Figure 8.

By flow cytometry, in the bone marrow of wild-type mice, GFP is expressed only by cells in the LSK gate, while in that from Gata1^low mice, GFP is expressed both by non-LSK and LSC cells. Scatter plots of bone marrow cells from representative huCD34-GFPH2B and huCD34-GFPH2B/Gata1^low littermates showing the gates used to identify cKIT⁻ cells (orange events), LSK (purple events), and SLAM (brown events) cells, as indicated. The intensity of the GFP expressed in the different populations is shown as color-coded histograms below each panel. The dotted histogram included in each panel indicates the signal in the GFP channels from the corresponding cells from the bone marrow of hCD34 and hCD34/Gata1^low mice, used as negative controls. Abbreviations: CD117: cluster of differentiation 117 which recognize cKIT, the receptor for stem cell factor; LSK: cells that do not express lineage markers but express cKIT and Sca1; SLAM: LSK which also express markers of the signaling lymphocyte activation molecules (SLAM) family.