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. 2022 Mar 3;11(3):313. doi: 10.3390/pathogens11030313

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Schematic representation of the procedure to establish the InfectionCMA. The infection assay can have variable timelines depending on the experimental assay. The manual establishment and immune processing of the InfectionCMA take around 5–7 days. A 1–2 cm pellet of infected cells guarantees 30 slides from each InfectionCMA. This biological resource can be used for the expression evaluation of several markers of interest, through diverse techniques such as immunocytochemistry (ICC) and in situ hybridization (ISH).