2+ 3 3 3 3+ 6 6 6 3+ 6+ 2+ 2+ 12+
B*1401 2 7 7 2 11 8+ 6 15+ 14+ 16 16 15+ 16 16 15+ 14 3 2+ 3 1+ 1 14+
B*1402 1+ 1+ 4 2+ 2 9+ 8 10 10 10+ 9+ 9 9+ 8+ 12+ 12+ 12+ 11+ 8
B*1501 1 4 5+ 4+ 6 5+ 6 5+ 5+ 5+ 11+ 1 1 1 1 1 1 1 1 1 1 16 16 16 2 18+ 1+ 2 4+
B*1502 3 3 6 4 6+ 19 18+ 19+ 19+ 19 19 18 18+ 15 1 1+ 1+ 2+ 2 2+ 5 8 1 1 18+
B*1503 1 1 2+ 1+ 6+ 16+ 16+ 17+ 17+ 17 17 16+ 18+ 14 1+ 1+ 1+ 1 1 1 16+
B*1510 2+ 1 1 8 7 8+ 8 8 7+ 7+ 7+ 6+ 8 7+ 8 5 7
B*1511 3 3+ 9 5+ 9 15 14+ 16 15+ 15+ 15+ 15 15+ 16+ 7+ 7+ 8 8+ 8+ 9 9 9 9 9 9+ 10+ 10 10+ 10+ 16 1 6 7 14+
B*1512 1+ 7 3+ 3 4+ 4 4 3+ 3 3+ 15 1 1 1 1 1 1 1 1 1+ 1+ 1+ 9 19 19+ 2 24 2+ 2 2+ 3
B*1513 2 2 5 3 6+ 1+ 1 1+ 1+ 1+ 1+ 1+ 1+ 15 19 19+ 18+ 22+ 4 1 1 1
B*1516 2 2 5+ 3 4+ 15 13+ 15+ 15+ 15 15 14+ 14+ 12 1 1 1 1 1 1 1 1 1 1 2 3 3 3 2+ 3 1+ 2 13+
B*1801 3+ 3+ 6+ 4+ 2 10+ 10 11+ 11 10+ 10+ 10+ 10+ 7+ 10
B*2705 2+ 1+ 1 8+ 7+ 8+ 8+ 8+ 8 7+ 8 6+ 7+
B*2708 1 1 4 2+ 4+ 5+ 4+ 6 5+ 5+ 5+ 5 5+ 11+ 1 1 1 1 1 1 1 1 1 2+ 2 2+ 2 2+ 2 2 4+
B*3501 2 6+ 6+ 1 10 8+ 7+ 19 19 20 19+ 18+ 18 18 18 16 1 1 1+ 1 1 1 3 3+ 3+ 8+ 8 9 2+ 8 9 2 2 19
B*3701 2+ 3 6 4 6 15+ 15 16 16 16+ 15+ 15+ 15+ 14 1 1 1 1 1 15
B*3801 3+ 1+ 3+ 13+ 12+ 14 13+ 13+ 13 12+ 13+ 11 2 2 3 3 3 4 4 4 4 5 12+
B*3901 3+ 3+ 7 5 7+ 19+ 19 20+ 20 19+ 19+ 18+ 19 15+ 1+ 2 1+ 2 2+ 2 2+ 3 19
B*4001 2+ 2+ 5+ 3+ 4+ 15+ 15 16+ 16 15+ 16 15 15+ 12+ 1 1 1 1 1 2 1+ 2 2 2 1 1 15
B*4002 3+ 3+ 6 4+ 6 4 3+ 4+ 4 4+ 4 4 4 14 1+ 1+ 1+ 1+ 1+ 2 2 1+ 2 2 2+ 3 3 3+ 3+ 4 2 2+ 3+
B*4006 7 11 11 3 15+ 13+ 4+ 3+ 5+ 4+ 4+ 4 3+ 4 3+ 1 1 1 1 1 1 3+
B*4101 3+ 3+ 7 5 3 15+ 15 15+ 15+ 15+ 15 15 15 10+ 15
B*4201 3 9+ 8+ 11 10 10 9+ 9 9 14+ 1 1 1 1 1+ 1+ 1 1 2+ 2+ 3 3+ 3+ 3+ 2+ 9+ 12+ 2+ 3 8+
B*4402 1+ 1+ 7 4 1+ 6+ 6 7+ 7 7 6 6 6 10 1+ 1+ 1+ 2 2 2 2 2 2 2 2 3+ 3+ 3+ 3+ 4+ 2 2+ 6
B*4403 4 4 7 5+ 3+ 1 1 11 1
B*4501 1 5 5+ 9+ 7+ 6+ 19 19 20+ 19+ 19 19 19 19 16 1 19+ 21 20 1+ 25 2 2 2 19
B*4601 2+ 2+ 6 4 6+ 17+ 17 19 17+ 18 17+ 17 18 14+ 1 1 1+ 1 2 1 1 1 17
B*4701 2 2 6+ 3+ 8+ 20 20 21 19+ 19+ 19 19 19 16 1+ 2 2+ 3+ 3 3+ 2 2 1+ 1+ 20
B*4801 1 1 4 2+ 7 14+
B*4901 5+ 13+
B*5001 8 4 3+ 4+ 4+ 4+ 4+ 4 4+ 15+ 1 1 1 1 1 1+ 1 1 1+ 1+ 1+ 5 4+ 5 2+ 3+ 2+ 2+ 3+
B*5101 1+ 1+ 6 3+ 7+ 1+ 1+ 2 1+ 1+ 1_ 1+ 1+ 15 2+ 2 2+ 1 1 1+ 1+ 1+
B*5102 2 2 5 3+ 7 2 1+ 2 2 2 2 2 2 15+ 3 2+ 3+ 1 1 1 1+
B*5201 1+ 1+ 4+ 2+ 9+ 10 9 11 10+ 10 10 9+ 10 15+ 5+ 5+ 5+ 6 6 6+ 6+ 6+ 6 6+ 6+ 8+ 9 9 8 12 5+ 6+ 9
B*5301 1 5+ 5+ 8+ 7 6+ 2+ 2+ 3 2+ 2+ 2+ 2+ 2+ 14 1 1 1 1 1 1 1 1 1+ 1+ 1+ 1+ 1+ 1+ 2 2+ 1+ 2 2+
B*5401 3 3+ 5+ 4 5 2+ 2+ 3 3 3 2+ 2+ 2+ 12+ 1 1 1 1 1 1 1 1 1+ 1+ 1+ 3+ 3 3+ 1 3 1+ 1+ 2+
B*5501 2+ 1+ 2 2+ 2 2+ 2+ 2+ 2 2 2 8 1 1 1 1 1 2
B*5601 1 2+ 4+ 3+ 4+ 4+ 4+ 4 4 4+ 10 1 1 1 1 1 1 1 12+ 12+ 12+ 2 13+ 1 1 3+
B*5701 5 5+ 4+ 6 5+ 5+ 5+ 5+ 5+ 13 1 1 1 1 1 1+ 1 1 1+ 1+ 1+ 17+ 18 17+ 2+ 21+ 2 2 4+
B*5703 1 7 12+ 11+ 13 12+ 12+ 12+ 12+ 12+ 13+ 5 5+ 5+ 6 6 6+ 6+ 6 6+ 6 6+ 8 8+ 8 8 12+ 1 5 5+ 11+
B*5801 2 6 6+ 1 10 8 9 2+ 2 2+ 2+ 2+ 2+ 2+ 2 17 1 1 1 1+ 1+ 1+ 1+ 1+ 2+ 2+ 2+ 6 5+ 6 2+ 5 1 2+ 3 2
