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. 2022 Mar 20;14(6):1308. doi: 10.3390/nu14061308

Figure 6.

Figure 6

Effects of EF-2001 on the protein expression and nuclear translocation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes. 3T3-L1 adipocytes were cultured in the absence or presence of EF-2001 at various dose (0, 25, 50, 100, and 250 μg/mL) on MCE and insulin treated after the MCE stage for each medium condition. (A) Effect of EF-2001 on the protein level of CCAAT/enhancer binding protein (C/EBP)-α in MDI-induced 3T3-L1 adipocytes. Lysate for C/EBP-α was sampled on Day 4. The C/EBP-α immunoblot was represented by three independent replicates. (B) Effect of EF-2001 on the nuclear translocation of C/EBP-α in MDI-induced 3T3-L1 adipocytes. C/EBP-α was marked by anti-C/EBP-α primary antibody with Alexa 488 (green), and nuclei were stained with 4′6-diamidino-2-phenylindole (DAPI) (blue). The translocation of C/EBP-α was represented with confocal microscopy at 400× magnification. (C) Effect of EF-2001 on the protein level of peroxisome proliferator-activated receptor (PPAR)-γ in MDI-induced 3T3-L1 adipocytes. Lysate for PPAR-γ was sampled on Day 6. The PPAR-γ immunoblot was represented by three independent replicates. (D) Effect of EF-2001 on the nuclear translocation of PPAR-γ in MDI-induced 3T3-L1 adipocytes. PPAR-γ was marked by anti-PPAR-γ primary antibody with Alexa 488 (green), and nuclei were stained with DAPI (blue). The translocation of PPAR-γ was represented with fluorescence microscopy at 100× magnification.