Figure 11.

Electron microscopy and histological analysis of the changes in shrimp with infectious hypodermal and hematopoietic necrosis virus (IHHNV). (A) Electron microscopy of negatively stained IHHNV VLPs under self-assembly and disassembly conditions in Penaeus vannamei; (B) Cowdry type A eosinophilic inclusion of IHHNV in a nucleus of subcuticular epithelial cells of the pleopod of P. monodon (H & E, 1000×); (C) Histological detection of Procambarus clarkii gills negative to IHHNV detected by PCR. The gill cells were normal, no hypertrophied nucleus was observed; (D) Histological detection of P. clarkii gills positive to IHHNV detected by PCR. Several hypertrophied nuclei (arrow) were observed. ((A) Reprinted from Journal of Invertebrate Pathology, Vol. 166, Zhu, Y.P., Li, C., Wan, X.Y., Yang, Q., Xie, G.S., Huang, J., Delivery of plasmid DNA to shrimp hemocytes by infectious hypodermal and hematopoietic necrosis virus (IHHNV) nanoparticles expressed from a baculovirus insect cell system, p. 1, Copyright (2019), with permission from Elsevier; (B) Reprinted from Aquaculture, Vol. 289 (3–4), Rai, P., Pradeep, B., Karunasagar, I., Karunasagar, I., Detection of viruses in Penaeus monodon from India showing signs of slow growth syndrome, p. 5, Copyright (2009), with permission from Elsevier; (C,D) Reprinted from Aquaculture, Vol. 477, Chen, B.K., Dong, Z., Liu, D.P., Yan, Y.B., Pang, N.Y., Nian, Y.Y., Yan, D.C., Infectious hypodermal and hematopoietic necrosis virus (IHHNV) infection in freshwater crayfish Procambarus clarkii, p. 4, Copyright (2017), with permission from Elsevier).