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. 2022 Mar 7;41(16):2303–2314. doi: 10.1038/s41388-022-02233-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Schematic overview of the development of the AML-MSCV and AML-IRF7 models. B, C Equal numbers of AML-MSCV and AML-IRF7 cells were transplanted into recipient mice. The percentage of peripheral blood GFP⁺BFP⁺ cells was monitored every 5 days (n = 4) (B), and the survival of mice is shown in Kaplan–Meier curves (n = 10) (C). D–F The mice were intravenously injected with AML-MSCV or AML-IRF7 cells and sacrificed at the middle stage of AML. D BrdU and 7-AAD staining was performed. Representative results are shown (upper), and the percentages of G0/G1, S, and G2/M phase AML cells are plotted (lower). E The expression of c-kit in AML cells was analyzed. Representative results are shown (upper), and the percentage of c-kit⁺ AML cells is plotted (lower). F Secondary colony formation experiments were performed by seeding 500 sorted AML cells per well into 24-well plates. Representative results are shown (upper), and the colony numbers are plotted (lower). Data are presented as mean ± S.E.M. Unpaired Student’s t test, one-way ANOVA tests and Kaplan–Meier estimates were used. **p < 0.01; ***p < 0.001.