B*5901 1 1 5+ 3 2 9 8 10 9+ 9+ 9 9 9 8+ 8
B*6701 4+ 4+ 4 5 4+ 4+ 4+ 4+ 4+ 11 2 2 2 2 2 1 1+ 4
B*7301 1 1 3 2 6+ 4 3+ 4 4 4 4 3+ 4 14 1 1 1 3 3 3 1+ 2 1+ 1+ 3+
B*7801 3 3+ 6 4+ 5 18+ 17+ 19 18+ 18+ 17+ 17+ 18 13+ 1 1 1 17+
B*8101 1 7+ 19+ 19+ 20+ 19+ 19+ 18+ 19+ 18+ 16 1 1 1 1+ 1+ 1 1 2 2+ 2+ 4+ 4+ 5 2+ 5+ 4 2 2 19+
B*8201 3 2+ 4 3 13 20 19 20+ 20 19+ 19+ 19+ 19+ 18+ 1+ 1+ 1+ 1 1 1 1+ 2+ 3 3 3+ 19
HLA-C Reactivity Cw*0102 7 3 11+ 20 29 20+ 20+ 20 20 19 19+ 18+ 1 1 1 2 2 2 2+ 19
Cw*0202 2+ 2+ 10+ 6 11 15+ 14 16+ 15+ 15+ 11 10+ 11 16 1 1 1 1 1 1 1 1+ 2 2 14
Cw*0302 1+ 1+ 5+ 3 10 15+ 1+ 2
Cw*0303 2 2+ 7 4 9+ 7+ 6+ 8+ 8 7+ 7 6+ 7 15+ 1 1 1 1 1 1+ 1+ 1+ 1+ 1+ 1+ 2 2+ 2+ 3 6+
Cw*0304 2 2 6+ 3+ 11 2 1+ 2+ 2+ 2+ 5+ 5 5 17 1 1+ 1+ 1+ 2+ 3+ 1+ 2 1+
Cw*0401 2+ 1 9+ 18 18 19+ 19+ 19 18 18+ 18 15+ 5 5 5+ 5+ 6 6 6+ 6 6 6 6 6+ 6+ 6+ 7+ 12+ 2 5+ 6 18
Cw*0501 4 8 8+ 2 16 13 13 7 6+ 8 7+ 7+ 12 12 12+ 18+ 2+ 3+ 3+ 1 1 1 1 5+ 13+ 2+ 2+ 6+
Cw*0602 1 1 9 4 16+ 5 4+ 6 5+ 5 10 9+ 10 18+ 1+ 2 2 1 4+ 9+ 6 7 4+
Cw*0702 12 6+ 10 19+ 17+ 19+ 19 19+ 18+ 18+ 19 17 4 5 5 5+ 5+ 5+ 6 6 6+ 6+ 10+ 1 5+ 6 17+
CW*0801 3 6 6+ 1+ 13 10+ 11 21+ 20 22 21 21 21 21 21 19 1 1+ 1+ 2+ 5 1 1+ 20+
Cw*1203 5 2 12 19+ 19+ 21 20 19+ 19+ 19 19 17 1 1 1+ 2 2 1+ 1 1+ 2 3 2+ 2+ 3 19+
Cw*1402 2 2 8+ 4+ 9 10+ 9+ 11 10+ 10 9+ 9+ 9+ 16+ 1+ 1+ 2+ 3 3 2 2 2+ 3 5 2+ 2 2+ 9+
Cw*1502 1+ 1+ 6 3 11 20+ 20+ 22 20+ 20 20 19+ 19+ 18+ 1 1 1 1+ 1+ 1+ 1+ 2 2 1 1+ 4 7+ 2+ 3 20+
Cw*1601 1 1+ 8 4 15+ 19+ 19+ 21 19+ 19 18+ 19 18+ 18 1 1+ 1+ 1+ 1 1 1 2 2+ 3+ 6 7 19+
Cw*1701 1+ 1+ 13+ 9 11 13 13 14 13+ 14 14 14+ 14+ 17 6 6 6 7 7 7+ 7+ 7+ 7+ 7+ 7+ 8 8 8 9 15 4 6 6+ 13
Cw*1802 8 11 11+ 4 17+ 15 8+ 9+ 8+ 10 9+ 9+ 9 9 9 16+ 1+ 1+ 2 2 2 2+ 2 2 3+ 3+ 3+ 19+ 20+ 10+ 4 24+ 3 3+ 4 6+
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HLA-EG as an immunogen is strikingly different from HLA-ER in eliciting HLA-Ia and Ib reactivity. Ten mAbs (Group G-VIII) showed reactivity only with HLA-F of HLA-Ib isoforms and with almost all alleles of HLA-Ia isoforms. TFL-235 showed remarkably high reactivity with all HLA-A (n = 31), HLA-B (n = 50), and HLA-C (n = 16) alleles tested, whereas the mAb failed to react with HLA-G. On the other hand, Group G-IX, subgroup A (eight mAbs) showed weak reactivity (MFIs 1 K to 2+ K) with HLA-G, but consistently reacted strongly with HLA-A*1101 and weakly with HLA-A*2402, in addition to reacting with several alleles of HLA-B and HLA-C. Group G-IX, subgroup B (three mAbs) reacted strongly with HLA-G (MFIs: 11–14 K) in addition to moderate reactivity with several alleles of HLA-Ia isoforms. The subgroup C (five mAbs) reacted well with HLA-G, but showed very strong reactivity (MFI 8 K–19 K) with several alleles of HLA-B alleles in addition to their reactivity to several HLA-A and HLA-C alleles. Another striking feature of this group is that four of the five mAbs showed MFI of 10 K to 24 K with C*1802.

