Myopathy with oval inclusions in a domestic shorthair cat

Abstract

Case summary

An 18-month-old castrated male domestic shorthair cat was presented with a 2-month history of collapse and severe weakness, particularly affecting the pelvic limbs. A biceps femoris muscle biopsy revealed excessive variability in myofibre size, mild necrosis, minimal centronucleation and scattered 10 μm intracytoplasmic oval inclusions. The inclusions appeared amphophilic with haematoxylin and eosin, blue with Gomori trichrome and unstained with nicotinamide adenine dinucleotide dehydrogenase tetrazolium reductase staining. ATPase staining revealed a normal mosaic pattern and atrophy of both type 1 and 2 myofibres. The pathological diagnosis was a myopathy with inclusions. In contrast to previous feline myofibre inclusions previously reported in the literature, inclusions were not identified after immunohistochemistry using anti-desmin, tubulin, spectrin, laminin, LAMP and LC3 antibodies. After supportive care and corticosteroid treatment, clinical improvement was noted and the cat was discharged 10 days after initial presentation. Clinical and neurological re-examinations were performed at 1, 3, 6 and 9 months after discharge. Owner contact at both 10 and 30 months post-discharge confirmed that persistent muscular weakness was present.

Relevance and novel information

This case report describes a novel and slowly progressive feline myopathy associated with oval amphophilic inclusions unreactive to immunostaining, which have not been previously reported in feline myopathies.

Introduction

Feline muscular weakness is reported in multiple acquired conditions such as myasthenia gravis, myositis and polyneuropathies.^1–3 In other cases, feline muscular weakness can be associated with hereditary musculoskeletal disorders. In humans, biomolecular and genetic mutation tests have enabled precise classification of hereditary muscular disorders. In the cat, a diagnosis is often based on histological changes observed on a muscle biopsy and associated supportive signs.^4–13

This case report describes the clinical and histopathological findings of a cat that was initially presented with muscular weakness. A muscle biopsy revealed sarcoplasmic oval inclusions associated with fibre atrophy, centronucleation and mild inflammation. Immunostaining did not show any similarities with the other feline congenital muscular disorders previously described in the literature. Symptomatic treatment led to clinical improvement, but mild clinical signs persisted after discharge. Even if the definitive biomolecular abnormality ultimately remained unknown, this case report describes atypical inclusions in myofibres that have not previously been described in the cat.

Case description

An 18-month-old male castrated and vaccinated domestic shorthair cat was presented with a 2-month history of prolonged periods of recumbency. Litter mates were reported to be healthy at the time of diagnosis. Muscle weakness, particularly affecting the pelvic limbs, an abnormal gait with episodes of collapse, difficulties in swallowing and a progressive onset were reported. Two weeks prior to referral the cat deteriorated acutely and aphonia was reported. The cat was stabilised with fluid therapy and analgesics prior to referral.

On physical examination, severe paresis was present; the cat was unable to stand even with assistance and presented in lateral recumbency. Generalised pain, hypothermia (36.8 ºC) and bradycardia (80 beats per min) were observed. Mild pain was identified on palpation of all muscles. Normal proprioception and hypotonia were observed on the four limbs, pelvic limb reflexes were diminished and cranial nerve examination revealed a bilaterally decreased palpebral reflex. Differential diagnoses included muscular disorders (including myopathies and myositis), neuromuscular junction disorders (myasthenia gravis, botulism, polyradiculoneuritis) and polyneuropathies (congenital, infectious, ischaemic neuromyopathy or paraneoplastic disorders). Polyarthritis and meningitis, although considered less likely were included in the differential list.

Haematology and electrolytes were within the reference intervals (RIs). Most biochemical parameters were within the RIs except for serum creatine kinase and lactate dehydrogenase, which were both slightly increased (605 UI/l [RI 0–340] and 2136 UI/l [RI 0–180], respectively). Arthrocentesis of both carpi and tarsi revealed grossly normal synovial fluid (volume, colour and viscosity) and, as such, cytological evaluation was not performed. A lumbar cerebrospinal tap was performed and cerebrospinal fluid proteins (0.1 g/l) and cell count (0 cell/µl) were within the RIs. A neostigmine test (neostigmine 0.04 mg/kg IM [Prostigmine; Mylan]) did not result in any clinical improvement in the 6 h following the injection. The neostigmine test is not 100% sensitive for myasthenia gravis, so an acetylcholine receptor antibody test was recommended but refused by the owners on financial grounds. Serological tests for feline leukaemia antigen, feline immunodeficiency virus antibody and toxoplasma antibodies were negative. No abnormalities were detected on thoracic and spinal radiographs.

