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. 2022 Jan 18;13(2):2878–2888. doi: 10.1080/21655979.2022.2025695

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — ARHGAP9 overexpression inhibited the proliferation of lung adenocarcinoma cells. (a) ARHGAP9 had a low expression in non-small cell lung cancer cell lines analyzed by CCLE database. The protein (b) and mRNA (c) expressions of ARHGAP9 were measured with Western blot and RT-qPCR. *P < 0.05 and ***p < 0.001 vs 16HBE. The protein (d) and mRNA (e) expressions of ARHGAP9 after transfection were measured with Western blot and RT-qPCR. (f) The A549 cell viability was detected by using CCK-8. (g) The proliferation and tumorigenesis ability of A549 cells were detected using colony formation assay (magnification, x100). (h) The proliferation of A549 cells was detected by using EDU staining (magnification, x200). ***p < 0.001 vs Ov-NC.