Group G-X (four mAbs) is unique in that these mAbs reacted with HLA-F and HLA-G in addition to alleles of all the three HLA-Ia isoforms. TFL-232 showed high reactivity to HLA-G (MFI: 21 K). In addition to its reactivity with A*1101 and A*3402, it showed reactivity with HLA-B and HLA-C. The other three mAbs, while showing positivity to HLA-F and HLA-G, reacted reasonably well with alleles of the three HLA-Ia isoforms. The mAb TFL-198 is strikingly different in that it showed weak reactivity with HLA-G (MFI: 0+), moderate reactivity with most of the HLA-A alleles and very strong reactivity with several of HLA-B and HLA-C alleles. None of these three mAbs of G-X met the amazing polyreactivity of R-X mAb TFL-006, specifically to open conformers (Table 10).

Table 10.

TFL-006 fails to recognize HLA antigens coated on LIFECODES beadsets as they contain only intact HLA (closed conformers).

HLA-I Reactivity with TFL-006 (MFI) at 20 mG/mL *
HLA-I LABSCreen LIFECODES HLA-I LABSCreen LIFECODES
(Lot # 10) (Lot # 11) 3005613 (Lot # 10) (Lot # 11) 3005613
A*01:01 933 852 0 B*07:02 862 670 0
A*02:01 339 542 0 B*08:01 1226 1204 0
A*02:03 1018 156 0 B*13:01 n/a 675
A*02:06 n/a 616 B*13:02 2514 4923 0
A*03:01 193 210 0 B*14:01 7805 1983 0
A*11:01 4782 3900 0 B*14:02 1831 197 0
A*11:02 537 321 0 B*15:01 335 2541 0
A*23:01 133 125 0 B*15:02 1935 809 0
A*24:02 716 580 0 B*15:03 1822 1146 0
A*24:03 2516 1042 0 B*15:10 n/a 579
A*25:01 194 349 0 B*15:11 n/a 1658
A*26:01 2221 2273 0 B*15:12 770 1875 0
A*29:01 1017 1667 0 B*15:13 3135 2437 0
A*29:02 778 715 0 B*15:16 3076 882 0
A*30:01 1496 1243 0 B*18:01 3096 2891 0
A*30:02 n/a 728 B*27:05 634 3252 0
A*31:01 396 687 0 B*27:08 1659 2095 0
A*32:01 515 341 0 B*35:01 6128 517 0
A*33:01 1038 574 0 B*37:01 2650 4352 0
A*33:03 554 1039 0 B*38:01 2521 2425 0
A*34:01 n/a 3002 B*39:01 704 4447 0
A*34:02 1535 1165 0 B*40:01 3429 330 0
A*36:01 1353 941 0 B*40:02 2697 3354 0
A*43:01 2479 2768 0 B*40:06 n/a 1869
A*66:01 1886 2145 0 B*41:01 3739 1364 0
A*66:02 1454 1327 0 B*42:01 347 957 0
A*68:01 713 454 0 B*44:02 3650 892 0
A*68:02 1185 578 0 B*44:03 1829 2516 0
A*69:01 3128 1786 0 B*45:01 1736 2568 0
A*74:01 652 603 0 B*46:01 3572 1415 0
A*80:01 3132 3758 0 B*47:01 2152 1859 0
B*48:01 3262 2418 0
B*49:01 1554 3042 0
0 B*50:01 1799 4246 0
C*01:02 4066 2820 0 B*51:01 2461 2413 0
C*02:02 7446 3403 B*51:02 n/a 696
C*03:02 n/a 6614 0 B*52:01 2146 1745 0
C*03:03 2458 3071 0 B*53:01 5442 695 0
C*03:04 4504 8167 0 B*54:01 1662 2427 0
C*04:01 3337 4182 0 B*55:01 2519 4867 0
C*05:01 9124 2567 0 B*56:01 3662 3084 0
C*06:02 5644 5794 0 B*57:01 2089 141 0
C*07:02 8702 1689 0 B*57:03 n/a 1878
C*08:01 6090 4479 B*58:01 5268 3190 0
C*12:03 n/a 6731 0 B*59:01 3553 1278 0
C*14:02 3937 3627 0 B*67:01 406 3520 0
C*15:02 4465 4651 0 B*73:01 1423 3565 0
C*16:01 4648 2706 0 B*78:01 2996 5271 0
C*17:01 8296 5760 0 B*81:01 1525 2446 0
C*18:02 n/a 829 B*82:02 n/a 1959
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Note: TFL-006 binds to LABScreen beadsets, wherein β2-m-free HLA HCs (open conformers) that are admixed with intact HLA (closed conformers). Alleles in bold are antigens unique to LABScreen, * MFI less than 30 are indicated as 0.

3.7. HLA-I Reactivity in Human Sera

3.7.1. Non-Alloimmunized Males’ Sera Reactivity to HLA-I Alleles Reflects the Profiles of Some of the HLA-E mAbs

Non-alloimmunized males’ sera showing high positivity for anti-HLA-E IgG antibodies were examined for IgG HLA-I reactivity (Table 11). The following aspects of HLA-I reactivity resemble that of HLA-I reactivity of HLA-E mAbs: (1) HLA-A reactivity of 8 out of 11 sera were low or none (1 to 3 alleles). The highest frequency for HLA-A locus specificities is for A*2402; (2) All sera showed high reactivity for HLA-C; (3) The highest HLA-B frequency was observed for B*4006 = B* 3701 > B*8201; (4) The HLA antibody profile of male donor TJ was strikingly similar to that observed in Group R-IV subgroup B mAbs, presented in Table 4; (5) Similarly, the serum antibody profile of HR was somewhat similar to Group R-IV subgroup E, though HLA-C reactivity was high; and (6) the serum Abs of two male donors (VJ and HO) were similar to the profiles of the mAbs G-VII subgroups C and D.

Table 11.

HLA-Ia reactivity of antibodies of non-alloimmunized males.