Fluid therapy (lactated Ringer’s 3 ml/kg/h IV) and analgesia (buprenorphine 20 µg/kg SC q8h [Vetergesic NDV, Ceva]) were administered. Prednisolone (0.5 mg/kg PO q12h [Prednicortone; Dechra]) therapy was initiated as an inflammatory myopathy or myositis were considered possible. After 10 days of treatment, the cat was pain-free and ambulatory. Limb proprioception and palpebral reflexes were normal. Muscular weakness was still present with frequent episodes of recumbency after any exercise.

An underlying myopathy was suspected. Once the cat was considered stable for anaesthesia, a surgical muscle biopsy was obtained. Under anaesthesia (diazepam 0.25 mg/kg IV [Diazepam; TVM]; alfaxalone 4 mg/kg IV [Alfaxan]; morphine 0.1 mg/kg IV [Cooper]; isoflurane [Isovet]), a biopsy from the left biceps femoris muscle was obtained and sent immediately for histological evaluation at 4 ºC. On arrival at the laboratory, the muscle biopsy was snap frozen in isopentane cooled with liquid nitrogen, prior to transverse serial sectioning (8 µm thick). Routine staining techniques were utilised: haematoxylin, eosin and saffron (HES), Gomori trichrome, periodic acid–Schiff and histoenzymological procedures (nicotinamide adenine dinucleotide dehydrogenase tetrazolium reductase [NADH-TR]) and ATPase following preincubation at pH 4.2, 4.63 and 10.4 for fibre typing. ¹⁴ Histopathological analysis was performed by a board-certified veterinary pathologist.

Histopathological analysis revealed generalised changes in the muscle, characterised by excessive variability in myofibre size with occasional hypertrophic fibres (up to 80 µm) and numerous atrophic fibres (<20 μm), some of which had fragmented or clear sarcoplasm associated with ongoing necrosis (Figure 1). Ten percent of fibres had central myonuclei, consistent with mild regenerative response. Centronucleation was not observed in any of the C-type fibres. Inflammation was mild and mainly comprised mononuclear cells surrounding necrotic fibres. Fibrosis was absent. Approximatively 1–2% of fibres showed a single 10 μm intrasarcoplasmic, centrally located oval-shaped inclusion. Fibres containing more than one inclusion were rarely observed. All inclusions were similar in appearance, amphophilic with HES staining, staining navy blue with Gomori trichrome and unstained with both NADH-TR reagent and periodic acid–Schiff (Figures 1 and 2). ATPase typing did not show any alteration in either normal or atrophic fibre type distribution. Inclusions were present both in type 1 and 2 fibres. Fibre atrophy, centronucleation and mild necrosis without any fibre-type grouping was consistent with a primary muscle pathology. Histopathological diagnosis was consistent with a myopathy with intrasarcoplasmic inclusions.

Figure 1.

Muscular histopathological changes. Transverse sections of a fresh-frozen muscle biopsy from the left biceps muscle stained with (a–c) haematoxylin, eosin and saffron (HES) and (d–e) Gomori trichrome navy blue staining (GT). (a,d) × 100; (b,e) × 200; (c,f) × 400. Atrophic fibres were observed (yellow and white arrows) and most contained inclusions, which appeared amphophilic on HES (white arrowheads) and blue on GT (yellow arrowheads)

Figure 2.

Morphology of the inclusions. Transverse section of a fresh-frozen muscle biopsy from the left biceps muscle (× 400). (a) Haematoxylin, eosin and saffron staining: a 10 µm intrasarcoplasmic oval shape amphophilic inclusion is located at the centre of a myofibre (arrowhead). (b) Nicotinamide adenine dinucleotide dehydrogenase tetrazolium reductase reaction staining: two atrophic myofibres exhibit similar oval-shaped inclusions in the form of unstained halos (arrowheads)

Additional muscle sample sections were used for immunohistochemistry. Antibody markers used in the study are summarised in Table 1. Negative and positive controls were obtained, with an additional muscle section, with omission of the primary antibody, and with a section from a healthy feline biceps femoris muscle. Tissue distribution and cellular localisation of these proteins were unchanged. The inclusions were not stained by any of the immunohistochemical markers. Rare intrasarcoplasmic figures of macrophages identified by a LC3-positive signal was consistent with phagocytosis induced by necrosis (Figure 3).

Table 1.

Proteins and corresponding antibodies used for immunostaining

Protein	Antibody reference	Dilution	Laboratory
Laminin	Rabbit polyclonal antibody, L9393	1:100	Sigma-Aldrich
	Mouse monoclonal antibody, D5	1:50	Developmental Studies Hybridoma Bank
Alpha tubulin	Mouse monoclonal antibody GTX628802, clone GT114	1:200	Gentex Corporation
Beta tubulin	Mouse monoclonal antibody MMS-435P, clone TU51	1:500	BioLegend
Desmin	Mouse monoclonal antibody M0760, clone D33	1:200	Dako
Spectrin	Mouse monoclonal antibody NCL-SPEC1	1:100	Novocastra Laboratories
LAMP	Mouse monoclonal antibody ab25082	1:400	Abcam
LC3	Rabbit polyclonal antibody L7543	1:100	Sigma-Aldrich

Figure 3.

