Figure 5.

Effects of RAP on modulating lipogenic factors in LQG-treated 3T3-L1 cells. The cells were divided into four groups: control group, 50 μM LQG group, LQG + RAP group, and RAP-alone group. The mRNA expression levels of (A) c/ebpα, (B) pparγ, (C) srebp1c, (D) acc1, and (E) fasn were measured by RT-PCR and the protein levels of (G) PPARγ and (F) FASN were analyzed by Western blotting. Protein expression was quantified by densitometry and the relative intensities are expressed in the bar charts. Western blotting bands represent the detection of the proteins from three independent tests. Data are presented as means ± SEMs and analyzed with one-way ANOVA. * vs. control group (p ≤ 0.05); # vs. 50 μM LQG-alone treatment group (p ≤ 0.05).