Skip to main content
. 2022 Feb 11;11:e72872. doi: 10.7554/eLife.72872

Video 12. Monopolar spindles break down and individual chromosomes segregate following dynein depletion.

Download video file (481.1KB, mp4)

Shown is an oocyte from the Dynein AID strain expressing GFP::tubulin and GFP::histone following klp-18(RNAi) to generate monopolar spindles, dissected into Meiosis Medium containing 100 μM auxin to deplete dynein. Upon dissection into auxin solution, the monopole quickly dissolves, leading to ejection of individual chromosomes (with laterally associated microtubule bundles) into the cytoplasm, followed by a synchronous anaphase-like segregation event. Corresponds to Figure 4A. Phenotypes are consistent across all oocytes filmed (n = 5). Time elapsed shown in min:s. Scale bar = 5 μm.