LC3 immunostainings of a transverse section of a fresh-frozen muscle biopsy from the left biceps muscle (× 400). Inclusions (arrowheads) are distinguishable but are not marked by LC3 antibodies. A macrophage located inside the fibre (arrow) is marked by LC3, suggesting macrophage phagocytosis secondary to fibre necrosis. Nuclei counterstained in blue and phase contrast to depict fibre limits

Two days postoperatively, the cat was discharged on prednisolone (0.5 mg/kg PO q12h for 21 days and 50% tapering doses every 7 days). Corticosteroid therapy was continued owing to a perceived improvement in clinical signs associated with treatment. Re-examinations were performed at 1, 3, 6, 9 and 30 months postoperatively. The owners reported some improvement in clinical signs over this time period; however, intermittent periods of collapse, exercise intolerance, polypnoea and prolonged recovery from exercise were still present. During all re-examinations between 1 and 30 months, the cat was exercise intolerant but was no longer recumbent. The creatine kinase blood level was 113 U/l and 688 U/l at 3 and 6 months, respectively. At the time of writing (>2 years after initial diagnosis), the cat had stable disease without any ongoing treatment.

Discussion

In this case, muscular weakness prompted a muscle biopsy, which subsequently revealed a myopathy with inclusions. Muscular weakness remained after discharge, but the cat had stable disease for at least 4 years post-diagnosis.

Slowly progressive to static evolution is commonly reported in human congenital myopathies, ¹¹ and has been described in feline nemaline rod congenital myopathy. ⁶ Comparatively, the life expectancy of cats with feline dystrophies is generally less than 3 years.^4,12,13 Clinical signs were widely dominated by muscular weakness. Muscular weakness is the most commonly observed clinical sign in both human and feline muscular disorders.^{3,5,7,9,11–13,15,16} In this study, we also observed abnormal gait, episodes of collapse, dysphonia/aphonia and difficulty in swallowing, which have been reported in other feline myopathies.^6,7,9,15 However, we did not observe stridor, tremor or cervical ventroflexion, which have also been reported.^6,9,15 Some signs more suggestive of congenital human myopathies were also observed in our study, such as hyporeflexia, hypotonia and absence of mental alteration. ¹¹ Creatine kinase was only mildly increased in our study. This parameter is generally increased in other feline muscular disorders, such as muscular dystrophy, ⁴ toxoplasmosis, ¹ necrotising myopathy ⁵ or nemaline rods myopathy. ⁶ Clinical evaluation is currently the best way to assess the severity of the disease as there are no blood parameters that accurately reflect the severity of the muscular lesions.^{5,13,15,17,18} Except for hypokalaemic myopathy, ¹⁹ no specific treatment is available for myopathic cats, and only supportive care can be given. Corticosteroids can improve some inflammatory or necrotising myopathies in both cats and in humans.^5,20

A fresh biceps femoris biopsy was obtained, which is a good option for feline muscle examination. ²¹ Histopathological changes were dominated by atrophy of both type 1 and 2 fibres and mild necrosis. These findings excluded feline dystrophic disorders, which are dominated by replacement of muscle fibres by adipose and connective tissue.^8,10,12,13 Significant centronucleation (>3%) was observed in our study, but it was less severe than would usually be identified in human or canine centronuclear myopathies;^11,22,23 these changes suggested regeneration secondary to mild necrosis. The most specific morphological alterations observed were 10 μm intra-sarcoplasmic oval-shaped inclusions, which were amphophilic in HES and navy blue with Gomori trichrome stain. The other muscular disorders that can show fibre inclusion in humans were not consistent with the epidemioclinical findings of our study.^24–26 Infectious causes and neoplastic changes were not identified. Inclusions had a different morphology than feline nemaline rods^6,9 or alpha glucosidase deficiency, ⁷ and affected both fibre types, contrary to that seen in tubulin-inclusion myopathy, ¹⁵ which has not previously been reported in either humans or cats (Table 2).

Table 2.

