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[Preprint]. 2022 Apr 6:2022.04.06.487325. [Version 1] doi: 10.1101/2022.04.06.487325

Delta-Omicron recombinant SARS-CoV-2 in a transplant patient treated with Sotrovimab

Ralf Duerr, Dacia Dimartino, Christian Marier, Paul Zappile, Guiqing Wang, Jonathan Plitnick, Sara B Griesemer, Erica Lasek-Nesselquist, Meike Dittmann, Mila B Ortigoza, Prithiv J Prasad, Kirsten St George, Adriana Heguy
PMCID: PMC8996620  PMID: 35411351

Summary

We identified a Delta-Omicron SARS-CoV-2 recombinant in an unvaccinated, immunosuppressed kidney transplant recipient who had positive COVID-19 tests in December 2021 and February 2022 and was initially treated with Sotrovimab. Viral sequencing in February 2022 revealed a 5’ Delta AY.45 portion and a 3’ Omicron BA.1 portion with a recombination breakpoint in the spike N-terminal domain, adjacent to the Sotrovimab quaternary binding site. The recombinant virus induced cytopathic effects with characteristics of both Delta (large cells) and Omicron (cell rounding/detachment). Monitoring of immunosuppressed COVID-19 patients treated with antiviral monoclonal antibodies is crucial to detect potential selection of recombinant variants.

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