Table 6.
Evaluation of anti-genotoxic activity based on G and IR values for the genotoxin–2AA (5 mg/L, 1 mg/L)—and the mixture of 2AA with daidzein, equol and the combinations with minerals.
| Tested Mixture |
G
(Mean ± SD) |
IR
(Mean ± SD) |
%Anti-Genotox. |
| Negative Control | 1.00 ± 0.08 | 1.00 ± 0.10 | |
| 2AA 5 mg/L | 0.86 ± 0.09 | 2.91 ± 0.54 | |
| dai | 0.99 ± 0.13 | 2.36 ± 0.45 | 19% |
| dai + Zn | 0.89 ± 0.14 | 2.56 ± 0.78 | |
| dai + Cu | 0.94 ± 0.15 | 1.71 ± 0.28 a | 41% |
| dai + Ca | 0.98 ± 0.17 | 2.81 ± 0.66 | |
| dai + Se | 1.03 ± 0.15 | 2.49 ± 0.47 | |
| dai + Fe | 1.00 ± 0.12 | 2.03 ± 0.36 | 30% |
| eq | 1.03 ± 0.09 | 2.08 ± 0.30 | 29% |
| eq + Zn | 0.96 ± 0.06 | 2.19 ± 0.38 | 25% |
| eq + Cu | 1.01 ± 0.06 | 1.61 ± 0.32 b | 45% |
| eq + Ca | 1.04 ± 0.12 | 2.55 ± 0.50 b | |
| eq + Se | 1.08 ± 0.13 | 2.22 ± 0.40 | 24% |
| eq + Fe | 0.96 ± 0.13 | 1.80 ± 0.24 b | 38% |
| 2AA 1 mg/L | 0.92 ± 0.10 | 2.37 ± 0.48 | |
| dai | 0.92 ± 0.16 | 2.09 ± 0.45 | |
| dai + Zn | 0.88 ± 0.09 | 1.93 ± 0.25 | 19% |
| dai + Cu | 0.94 ± 0.14 | 1.54 ± 0.22 a | 35% |
| dai + Ca | 0.99 ± 0.13 | 2.15 ± 0.32 | |
| dai + Se | 1.00 ± 0.15 | 2.00 ± 0.31 | |
| dai + Fe | 0.90 ± 0.15 | 1.75 ± 0.29 a | 26% |
| eq | 0.99 ± 0.11 | 1.58 ± 0.18 | 34% |
| eq + Zn | 0.94 ± 0.10 | 1.62 ± 0.17 | 32% |
| eq + Cu | 1.03 ± 0.09 | 1.17 ± 0.14 b | 51% |
| eq + Ca | 1.09 ± 0.10 | 1.76 ± 0.23 b | 26% |
| eq + Se | 1.16 ± 0.10 | 1.80 ± 0.38 | 24% |
| eq + Fe | 1.06 ± 0.06 | 1.51 ± 0.26 | 36% |
Each value is expressed as mean ± standard deviation from at least 3 independent biological evaluations. Means that are significant different from positive control (2AA) are bolded (p > 0.05). a Significantly different from the effect of daidzein (10 μM) without minerals. b Significantly different from the effect of equol (10 μM) without minerals. %anti-genotox. of the tested samples is calculated as the inhibition of IR induced by genotoxin (2AA).