HLA Alleles ID of Non-Alloimmunized Healthy Males
Values Expressed as Mean Fluorescent Intensity (MFI)
TJ HR ME RC TP CM MR TS NR VJ HO
HLA-A Reactivity A*1101 769 898
A*2301 609 972
A*2402 616 726 869 632 1099 738 784 1021
A*2403 557 599
A*2501 906
A*2601 835 613 1094
A*2901 1013
A*2902 715 2224
A*3001 540
A*3101 578
A*3201 539 1511
A*3301 502 1260
A*3303 712
A*3401 958 710 1867 749 2070
A*3601
A*4301 779 562 1660
A*6601 2535 1387
A*6602 1830 1382
A*6802 818 946
A*6901 628 618 581 523
A*7401 716
A*8001 595 919
HLA-B Reactivity B*1301 556 564 969 737 722 1025
B*1302 582 594
B*1401 524 518 704 506 602 976 830 944 911
B*1402 552 556 648 624
B*1502 618 523 573
B*1503 535
B*1510 578 1089
B*1511 592 634 703 667 862 1113 882 1014 1268
B*1512 562 1923 564 2484 501
B*1513 507 745 757 713 808
B*1516 596 572 571 1009 750 1341 2200
B*3501 727
B*3701 748 550 1374 881 1135 725 5103 1203 1215 1901
B*3801 559
B*4001 739
B*4006 746 976 932 578 1196 1586 1288 1211 1749
B*4402 576 2027 546 1375 713 688 779
B*4403 1777
B*4501 673 2044 506
B*4601 2821 442 941 741 549 558 871 842
B*4701 929
B*4801 697 609 648 537
B*5101 626 658 687 718
B*5102 525 656 748
B*5201 836 565 672 1281
B*5301 816 693 748 842
B*5401 565 627
B*5501 548 541 604
B*5601 537 1197
B*5701
B*5703 632 829
B*5801 659 669 653 960 885 934 1193
B*5901 591 552 658 675 887 900
B*6701
B*7301 583
B*7801 812 574 680 648
B*8101 807 543
B*8201 799 942 3259 989 1041 994 993
HLA-C Reactivity C*0102 619 1301 676 1421 1588 1451 1682 1796 1876 1774 2602
C*0202 680 1314 877 1286 1823 1032 1758 2080 1833 1824 2508
C*0302 549 1397 759 1226 1280 886 1291 1913 1897 1351 1893
C*0303 1442 607 937 1073 977 1376 1417 1151 1619
C*0304 620 1438 689 1211 1395 668 1309 2017 1743 1487 2209
C*0401 907 1602 1299 2038 2427 1113 2130 2815 2610 2148 4036
C*0501 628 1149 1078 1268 1406 659 1814 2123 1851 1529 1762
C*0602 1326 2657 1445 2520 3017 1572 2675 3410 2743 2945 2819
C*0702 1120 1649 1520 2276 2748 1426 2801 3562 3441 2760 4098
C*0801 855 926 1027 1117 1423 1274 1281 1514
C*1203 550 920 672 1187 1402 1207 1430 1631 1502 1590 1820
C*1402 792 1609 1100 1778 2045 1155 2019 2669 2386 2023 3444
C*1502 701 509 915 1119 1086 1025 1348 1122 1364 1489
C*1601 652 1249 1064 1362 1596 877 1510 2044 1731 2038 2093
C*1701 720 1396 1043 1719 2197 763 1735 2683 2120 1792 1885
C*1802 1175 1878 1634 2135 2386 1549 2605 3014 2932 2463 2695
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Note: Sera were purified using protein-G column and elute-2 alone (1/10 dilution) used for monitoring the HLA-I reactivity using Luminex Single Antigen Bead Assay.

3.7.2. Pattern of HLA-I Reactivity in Melanoma Patients before and after Immunizing with Autologous IFN-gamma Activated Tumor Cells Reflects HLA-E mAb Profiles

Anti-HLA-E Abs in normal human sera may be responsible for antibody reactivity to allo-HLA-Ia alleles. This provides support to the hypothesis that HLA-E is immunogenic in males. HLA-E and HLA-E HCs are more prevalent on tumor cells, including melanoma, as evidenced by immunostaining with the monospecific mAbs TFL-033 and the polyreactive mAb MEM-E/02, which bind to the cryptic epitopes exposed on the heavy chain of HLA-E [37]. It has been established that IFN-gamma significantly increased the surface expression of HLA-E and the shedding of soluble HLA-E by melanoma tumor cells [42,43]. To assess the immunogenicity of tumor associated HLA-E in cancer patients, specifically in stage III and/or stage IV melanoma patients, sera obtained at weeks 0 and 4 after immunizing with the autologous tumor cells activated by IFN-gamma, were examined for the presence of both HLA-E antibodies and HLA-class Ia allelic reactivity. Table 12 documents that autologous tumor cell vaccination augmented the HLA-E IgG antibodies in the sera and concomitant reactivity against several allo-HLA-Ia alleles. The allo-HLA-Ia reactivity of HLA-E Abs in the vaccine sera was confirmed by inhibiting the HLA-E and HLA-Ia reactivity of the sera with peptide sequences shared between HLA-E and HLA-Ia alleles [41].

Table 12.

An unaltered profile of HLA-Ia reactivity in the sera of six melanoma (Stage IV) patients before and 4 weeks after administering autologous melanoma cell vaccine (tumor cells treated with IFNγ were co-cultured with autologous dendritic cells in 1:1 ratio).

Mean Fluorescent Intensity (MFI) of Anti-HLA-Ia IgG Antibodies in Melanoma Patients before and 4 Weeks after Autologus Whole Cell Cancer Vaccine
Patient ID Before and after 4 Wks Post vaccine ER*0101 HLA-B Reactivity HLA-C Reactivity
B*0702 B*1301 B*1401 B*1512 B*1516 B*2705 B*2705 B*2708 B*3701 B*4001 B*4002 B*4006 B*4201 B*4402 B*4701 B*5401 B*5501 B*5601 B*5701 B*5703 B*6701 B*8101 B*8201 C*0101 C*0202 C*0302 C*0303 C*0304 C*0401 C*0501 C*0602 C*0702 C*0801 C*1203 C*1402 C*1502 C*1601 C*1701 C*1801
HCC-1763 PreVax 550 590
PostVax 830 1330
HCC-1766 PreVax 3140
PostVax 3830 700
HCC-1937 PreVax 3710
PostVax 4040 570 940 650 550 670 1107
HCC-1896 PreVax 3710 3340 1490 1740 1080 980 1430 1150 820 1210 3250 1170
PostVax 4520 3610 1720 1820 990 1030 1480 1190 830 1240 3460 1210
HCC-2030 PreVax 1650 540 12,330 1180 540 530 8760 540 570 3460 6180 540 6140 750 750 930 500 700 2180 950 1500 660 600 1830 910
PostVax 8620 640 1230 12,700 2240 790 760 9040 830 950 3190 5520 670 5340 1580 1340 1901 1030 690 1330 2560 1690 2940 650 1240 630 2730 1300
HCC-1957 PreVax 3870 540 640 1250 570 1080 1020 540 770 1150 580 560
PostVax 4990 1050 1370 2550 1170 2250 1933 700 1620 880 1370 2040 850 850
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3.7.3. Pattern of HLA-I Reactivity of HLA-Sensitized ESKD Patients Waiting for Transplantation

