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. 2022 Apr 12;11:e67621. doi: 10.7554/eLife.67621

Figure 2. Three months of JUUL aerosol inhalation leads to an increase of inflammatory mediators HMGB1 and RAGE.

Brains were harvested at the end point and the regions for NAc-core, NAc-shell and Hippocampus were sectioned. Later, protein was extracted and Western Blot was performed to quantify the expression of HMGB1-1 and RAGE. HMGB1-1 relative protein level are shown from NAc-core at (A) 1 month and (B) 3 months, from NAc-shell at (C) 1 month and (D) 3 months, and from Hippocampus at (E) 1 month and (F) 3 months. RAGE protein levels are shown from NAc-core at (G) 1 month and (H) 3 months, from NAc-shell at (I) 1 month and (J) 3 months, and from Hippocampus at (K) 1 month and (L) 3 months. Changes in proteins levels are relative to Air controls. Data are presented as individual data points ± SEM with n = 5–6 mice per group. *p < 0.05, **p < 0.01 and *** p < 0.001.

Figure 2.

Figure 2—figure supplement 1. Inflammatory protein level changes in the central nervous system in mice exposed to JUUL Mango and JUUL Mint.

Figure 2—figure supplement 1.

These data are graphed with each exposure type (air – blue, mango – orange, and mint – green) grouped for ease of comparison of protein levels at 1 month (white columns) and 3 months (solid columns) of exposure. Data were analyzed with one-way ANOVA with Holm-Sidak multiple comparisons for each brain region and protein of interest. Data are presented as individual data points ± SD with n = 5–6 mice per group. *p < 0.05, **p < 0.01, and *** p < 0.001.