Skip to main content
. 2022 Mar 20;14(6):1308. doi: 10.3390/nu14061308

Figure 3.

Figure 3

Effects of EF-2001 on lipid accumulation of 3T3-L1 adipocyte differentiation. (A) Effects of EF-2001 on differentiated 3T3-L1 adipocyte with ORO staining. 3T3-L1 preadipocytes were cultured with growth medium (GM) or differentiation medium (DM) in the absence or presence of EF-2001 (25–250 μg/mL) for 6 days. Lipid accumulation of 3T3-L1 adipocyte was observed by ORO staining. (B) Effects of EF-2001 on the relative lipid accumulation of 3T3-L1 adipocyte differentiation. ORO-stained 3T3-L1 cells were eluted with 100% isopropanol, and optical density (O.D.) was measured at 490 nm. Data are represented as mean ± SEM. (n = 4); ** p < 0.01 and *** p < 0.001 vs. control (without EF-2001 in differentiation medium).