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. 2022 Mar 11;25(4):104059. doi: 10.1016/j.isci.2022.104059

Figure 5.

Figure 5

AMSC potency in response to preconditioning in a 0.2% collagen gel and 2D monolayer at hypoxic oxygen tension

(A) Chondrogenic micromasses were stained with Alcian Blue to detect sulfated GAGs (Top, scale bar = 5 mm) after 7 days and SOX9 expression analyzed by qPCR (Bottom).

(B) An osteogenic differentiation assay was conducted for 21 days, illustrating calcium phosphate staining using Alizarin Red (Top) and gene expression of osteogenic associated transcription factor RUNX2 analyzed by qPCR (Bottom).

(C) An adipogenic differentiation assay was cultured over 21 days with the presence of fat droplets analyzed using Oil Red O stain (Top, scale bar = 10 μm) and FABP4 gene expression analyzed (Bottom). (Data are presented as the mean ±S.E.M, statistical analysis performed using Mann-Whitney test; ∗∗p < 0.01; ∗p < 0.05; n = 3).