In the end-stage organ disease patients with high levels of preformed anti-HLA antibodies (highly sensitized), transplant rates are significantly reduced because of the increased rejection risk [44]. The highly sensitized patient is destined to remain on the waiting list for extended periods on dialysis, an added risk factor which may decrease both graft and patient survival [45,46]. It is often construed that these antibodies result from exposure to non-self HLA antigens, usually from previous transplants, blood transfusions, and/or pregnancies [47]. Examination of the HLA-reactivity in 10 patients with ESKD, was presented in Table 13, it was noted that the profile of the HLA-I reactivity is similar but not identical to any specific anti-HLA-E mAbs. One patient (11DM) showed reactivity to HLA-A* 1101, and several other A and B alleles, similar to anti-HLA-E mAbs (Groups G-VII E and G). Several patients also showed several similarities in the reactivity of HLA-A and B alleles with anti-HLA-E mAbs (Groups G-VII). These observations suggest that the varying reactivity observed in the patients may be a result of antibody response to HLA-E and possibly to other HLA-Ib alleles with high prevalence of open conformers, such as that of HLA-F and HLA-G.

Table 13.

Profiles of HLA-Ia reactivity of sera of HLA-sensitized ESKD patients waiting for transplantation.

Patient ID 10DM 17LE 12DM 20HN 5DM 19HN 1DM 11DM
No of Alleles HLA-A 1 9 3 6 10 2 10 22
HLA-B 11 4 15 14 10 16 12 4
HLA-C 2 1 1 0 0 2 2 0
HLA-A Reactivity A*01:01 9470 1694 1255 14,961 3937
A*02:01 1364 1894 872
A*02:03 1034 1809 690
A*03:01 5139
A*11:01 4621
A*11:02 4519
A*23:01 1783 1521 13,902 15,118
A*24:02 1985 1527 13,190 14,810
A*24:03 754 1395 11,895 689
A*25:01 1010 4204
A*26:01 4826
A*29:02 1634 3543 1090 3014 1596 4622
A*30:01 4679
A*31:01 3733
A*32:01 4744
A*33:01 1831 4431
A*33:03 5485
A*34:02 7463
A*36:01 2587 5234
A*43:01 661 3826
A*66:01 6275
A*66:02 6318
A*68:01 1980 537 5555
A*68:02 823 2054 619 6220
A*69:01 639 1557 5415
A*74:01 5083
A*80:01 537 2209 3355 1010
HLA-B Reactivity B*07:02 2638 5808 2593
B*08:01 1460
B*15:12 517 1509 1304
B*15:16 2057 4253 3698
B*27:05 932 1650 591 2757
B*27:08 1459 1232
B*37:01 808 1495 2849 1095
B*38:01 1073 1405 2923 1204
B*39:01 917 1559
B*40:01 2573 4726 583 549
B*40:02 841 710 982 17,106 17,617
B*41:01 1288 1142 15,827 17,864
B*42:01 1807 3532 776 1469
B*44:02 796 549 1373 2117
B*44:03 1888 1532 2212
B*46:01 613 998
B*47:01 1447 3431
B*48:01 1612 3014
B*49:01 1248 2768
B*50:01 1488 1351
B*51:01 589 2013
B*52:01 1606 3103
B*53:01 611 1740 1235
B*54:01 760 1909 1375
B*55:01 552 1666 1493
B*56:01 1260 1578
B*57:01 2252 4064 503
B*59:01 2527 5685 2546
B*67:01 1198 1015 2872 17,248 17,353
HLA-C C*03:03 611
C*15:02 1283 1630 1300
C*17:01 1048 1365 2695 997
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4. Discussion

4.1. Immunogenicity of HLA HCs (Open Conformers) versus Intact HLA (Closed Conformers)

A number of investigators [5,6,7,8,9,10,11,12,13,14,15] have demonstrated that HLA-I molecules not only occur as heterodimers on the cell surface, but also occur as monomers or the β2m-free HCs (open conformers). The HLA-I HCs may be involved in functions other than antigen presentation [15,16,17,18,19,48]. The open conformers of HLA alleles expose amino acid sequences, which are cryptic in intact HLA. Therefore, there is a possibility that the immune system may recognize immunogenic domains on the open conformers as “non-self”. Moreover, the cell surface expression of the open conformers is augmented by inflammation, infection, trauma, and malignancy [5,6,7,8,9,10,11,12,13,14,15,19,48]. The breadth and strength of the HLA antibodies, whether directed against HC open conformers or closed conformers, are augmented by the inflammation stimulated by end-stage organ diseases present in transplant patients, as well as by infection, trauma, malignancy, and the transplantation process itself [49,50].

Following peptide presentation or shedding by trimeric closed conformers, the β2m gets separated from the HLA-I HCs. The dissociated HCs, as well as the naturally occurring open conformer HCs are cleaved from the cell surface by calcium-dependent and zinc-containing matrix metalloproteinases (MMPs) [2] and are shed first into the microenvironment and then into the circulation. Upon shedding of HCs, the HC may get deglycosylated and degraded by proteases. Even if we immunize intact HLA in a human or animal model, it will soon be dissociated and degraded into the circulation. Depending on the duration of the degradation, the HCs that can be recognized by the immune cells to elicit antibody responses. The time frame of degradation may vary with the conformational orientation of the amino acid sequences. The partitioning of the diversified groups and subgroups of mAbs against the HCs of HLA-E*0101 and HLA-E*01:03 outlined above supports the above contention.

