Figure 4.
Ad26.COV2.S vaccine protects Syrian hamsters against challenge with WA1/2020 and B.1.621
(A) Experimental setup.
(B) Serum anti-S protein antibody response (EC50) in control hamsters (black symbols) and hamsters immunized with 108 (red symbols) or 1010 (blue symbols) of Ad26.COV2.S (∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ns = not significant by non-parametric one-way ANOVA with a Dunn’s post-test).
(C) Serum neutralizing titer (IC50) against WA1/2020 (circles) or B.1.621 (triangles) from hamsters immunized once with 108 (red symbols) or 1010 (blue symbols) of Ad26.COV2.S (∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ns = not significant by non-parametric one-way ANOVA with a Dunn’s post-test).
(D–G) Syrian hamsters were challenged with 103 PFU of the WA1/2020 (circles) or B.1.621 (triangles), and nasal washes (D and E) and lungs (F and G) were evaluated for viral RNA levels by qRT-PCR (D and F) and infectious virus by plaque assay (E and G) (∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, ns = not significant by one-way ANOVA with a Dunnett’s [D and F] or Dunn’s [E and G] post-test).
(B–G) Bars indicate the geometric mean values, and dotted lines are the LODs of the assays. Animals at the limit of detection are arbitrarily assigned this value. These values are combined with those having values above the limit to determine the GMT. The results are from one experiment, and each symbol represents an individual animal.
See also Figures S1 and S4.