Figure 6. Haploinsufficiency of Ezh2 rescues cleft palate in Wnt1^Cre;Kdm6b^fl/fl;Ezh2^fl/+ mice.

(A–C) CT images at PN0.5. White arrowheads in (A, C) indicate palatine process of maxilla (PPM) observed in control and Wnt1^Cre;Kdm6b^fl/fl;Ezh2^fl/+ rescue model. Red arrows in (A, C) indicate palatine bone observed in control and Wnt1^Cre;Kdm6b^fl/fl;Ezh2^fl/+ rescue model. White asterisk in (B) indicates missing palate PPM in Wnt1^Cre;Kdm6b^fl/fl mice, and red asterisk indicates missing palatine bone in Kdm6b mutant mice. Scale bar: 0.4 mm. (D–F) Coronal views of CT images at PN0.5. Asterisk in (E) indicates cleft palate observed in Wnt1^Cre;Kdm6b^fl/fl mice. Scale bar: 0.3 mm. (G–I) Whole-mount oral view at PN0.5. Arrowhead in (H) shows complete cleft palate observed in Wnt1^Cre;Kdm6b^fl/fl mice. Dashed lines in (G–I) indicate location of sections in (J–O). Scale bar: 2 mm. (J–O) Histological analysis of samples at PN0.5. Asterisk in (K, N) indicates cleft palate in Wnt1^Cre;Kdm6b^fl/fl mice. (M–O) are magnified images of boxes in (J–L), respectively. Dotted lines in (M–O) outline the bone structure. Scale bar: 200 µm. Mes: mesenchyme.