The exposure of the cryptic sequences and their fragments may elicit an immune response and result in antibody formation against several such epitopes. One has to visualize the HC of a closed conformer and compare it with that of an open conformer. What is common is that the α1 and α2 domains in the immediate vicinity of the peptide groove are exposed in both closed and open conformers (Figure 3). The amino acids between 80 and 140 of the HC are cryptic in closed conformers. No antibody can recognize them in intact HLA (closed conformers) due to the presence of β2m. However, when the HC is devoid of β2m, as in open conformers these sequences are exposed for immune recognition. That explains the ramification of the mAbs reacting to different HLA-I isoforms. One may argue that the diversity of the mAbs is due to immunization of human HLA-E HC molecules in a non-human (mouse) model. Amazingly (as shown in Figure 1), the amino acid sequence of the murine equivalent of HLA-E, namely Qa1b, shows similarity to HLA-E comparable to that of HLA-F and HLA-G, particularly in the α1 and α2 domains [51]. There is not much variation in the α3 domain among HLA-I, for this domain is highly shared among all HLA-I isoforms.

4.2. HLA-E mAbs Recognizing Private Epitopes

The shed HCs expose two categories of amino acid sequences to be recognized by the immune system. The first category is referred to as “private epitopes”, the sequences specific or unique for an individual isoform or allele. They are the monospecific epitopes. Only the anti-HLA mAbs formed against the monospecific epitopes are reliable for immunodiagnosis of specific HLA-isomers or alleles. Twenty mAbs developed against HLA-ER and five mAbs developed against HLA-E*01:03 (HLA-EG) recognized only HLA-E and not HLA-F or HLA-G or any of the isoforms of HLA-Ia. The monospecific or private epitopes are located in the α1 and α2 domains surrounding the peptide groove (Figure 3). To further clarify, it may be stated that the sequences 65RSARDTA71 and 143SEQKSNDASE152 are found only in HLA-E alleles (e.g., HLA-ER and HLA-EG) and not in any other isoforms (Figure 2 and Figure 3). We have examined more than 500 HLA-A alleles, 800 HLA-B alleles, 280 HLA-C alleles, several HLA-F and HLA-G alleles to ascertain whether 65RSARDTA71 and 143SEQKSNDASE152 are indeed monospecific epitopes or “truly private epitopes” specific for all HLA-E alleles or only HLA-E alleles. Most importantly, we have synthesized the sequences 65RSARDTA71 and 143SEQKSNDASE152 and tested whether they block the binding of one of the monospecific mAbs (TFL-033) to HLA-E coated beads. The dosimetric inhibition of binding confirms the specificity of the epitopes [37]. The sequence between amino acids 65 and 71 differs markedly as follows: 65RSARDTA71 (found only in HLA-E); whereas in HLA-F, it is 65NTKAHA71; in HLA-G, it is 65YAKAHA71; in all C alleles, it is 65KYKRQA71; in B*1501, it is 65ISKTNT71; in B*1510, it is 65ICKTNT71; and in B*1516, it is 65NMKASA71. An examination of the amino acid sequences in this region of the peptide groove of both α1 and α2 sequences reveals the diversity of private epitopes.

4.3. HLA-E mAbs Recognizing Public Epitopes

The other category of HLA-E epitopes is called “public epitopes”, shared by HLA-E and other HLA-Ia and HLA-Ib isoforms. In a thought-provoking review, Tambur and Class attempted to clarify “that every HLA antigen has its own unique combination of epitopes, but at the same time, many of these epitopes are shared with some other HLA antigens” [52]. However, Figure 2A–C illustrates that “many of these epitopes are not just shared with some other HLA antigens” but shared with almost all HLA-I antigens. Figure 2D points out that 117AYDGKDY123 is found in 491 HLA-A, 831 HLA-B and 271 HLA-C alleles, 126LNEDLRSWTA135 in the α2 domain of 239 HLA-A, 219 HLA-B and 261 HLA-C alleles, whereas 137DTAAQI142 is shared by 824 HLA-B and 248 HLA-C alleles; however, the sequence is altered in HLA-A as 137DMAAQI142. Indeed, the sequences 117AYDGKDY123, 126LNEDLRSWTA135 befit the definition of shared or “public epitopes” (see Figure 1 and Figure 2). In the HLA-I α3-domain, a major fraction of the common amino acid sequences are shared by almost all other HLA-I molecules. The public epitopes in the α1 and α2 domain are always masked by β2-m in intact HLA. The β2-m causes steric hindrance for IgG binding; however, these public epitopes are well exposed in open conformers.

4.4. Ramifications of the HLA-I Allelic Reactivity of Anti-HLA-E*01:01 and Anti-HLA-E*01:03 mAbs

An interesting finding is that the two HLA-E alleles used for immunization, namely, HLA-E*01:01 (HLA-ER) and HLA-E*01:03 (HLA-EG) differ by one amino acid substitution at position 107, resulting in an arginine in HLA-E*01:01 (HLA-ER) and glycine in HLA-E*01:03 (HLA-EG) and there are resulting ramifications on HLA-I allelic reactivity. In contrast to HLA-ER, HLA-EG has higher affinity to peptides, higher surface expression, and higher thermal stability, and it is more ancient than HLA-ER, though both alleles are presented in human populations in nearly equal frequencies [53]. Interestingly the ramification of the groups of mAbs generated by the two alleles shows some similarities and several striking differences in the pattern of their HLA-I allelic recognition (Figure 7).

Figure 7.

Figure 7

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Diagrammatic illustration of the ramification of HLA-I reactivity of the anti-HLA-E*0101 and anti-HLA-E*0103 mAbs.

A noteworthy similarity of mAbs developed against both alleles is that they recognize HLA-A*1101 exclusively. In fact, we have categorized mAbs negative (R-IV (30 mAbs) and G-V (4 mAbs)) and positive for HLA-A*1101 (Group R-V (68 mAbs) and G-VI (15 mAbs)). Besides one of the HLA-Ib reactive mAb from HLA-ER recognized HLA-A*1101 exclusively.

