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. 2022 Jan 18;13(2):2840–2850. doi: 10.1080/21655979.2021.2024687

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — EX 527 reverses the effects of liquiritin on cell proliferation in ox-LDL-induced hVSMCs. hVSMCs were treated with 80 μM liquiritin or/and 10 μM EX 527 in the presence of 100 μg/mL ox-LDL for 24 h. An MTT assay was performed to measure the proliferation of ox-LDL-induced hVSMCs. (b) Caspase-3 activity of ox-LDL-induced hVSMCs was determined using a colorimetric assay kit. (c, d) The cell apoptosis rates of ox-LDL-induced hVSMCs were measured by FCM analysis. (e, f) The protein expression levels of Bcl-2 and Bax in ox-LDL-induced hVSMCs were detected by Western blot analysis and quantified using ImageJ software. **P < 0.01 vs. ox-LDL; ^## P < 0.05, 0.01 vs. ox-LDL+liquiritin group.