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. 2022 Apr 1;11:e67598. doi: 10.7554/eLife.67598

Figure 1. Design and action of auxin-inducible gene expression system (AGES).

A tubulin promoter ubiquitously expresses the bicistronic TIR1 and AGES cassette. Auxin-inducible degron (AID)-tagged GAL80 is cleaved from the TIR1 due to the presence of the T2A sequence. In the absence of auxin, GAL80 can inhibit GAL4 activity, whilst the presence of auxin induces degradation of GAL80, allowing GAL4 to drive the expression of UAS-transgene(s).

Figure 1.

Figure 1—figure supplement 1. Plasmid map of the auxin-inducible gene expression system (AGES) plasmid.

Figure 1—figure supplement 1.

Map of the pattB-tubP-AtTIR1-P2A-miniAID-Gal80-miniAID-SV40 plasmid (generated using Snapgene). The full sequence is in Figure 1—figure supplement 1—source data 1.
Figure 1—figure supplement 1—source data 1. Genbank (.gb) sequence file for pattB-tubP-AtTIR1-P2A-miniAID-Gal80-miniAID-SV40.