4.5. Many HLA-E mAbs May Recognize Conformationally Altered Sequences

Although all HLA isomers possess both monospecific (private epitopes) and shared (public epitopes) amino acid sequences, the conformational variations of the quaternary structure do not retain all sequences in a linear format. Both Figure 3 and Figure 4 illustrate this point clearly. Consequently, monospecific and polyreactive antibodies do not necessarily recognize linear sequences of the monospecific and shared amino acid sequences. There may be subtle variations introduced by folding due to differences in the other neighboring amino acids. This is based on the following findings:

  • (a)

    The only difference in the amino acid sequences of HLA-EG107 and HLA-ER107 is the presence of glycine in the former, and arginine in the later, at position 107. Interestingly, of the total mAbs obtained after immunizing a single mouse with HLA-EG107 or two mice with HLA-ER107, eight mAbs of HLA-EG recognized HLA-F, while only one mAb of HLA-ER recognized HLA-F. While eight mAbs of HLA-EG recognized HLA-G, only one mAb of HLA-ER recognized HLA-G. These observations suggest that the presence of glycine at 107 in HLA-E may impact the conformational orientation of the HLA-E to elicit mAbs reactive to HLA-F and HLA-G.

  • (b)

    The most striking aspect in their allelic recognition is that almost all mAbs (132 out of 133) developed against HLA-E*01:01 (HLA-ER) recognized HLA-B*4006 (Figure 6 and Figure 7). Only 17 anti- HLA-E*01:03 (HLA-EG) mAbs showed low reactivity (<4K) to B*4006. Specific recognition of B*4006 by mAbs belonging to groups R-III and R-IV (Table 4) clearly points out the unique exposure of the conformation of B*4006 comparable to HLA-ER107, that is not observed with Group G, which is possibly due to the presence of glycine at position 107.

  • (c)

    Similarly, HLA-B*1511 is better recognized by the mAbs of HLA-E*01:03 (HLA-EG) than those developed from HLA-ER. The specific recognition of B*1511 by mAbs belonging to groups G-V and G-VI (Table 7) points out the unique exposure of the conformation of B*5011 is comparable to HLA-EG107, in contrast to that of HLA-ER107.

  • (d)

    HLA-C*1802 was recognized by all mAbs of HLA-ER, whereas many mAbs raised against HLA-EG failed to recognize the same allele. Figure 7 diagrammatically illustrates the trends of HLA-I reactivity of the anti-HLA-E*0101 and anti-HLA-E*0103 mAbs.

  • (e)

    Group R-IV (Table 4) anti-HLA-E mAbs bind to HLA-B and C but do not bind to any of the HLA-A alleles, possibly due to the presence of 137DTAAQI142 found in HLA-E/-G/-B and –C), whereas in HLA-A, it is 137DTMAAQI142 (Figure 2). The T138 is replaced by M138. Figure 4 clearly shows the location of DTAAQI, in which only DT and QI are exposed during folding. Replacement of “T’ with “M” alters the immune recognition by the 30 mAbs in R-V.

  • (f)

    Group R-IV mAbs do not bind to HLA-F, for DTAAQI is modified as 137DTVAQI142 in HLA-F.

  • (g)

    Group R-IV mAbs also do not bind to HLA-G due to change in the closely associated neighboring amino acid of DTAAQI, as follows: in HLA-E, the amino acid preceding DTAAQI is V, whereas in HLA-F and HLA-G, the V is replaced with A (for details see Figure 1).

  • (h)

    In 137DTAAQI142, the amino acid in the 136thposition and in the 143rdposition vary markedly, as seen in Figure 2. These changes may impact the exposure of the sequences. Such specific alterations can impact immune recognition by the mAbs.

4.6. Relevance of mAbs to Serum Antibodies of Normal Healthy Humans

Several allo-HLA antibodies are known to occur naturally in the sera of both men and women that have not been alloimmunized by transfusion, prior transplantation, or pregnancy [24,25,26,27,28,29]. Morales–Buenrostro et al. [29] demonstrated the specificities and incidence of the allo-HLA-I Abs in non-alloimmunized males. Neither the origin nor the cause of anti-HLA Abs occurring in healthy individuals have been established. The observations made on the non-alloimmunized sera after purifying with protein-G column, reported in this study, establish the prevalence of anti-HLA-Ia IgG antibodies in the sera of normal, non-alloimmunized individuals. These sera had anti-HLA-E IgG antibodies. It was further observed that the IgG antibodies in the sera recognized several HLA-Ia alleles. The most prevalent alleles recognized by the healthy male sera are B*8201 (n = 13), C*0302 (n = 13), C*0102 (n = 11), B*0702 (n = 10), C* 0303 (n = 9), B*5601 (n = 9), B*4402 (n = 8), C*1701 (n = 8), B*1502 (n = 7), B* 1512 (n = 7), C*1502 (n = 7), C*1203 (n = 7), C*0202 (n = 7), B*3701 (n = 6), C*0501 (n = 6), and C*1802 (n = 6). Interestingly most of these sera reacted with HLA-A alleles as observed with anti-HLA-ER and anti-HLA-EG in Table 4 and Table 6.

In an earlier investigation, the presence of HLA-E and HLA-Ia antibodies in normal sera was validated by using commercially available purified human IgG, obtained from Southern Biotek (Birmingham, AL, USA) [28]. These commercial preparations are made from sera of 4 or 5 normal and healthy volunteers, and the purified normal sera IgG are used as a standard for immunoassays. The analysis revealed reactivity of the commercial human IgG to HLA-B (HLA-B*3701) and six other HLA-C alleles (C*0202, C*0501, C*0602, C*0702, C*1601, and C*1802). The alleles of HLA-Ia exhibiting reactivity to the purified human IgG are strikingly similar to HLA-Ia reactivity observed in normal human sera as well as by anti–HLA-ER mAbs in Group R-IV-C (mAb TFL-093). Based on these findings, we infer that most of the anti-HLA antibodies observed in non-alloimmunized males could be autoantibodies generated in the individual against one’s own HLA open conformers, such as that of HLA-E. Of course, there could be a few monospecific mAbs, directed against private or monospecific epitopes. These human HLA-I antibodies are the consequence of shedding of self HLA open conformers (HCs of HLA-A, HLA-B, HLA-C, HLA-E, HLA-F, and HLA-G).

The above inference is also applicable to intravenous immunoglobulin (IVIg), a pharmaceutical preparation of plasma-derived human immunoglobulin G (IgG). It is fractionated from the pooled plasma of 1000 to 60,000 blood donors, including non-alloimmunized donors. Similar to the normal human sera, different IVIg preparations showed a wide variety of reactivity to classic HLA-Ia [54,55,56]. Interestingly, in the previous report, we have ascertained whether the immunoreactivity to HLA-Ia alleles found in IVIg is due to the anti–HLA-E Abs present in IVIg. We have adsorbed out the anti–HLA-E Abs with Sephadex gel conjugated with recombinant HLA-E, then tested for HLA-E and HLA-Ia reactivity. As expected, IVIg immunoreactivity to both HLA-E and HLA-Ia is lost after the adsorbing-out process (see Figure 6A,B in [54]). This previous finding is well supported by the present investigation of anti-HLA-E mAbs.

