Fig. 5.

Ly6G⁺ myeloid cells depletion enhanced sorafenib efficacy and reduced fibrosis. (A) Tumor volume was measured using a Vernier caliper, V = a × b²/2, where a is the long diameter and b is the short diameter( n = 5, two – way ANOVA). (B) Number of MDSC were measured using flow cytometry(n = 3). (C) IL-6 immunostaining images of tumor tissues (Scale bar = 100 μm.). Positive area was analyzed by ImageJ(n = 3). (D) Masson staining of tumor tissue(Scale bar = 200 μm).Collagen volume fraction was analyzed by ImageJ(n = 3). Specific n values of biological independent animals. ANOVA for four groups comparisons and Tukey test for multiple comparisons. Asterisks indicate comparison with the control group. *p < 0.05, **p < 0.01, ***p < 0.001.