Comparative pathology of different feline myopathies reporting sarcoplasmic inclusions

Myopathy	Nemaline rods myopathy	Glycogen storage IV	Tubulin reactive inclusions	Oval amphophilic inclusion bodies
Authors’ description	Cooper et al, 6 Kube et al 9	Fyfe 7	Shelton et al 15	The present study
Underlying effects	Possible surplus of Z-band material	Glycogen branching enzyme deficiency	Unknown	Unknown
Inheritance	Possibly autosomal recessive	Autosomal recessive	Unknown	Unknown
Epidemiology	DSH (USA, Belgium)	Norwegian Forest Cat (USA, Europe)	Devon Rex, DSH (USA)	DSH (France)
Onset	6–18 months	<5 months	<8 months	16 months
Age of death	<2 years	9–13.5 months	>4 years	>20 months
Clinical signs
Abnormal gait	Wobbly hypermetric	Bunny hopping	Loss of balance and collapse	Loss of balance and collapse
Muscular weakness	Exercise intolerance Recumbency after walking (Cooper et al6)	Prolonged decubitus	Mild cervical ventroflexion Prolonged decubitus	Muscular weakness Prolonged decubitus
Muscle atrophy	Progressive	Progressive	Mild to moderate	–
Difficulties in feeding	+ (Kube et al9)	+	+	+
Tremor	+	+	–	–
Myotatic reflexes	Decreased	Decreased	Normal	Decreased to normal
Comorbidities	Weak voice (Cooper et al6)	Stillbirth Cardiomyopathy Persistent hyperthermia	Upper respiratory infection	Intermittent aphonia
Histological lesions
Atrophy	+	+	–	+
Necrosis	+ (Cooper et al6)	+	–	+
Fibrosis	+ (Cooper et al6)	+ (myocardium)	–	–
Elevated centronucleation	+ (Cooper et al6)	+	–	+
Fibre type mostly affected	2A	Unspecified	2B	Both equal
Inclusion morphology	Rod-like: very few to filling the whole fibre H&E: not obvious Gomori trichrome: red (Cooper et al6) to blue (Kube et al9)	Numerous clusters of round shapes H&E: pale blue PS: red purple	Oval: mostly 1 to a few in a fibre H&E: eosinophilic Gomori trichrome: bright red PAS: positive around the inclusions NADH/SDH: dark blue rim around the inclusions	Oval: 1 to a few in a fibre H&E: amphophilic Gomori trichrome: purple NADH: unstained halo around inclusions
Immunofluorescences abnormalities	Sarcoplasmic accumulation of dystrophin, desmin and spectrin Diffuse alpha-actinin distribution	Not realised	Tubulin reactive inclusion Normal distribution of desmin, amyloid, ubiquitin and MLF1	Normal distribution of desmin, tubulin, spectrin, laminin, LAMP and LC3 Positive LC3 activated macrophages

DSH = domestic shorthair; H&E = haematoxylin and eosin; PAS = periodic acid–Schiff; NADH = nicotinamide adenine dinucleotide dehydrogenase; SDH = succinic deshydrogenase; MLF1 = succinic deshydrogenase; + = present; – = absent

In humans, biomolecular and genetic testing provides a more precise classification of hereditary muscular disorders, such as dystrophies, congenital myopathies, myotonic dystrophies, myasthenic syndrome and metabolic myopathies. ¹¹

In humans, sarcoplasmic inclusions associated with congenital myopathies (such as rods or cores) are differentiated from other inclusions using immunostaining. ¹¹ Several antibodies used in the diagnosis of human myopathies are available and have been found to cross-react with feline muscle proteins. ²⁷ In this study, antibodies previously tested in feline dystrophies or myopathies, such as desmin, tubulin, spectrin and laminin were used.^9,12,13,15 Sarcoplasmic desmin accumulation and diffuse alpha-actinin distribution were not observed in our case, whereas they are associated with feline and human nemaline rod myopathy.^9,11 In feline tubulin and human core myopathies, inclusions are highlighted by tubulin and desmin, respectively.^11,15 In our study, distribution of these proteins was similar to the control. LAMP and LC3, two autophagy-related proteins that have been highlighted in some human myopathies, were also used.^28,29 In our study, macrophages were positive for LC3, but no inclusions were highlighted by this antibody.

Genetic diagnosis is available for three feline muscular hereditary disorders: COLQ for Devon Rex and Sphynx congenital myasthenic syndrome; CLCN1 for myotonia; and WNK4 for periodic hypokalaemic polymyopathy.^19,30,31 Feline myopathy classification is currently lacking, owing to a lack of tests to identify mutations.

Limitations of this case report include the absence of identification of the biochemical nature of the inclusion. Electron microscopy has been previously used to characterise nemaline rods and glycogen storage myopathies,^6,7,9 and would have been useful here to determine the nature of the inclusions. The current lack of specific immunostaining described in the literature to characterise feline muscular disorders has also limited the exploration of this myopathy.^9,12,13,15 An additional limitation was the absence of electromyography, nerve biopsy or MRI, which may have allowed us to exclude a neuropathic disorder with more certainty.

Conclusions

This study describes a slowly evolving muscular disorder in the cat, characterised histopathologically by atrophy and sarcoplasmic inclusions. Given the ‘one medicine’ approach, better characterisation of feline muscular disorders using molecular biology and mutation identification must be encouraged in the future.