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. 2022 Feb 28;14(3):537. doi: 10.3390/pharmaceutics14030537

Figure 4.

Figure 4

Antimicrobial and cytotoxic effects of PEENPPCH containing various doses of PHMB. (A) Images of the inhibition zone of S. aureus (a–e) or S. epidermidis (f–j) generated by the PEENPPCH containing 0 (PEENPCH), 500, 1000, 2000, and 4000 ppm PHMB. Each group was photographed after treatment with the hydrogel for 24 h. Scale bar = 1.0 cm. (B) Quantitative analyses of the inhibition zones shown in (A). (C,D) Images of S. aureus (C) and S. epidermidis (D) colonies after treatment with PEENPCH or PEENPPCH containing various PHMB dosages as indicated in the figure. Images in the four rows represent the colony forming conditions of the group using bacteria with different dilutions as the seed. All images were captured 12 h after inoculation on TSB agar plates. (E) Quantitative analyses of the MPI of S. aureus or S. epidermidis shown in (C,D). Values are presented as mean ± s.d. (n = 3). * p < 0.05 compared to the S. aureus group treated with the PEENPCH. † p < 0.05 compared to the S. epidermidis group treated with the PEENPCH. (F) Toxicity of PEENPPCH containing 0 (i.e., PEENPCH), 500, 1000, 2000, or 4000 ppm PHMB to KERTr cells after treatment for 24 h. Values are presented as the mean ± s.d. (n = 3).