4.7. HLA-E Polyreactive mAbs (TFL-006 and TFL007) Mimic Immunoreactivity of IVIg

Therapeutically, IVIg is administered to reduce allo-HLA antibodies pre- and post-transplantation, but the mechanism of reduction remains unclear. We have compared the efficacy of IVIg and the Group R-X mAb TFL-007, which mimics the HLA-Ia and HLA-Ib reactivity of IVIg in suppressing both antibody production [57] and blastogenesis and proliferation of CD4+ T cells [58]. The mAb-007 significantly suppressed the allo-HLA class-II IgG produced by the B cells, and this suppression was far superior to that by IVIg [55,56]. These findings were confirmed with HLA-I antibody secreted by an immortalized B cell line, developed from the blood of another alloimmunized woman [57]. The binding of the mAb TFL-007 to peptide sequences shared (i.e., shared epitopes) between HLA-Ib and β2m-free HLA HCs (open conformers) on the cell surface of B cells may act as a ligand that induces suppression of IgG production of activated B memory cells.

4.8. Profiles of HLA-Ia Reactivity of Anti-HLA-E IgG in Melanoma Patients Reflect the HLA-I Reactivity of Some of the Anti-HLA-E mAbs

Melanoma patients’ sera contain IgG antibodies against HLA-E as well as HLA-B and HLA-C. Interestingly, the HLA-I reactivity in the patients’ sera strongly recalls the pattern observed in Groups R-IV and G-V in that the sera are totally negative for HLA-A alleles. Indeed, the vaccination with autologous tumor cells augmented the MFIs of HLA-E and several other HLA-Ia alleles. However, the pattern of allo-HLA-Ia allelic reactivity of the post-vaccination sera is identical to that observed in pre-immune sera. No new allo-HLA-Ia allelic reactivity was observed in any of the patients after immunization with autologous tumor cell vaccine.

4.9. Profiles of HLA-Ia Reactivity of Sensitized Sera of ESKD Patients Reflect the HLA-I Reactivity of the Anti-HLA-E mAbs

The HLA-I reactivity of sensitized patients waiting for donor organs is strikingly different from the normal and melanoma patients’ sera. Sensitized patients showed antibodies for HLA-A*0101, HLA-A*0201, HLA-A*0203, and HLA-A*0301. Among mAbs raised against HLA-ER and HLA-EG, two mAbs in Group R-X (TFL-006 and TFL-007), two mAbs in Group G-VII (mAbs TFL-171 and TFL-172), and one in G-GVIII (TFL-235) showed the reactivity to all these alleles. These findings emphasize that the HLA sensitization, as monitored by measuring the strength of HLA antibodies, is a consequence of immune recognition of HLA open conformer on the surface of cells activated by inflammation, infection, and traumas. Furthermore, these findings can be extended to post-transplantation since transplant surgery per se is an inflammation inducer leading to immune recognition of HLA open conformers [49].

5. Conclusions

The ramification of HLA-Ia reactivity of the diverse mAbs generated by immunization of the HCs of one HLA-E allele points out the complexity of HLA-I antibodies. This study emphasizes the need to undertake such studies with HLA-F and HLA-G alleles for the purposes of both comparison and elucidation of the patterns of diversity. Correlating the diverse HLA-Ia and HLA-Ib reactivity patterns of serum antibodies after infection, inflammation, autoimmune diseases, and in different cancers and end-stage organ diseases may provide clues to the primary immunogen and the source of the immunogen involved in these diseases. This would be particularly valuable for patients with various cancers and end-stage organ diseases.

Poet William Wordsworth once said that “one impulse from a vernal wood may teach you more of man, of moral evil and of good, than all the sages can.” We conclude from this investigation that “one heavy chain of an HLA exposed to an immune system may reveal the origin and ramifications of human HLA antibodies, their good and bad potentials, than all the transplant and cancer clinicians can envisage”.

Acknowledgments

M.H.R. is indebted to the late Paul Ichiro Terasaki for his invaluable discussions during the course of this investigation. We sincerely thank Vadim Jucaud and Tho Pham, research associates at Terasaki Foundation laboratory for meticulously carrying out the SAB assays. Finally, we want thank Mark Terasaki for his constant support and encouragement. The mAbs TFL-033, TFL-034, TFL-073, and TFL-07 4 (monospecific mAbs), and TFL-006 and TFL-007 were patented. The mAbs TFL-033 and TFL-006 were deposited with ATCC. The rest of the mAbs are cryopreserved at Terasaki Research Institute, Los Angeles and monitored by Vadim Jucaud and Tho Pham.

Abbreviations

Abs: antibodies; Ag: antigen; β2m: beta-2 microglobulin; HLA: human leukocyte antigens; HC: heavy chain; mAb: monoclonal antibodies; MFI: mean fluorescent intensity; MMP: matrix metalloproteinases; NK cells: natural killer cells; SAB: single antigen bead.

Author Contributions

Conceptualization, M.H.R. and N.M.R.; methodology, M.H.R. and M.H.R.; software, M.H.R.; validation, M.H.R., N.M.R. and S.R.S.; formal analysis, S.R.S., E.J.F. and F.E.H.; investigation, M.H.R.; resources, M.H.R.; data curation, M.H.R.; writing—original draft preparation, M.H.R.; writing—review and editing, M.H.R., E.J.F., S.R.S. and F.E.H.; visualization, M.H.R.; supervision, M.H.R.; project administration, M.H.R.; funding acquisition, M.H.R. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

This study primarily involves mice and the immunization was carried out at One Lambda Inc. (Canoga Park, Los Angeles, CA, USA) and does not require Institutional Review board statement.

Informed Consent Statement

Informed consent was obtained from all subjects involved in this study. For each cohort the details of informed consent statement is provided in Section 2.3, Section 2.4 and Section 2.5.

Data Availability Statement

The original data is available at Terasaki Foundation Laboratory.

Conflicts of Interest

Senthamil R. Selvan is is an employee of Tetracore Inc. The authors declare no conflicts of interest.

Footnotes

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The original data is available at Terasaki Foundation Laboratory.

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