. 2022 Mar 8;11:e75568. doi: 10.7554/eLife.75568

Table 1. Summary of cryogenic electron microscopy (cryo-EM) data collection and image processing parameters.

Dataset	RyR1ACP/EGTA _closed	RyR1ACP/Ca2²⁺_{A inactivated}	RyR1ACP/Ca2²⁺_{A inactivated class1}	RyR1ACP/Ca2²⁺_{A inactivated class2}	RyR1ACP/Ca2²⁺_{A inactivated class3}	RyR1ACP/Ca2²⁺_{A inactivated CDTM}	RyR1ACP/Ca2²⁺_{A open}	RyR1ACP/Ca2²⁺_{A open CDTM}	RyR1ACP/Ca2²⁺_{B inactivated}	RyR1ACP/Ca2²⁺_{B open}
Data acquisition
Microscope/detector	Krios/K2	Krios/K3							Krios/K2
Voltage (kV)	300	300							300
Magnification	130,000	81,000							130,000
Defocus range (μm)	–1.2 to –2.2	–1.25 to –2.5							–1.25 to –2.5
Pixel size (Å) (calibrated)	1.06 (1.07)	1.08 (1.105)							1.06 (1.07)
Total electron dose (e/Å²)	70	70							70
Exposure time (s)	12	4.4							14
Number of frames	60	50							50
Total number of Micrographs	1959	10,002							1346
Image processing
Total number of particles selected	52,856	311,258							35,554
Final number of particles	21,551	90,530	79,277	159,444	71,499	90,530	14,012	14,012	14,669	4160
Reconstruction symmetry	C4	C4	C1	C1	C1	C4	C4	C4	C4	C4
Map resolution, FSC (0.143) (Å) (symmetry expanded map)	4.3 (3.9)	3.8 (3.5)	3.7	3.3	3.8	3.5	4.6 (4.0)	4.4	4.1 (3.8)	5.8
Map sharpening B-factor (Å²) (symmetry expanded map)	–132	–150 (–144)	–125	–102	–123	–142	–168 (–135)	–144	–111(–89)	–148
EMDB ID	22616 22597	25828	25831	25830	25832	25828^*	25829	25829^*	25833	-

Submitted as an additional map.