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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Biomaterials. 2022 Feb 28;283:121450. doi: 10.1016/j.biomaterials.2022.121450

Figure 7.

Figure 7.

Cells transfected with the MT1-MMP FRET biosensor showed the MMP activity of cells infiltrating a Matrigel ECM at low (<10°) and high (>20°) angles of indentation (Φ) when cells invade and deform a 3D ECM; MMP activity was higher for the higher angle of indentation as seen by the heat map in (A) and mean FRET ratios presented in (B), The degradation of the Matrigel substrate correlated with the activity of MMP enzymes by analyzing the growth media via zymography (fluorogenic peptide assay) showed activity of MMPs for negative controls (NC), MDA-MB-231 cells (231), and positive controls (PC) (C). A: Reproduced with permission from reference [60], Copyright (2014) Elsevier.