Table 1:
Parameter | PacBio CLR | PacBio HiFi reads | Illumina PE reads |
---|---|---|---|
Sequencer | PacBio Sequel | PacBio Sequel II | Illumina NovaSeq |
Chemistry | Sequel binding kit 3.0 | Sequel II binding kit 2.0 | TruSeq DNA Nano |
No. of SMRT cells | 6 (1M v3 cells) | 1 (8M cell) | NA |
No. of reads | 5,037,588 | 1,531,543 | 259,505,436 |
No. of bases (bp) | 90,586,242,030 | 31,312,160,541 | 77,851,630,800 |
Read length N50 (bp) | 29,274 | 20,363 | 2 × 150 |
Estimated coverage (×)a | 121 | 42 | 104 |
Accession Nos. | ERR5487554-ERR5487559 | ERR5485301 | ERR5484652 |
Based on a haploid genome size of 750 Mb. NA: not applicable.