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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Hepatology. 2021 Dec 13;76(1):78–93. doi: 10.1002/hep.32196

Figure 4: Effects of Rev-erbα, ApoAIV, and Crebh deficiencies on plasma lipoproteins and apolipoproteins.

Figure 4:

(A-B) Male 12-week-old Nr1d1−/− and Nr1d1+/+ mice were fed a chow diet. (A, top) Plasma triglyceride levels were quantified at 24:00 and 12:00. (A, bottom) Apolipoprotein levels in plasma collected at 12:00 were measured via Western blotting. (B) Isolated lipoproteins were analyzed by electron microscopy. Scale bar = 50 nm.

(C-D) Male 16-week-old Apoa4−/− and Apoa4+/+ mice were fed a chow diet. (C) Triglyceride levels were measured at 24:00 and 12:00 in total plasma (top). Plasma obtained at 12:00 was used to detect apoB and apoA1 levels (bottom), and (D) were used to isolate different lipoproteins for negative staining. Scale bar = 50 nm.

(E-F) Male 16-week-old Creb3l3−/− and Creb3l3+/+ mice on a chow diet were sacrificed at different times. Plasma was used (top) to measure total triglyceride and cholesterol, Values are mean ± SD. *** p<0.001, t-test, and (bottom) to detect apolipoproteins by western blotting. (F) Lipoproteins were isolated at 12:00 and for negative staining. Scale bar = 50 nm.