Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 10;11(6):955. doi: 10.3390/cells11060955

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Palmitic acid exposure of dual-stressed HPdLFs resulted in decreased H3K27 trimethylation near IL10 promoter regions associated with decreased IL10 expression. (a) Quantitative expression analysis of IL10 in human periodontal ligament fibroblasts (HPdLFs) exposed to palmitic acid (PA) and simultaneously stimulated with compressive force (CF) and P. gingivalis LPS after treatment with 1.00 µM UNC1999 compared to DMSO-treated BSA+LPS+CF control. (b) DNA primer locations in the IL10 gene locus indicating promoter-associated pairs (#1, #2 and #3) and a non-promoter-associated pair (#4). (c) Quantitative analysis of the associations of H3K27me3 with specific IL10 gene regions shown in (b) for dual-stimulated HPdLFs exposed to PA after UNC1999 treatment in comparison to the respective controls. Data were normalized to the sample input and IgG controls. **/## p < 0.01; ***/### p < 0.001; **/*** in relation to BSA+LPS+CF+DMSO; ##/### in relation to PA+LPS+CF+DMSO. One-way ANOVA and post hoc test (Tukey) were used for analysis.