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. 2022 May;28(5):756–765. doi: 10.1261/rna.079050.121

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© 2022 Shin et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — TAIL-seq analysis of poly(A) tail length in cultured neurons depleted of CPEB1. (A) Distribution of poly(A) tail lengths of RNAs versus fraction of genes in CPEB1-deficient rat hippocampal neurons as determined by TAIL-seq. (B) Distribution of poly(A) tail length versus fraction of sequence tags in CPEB1-deficient hippocampal neurons. (C) Box plots of median poly(A) tail distribution in biologic replicates of CPEB1-deficient neurons (CPEB1) compared to a nonspecific siRNA control (NS1 NS2) (Wilcoxon rank sum test after Bonferroni correction method). (D) Scatter plot of poly(A) tail lengths in CPEB1-depleted neurons. (E) Motif analysis of RNAs whose poly(A) tails were shortened following CPEB1 depletion. The boxed motif contains the putative binding site for CPEB1. (F) Distribution of poly(A) of SPARC RNA in CPEB1-deficient neurons. (G) PAT assay of SPARC RNA performed in biologic triplicate from CPEB1-deficient neurons.