Figure 1.

24-Well Darcy Plate (A) A 24-well plate consists of 4 injection-molded polystyrene components: a lid, media reservoir, droplet-guiding cover, and effluent collection chambers. The lid protects the media reservoirs from sources of contamination and allows the exchange of gases. The media reservoir is divided into quadrants that each can contain up to 6 mL of culture media. (B) Detailed view of an individual well where samples can be suspended in LLS. A PCTE membrane is heat-sealed against the bottom surface during the plate assembly process, securing the suspension in place. A pressure device is used to create a light-vacuum pressure within the effluent collection compartments that drives the perfusion of media through the LLS and PCTE membrane.